Zobrazeno 1 - 6
of 6
pro vyhledávání: '"Ane Beth Sloth"'
Autor:
Danna Kamstrup Sell, Babak Bakhshinejad, Anders Wilgaard Sinkjaer, Ida Melissa Dawoodi, Mette Neiegaard Wiinholt, Ane Beth Sloth, Camilla Stavnsbjerg, Andreas Kjaer
Publikováno v:
Current Issues in Molecular Biology, Vol 46, Iss 9, Pp 10590-10605 (2024)
Phage display has been widely used to identify peptides binding to a variety of biological targets. In the current work, we planned to select novel peptides targeting CD4 through screening of a commercial phage display library (New England Biolabs Ph
Externí odkaz:
https://doaj.org/article/b523ef529ddc46bbafa08b887229244f
Autor:
Ane Beth Sloth, Babak Bakhshinejad, Malte Jensen, Camilla Stavnsbjerg, Mikkel Baldtzer Liisberg, Maria Rossing, Andreas Kjaer
Publikováno v:
Viruses, Vol 14, Iss 11, p 2402 (2022)
The principal presumption of phage display biopanning is that the naïve library contains an unbiased repertoire of peptides, and thus, the enriched variants derive from the affinity selection of an entirely random peptide pool. In the current study,
Externí odkaz:
https://doaj.org/article/fafcebfb51ba415e8d9afd18b55b987e
Publikováno v:
Sloth, A B, Bakhshinejad, B, Stavnsbjerg, C, Rossing, M & Kjaer, A 2023, ' Depth of Sequencing Plays a Determining Role in the Characterization of Phage Display Peptide Libraries by NGS ', International Journal of Molecular Sciences, vol. 24, no. 6, 5396 . https://doi.org/10.3390/ijms24065396
International Journal of Molecular Sciences; Volume 24; Issue 6; Pages: 5396
International Journal of Molecular Sciences; Volume 24; Issue 6; Pages: 5396
Next-generation sequencing (NGS) has raised a growing interest in phage display research. Sequencing depth is a pivotal parameter for using NGS. In the current study, we made a side-by-side comparison of two NGS platforms with different sequencing de
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::da7dafd7aa1f0407c9d927582f68ecfc
https://doi.org/10.3390/ijms24065396
https://doi.org/10.3390/ijms24065396
Publikováno v:
He, Y, Døssing, K B V, Sloth, A B, He, X, Rossing, M & Kjaer, A 2023, ' Quantitative Evaluation of Stem-like Markers of Human Glioblastoma Using Single-Cell RNA Sequencing Datasets ', Cancers, vol. 15, no. 5, 1557 . https://doi.org/10.3390/cancers15051557
Cancers
Volume 15
Issue 5
Pages: 1557
Cancers
Volume 15
Issue 5
Pages: 1557
Targeting glioblastoma (GBM) stem-like cells (GSCs) is a common interest in both the laboratory investigation and clinical treatment of GBM. Most of the currently applied GBM stem-like markers lack validation and comparison with common standards rega
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f44d6831a563eacc7572efa0da3fcd76
https://doi.org/10.3390/cancers15051557
https://doi.org/10.3390/cancers15051557
Publikováno v:
Liisberg, M B, Lahtinen, S, Sloth, A B, Soukka, T & Vosch, T 2021, ' Frequency Encoding of Upconversion Nanoparticle Emission for Multiplexed Imaging of Spectrally and Spatially Overlapping Lanthanide Ions ', Journal of the American Chemical Society, vol. 143, no. 46, pp. 19399–19405 . https://doi.org/10.1021/jacs.1c07691
We present frequency encoded upconversion (FE-UPCON) widefield microscopy, an imaging approach that allows for multiplexed signal recovery based on frequency encoding of selected upconverted lanthanide ion emission rather than separation based on ene
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0da6ffba9541e019e8125239a8c9d350
https://curis.ku.dk/ws/files/333435639/jacs.1c07691.pdf
https://curis.ku.dk/ws/files/333435639/jacs.1c07691.pdf
Publikováno v:
International Journal of Molecular Sciences; Volume 23; Issue 6; Pages: 3308
Sell, D K, Sloth, A B, Bakhshinejad, B & Kjaer, A 2022, ' A White Plaque, Associated with Genomic Deletion, Derived from M13KE-Based Peptide Library Is Enriched in a Target-Unrelated Manner during Phage Display Biopanning Due to Propagation Advantage ', International Journal of Molecular Sciences, vol. 23, no. 6, 3308, pp. 1-16 . https://doi.org/10.3390/ijms23063308
Sell, D K, Sloth, A B, Bakhshinejad, B & Kjaer, A 2022, ' A White Plaque, Associated with Genomic Deletion, Derived from M13KE-Based Peptide Library Is Enriched in a Target-Unrelated Manner during Phage Display Biopanning Due to Propagation Advantage ', International Journal of Molecular Sciences, vol. 23, no. 6, 3308, pp. 1-16 . https://doi.org/10.3390/ijms23063308
The nonspecific enrichment of target-unrelated peptides during biopanning remains a major drawback for phage display technology. The commercial Ph.D.TM-7 phage display library is used extensively for peptide discovery. This library is based on the M1
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3a3bbf0b4158e6334f5d279e5390bd29
https://doi.org/10.3390/ijms23063308
https://doi.org/10.3390/ijms23063308