Zobrazeno 1 - 10
of 27
pro vyhledávání: '"Andrew V, Anzalone"'
Autor:
Jonathan Y. Hsu, Julian Grünewald, Regan Szalay, Justine Shih, Andrew V. Anzalone, Kin Chung Lam, Max W. Shen, Karl Petri, David R. Liu, J. Keith Joung, Luca Pinello
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-6 (2021)
Prime editing guide RNA design is more complex than for standard CRISPR-based nucleases or base editors. Here the authors present PrimeDesign and PrimeVar for the rapid and simplified design of pegRNA and ngRNA combinations.
Externí odkaz:
https://doaj.org/article/641f29fd5ac64f28bbea06a81c5212bd
Autor:
Zackery A. Ely, Nicolas Mathey-Andrews, Santiago Naranjo, Samuel I. Gould, Kim L. Mercer, Gregory A. Newby, Christina M. Cabana, William M. Rideout, Grissel Cervantes Jaramillo, Jennifer M. Khirallah, Katie Holland, Peyton B. Randolph, William A. Freed-Pastor, Jessie R. Davis, Zachary Kulstad, Peter M. K. Westcott, Lin Lin, Andrew V. Anzalone, Brendan L. Horton, Nimisha B. Pattada, Sean-Luc Shanahan, Zhongfeng Ye, Stefani Spranger, Qiaobing Xu, Francisco J. Sánchez-Rivera, David R. Liu, Tyler Jacks
Publikováno v:
Nature Biotechnology.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ae1a4aa0f1935d98be9c88b1dc7016f7
https://doi.org/10.1038/s41587-023-01783-y
https://doi.org/10.1038/s41587-023-01783-y
Autor:
Jessie R. Davis, Samagya Banskota, Jonathan M. Levy, Gregory A. Newby, Xiao Wang, Andrew V. Anzalone, Andrew T. Nelson, Peter J. Chen, Andrew D. Hennes, Meirui An, Heejin Roh, Peyton B. Randolph, Kiran Musunuru, David R. Liu
Publikováno v:
Nature Biotechnology.
Realizing the promise of prime editing for the study and treatment of genetic disorders requires efficient methods for delivering prime editors (PEs) in vivo. Here we describe the identification of bottlenecks limiting adeno-associated virus (AAV)-me
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d1c52b9af05a263092511313fbc5d3a5
https://doi.org/10.1038/s41587-023-01758-z
https://doi.org/10.1038/s41587-023-01758-z
Autor:
Andrew V. Anzalone, Xin D. Gao, Christopher J. Podracky, Andrew T. Nelson, Luke W. Koblan, Aditya Raguram, Jonathan M. Levy, Jaron A. M. Mercer, David R. Liu
Publikováno v:
Nature Biotechnology. 40:731-740
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::641f156ba3afd1b0de0090752d8e0a32
https://doi.org/10.1038/s41587-021-01133-w
https://doi.org/10.1038/s41587-021-01133-w
Autor:
Alvin Hsu, Andrew V. Anzalone, Peyton B. Randolph, James W. Nelson, Simon P. Shen, Kelcee A. Everette, Meirui An, Gregory A. Newby, David R. Liu, Peter J. Chen, Jonathan C. Chen
Publikováno v:
Nature Biotechnology. 40:402-410
Prime editing enables the installation of virtually any combination of point mutations, small insertions or small deletions in the DNA of living cells. A prime editing guide RNA (pegRNA) directs the prime editor protein to the targeted locus and also
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b10210353dfc4e217eed08131ecc552b
https://doi.org/10.1038/s41587-021-01039-7
https://doi.org/10.1038/s41587-021-01039-7
Autor:
Luke W. Koblan, Britt Adamson, Albert Xu, Mandana Arbab, Tyler A Sisley, Jordan L. Doman, Dian Yang, Joseph M. Replogle, David R. Liu, Andrew V. Anzalone, Jeffrey A. Hussmann, Max W. Shen, Gregory A. Newby, Beverly Mok, Jonathan S. Weissman
Publikováno v:
Nature Biotechnology. 39:1414-1425
Programmable C•G-to-G•C base editors (CGBEs) have broad scientific and therapeutic potential, but their editing outcomes have proved difficult to predict and their editing efficiency and product purity are often low. We describe a suite of engine
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::794631ca2dbb8219581743d613b4016c
https://doi.org/10.1038/s41587-021-00938-z
https://doi.org/10.1038/s41587-021-00938-z
Autor:
Luca Pinello, Max W. Shen, Regan Szalay, Julian Grünewald, Jonathan Y. Hsu, J. Keith Joung, Andrew V. Anzalone, Justine Shih, David R. Liu, Kin Chung Lam, Karl Petri
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-6 (2021)
Nature Communications
Nature Communications
Prime editing (PE) is a versatile genome editing technology, but design of the required guide RNAs is more complex than for standard CRISPR-based nucleases or base editors. Here we describe PrimeDesign, a user-friendly, end-to-end web application and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0ef65e9d4e85e74b4a85709efb6e1dc2
https://doaj.org/article/641f29fd5ac64f28bbea06a81c5212bd
https://doaj.org/article/641f29fd5ac64f28bbea06a81c5212bd
Publikováno v:
Nature Biotechnology. 38:824-844
The development of new CRISPR-Cas genome editing tools continues to drive major advances in the life sciences. Four classes of CRISPR-Cas-derived genome editing agents-nucleases, base editors, transposases/recombinases and prime editors-are currently
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9fc5914cb224d518024474a6e123bc85
https://doi.org/10.1038/s41587-020-0561-9
https://doi.org/10.1038/s41587-020-0561-9
Autor:
Yuan Zong, Shuai Jin, Shengxing Wang, Qiupeng Lin, David R. Liu, Aditya Raguram, Andrew V. Anzalone, Yanpeng Wang, Chenxiao Xue, Jordan L. Doman, Zixu Zhu, Caixia Gao
Publikováno v:
Nature Biotechnology. 38:582-585
Prime editors, which are CRISPR-Cas9 nickase (H840A)-reverse transcriptase fusions programmed with prime editing guide RNAs (pegRNAs), can edit bases in mammalian cells without donor DNA or double-strand breaks. We adapted prime editors for use in pl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e406778ec0c45d16fb84181348153a41
https://doi.org/10.1038/s41587-020-0455-x
https://doi.org/10.1038/s41587-020-0455-x
Autor:
Luke W. Koblan, Peter J. Chen, Alexander A. Sousa, Aditya Raguram, Peyton B. Randolph, Gregory A. Newby, Jonathan M. Levy, Andrew V. Anzalone, Christine D. Wilson, David R. Liu, Jessie Rose Davis
Publikováno v:
Nature
Most genetic variants that contribute to disease1 are challenging to correct efficiently and without excess byproducts2-5. Here we describe prime editing, a versatile and precise genome editing method that directly writes new genetic information into
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a8a837c29b98029c95efdb2491661af0
https://doi.org/10.1038/s41586-019-1711-4
https://doi.org/10.1038/s41586-019-1711-4