Zobrazeno 1 - 10
of 27
pro vyhledávání: '"Andrew Santiago-Frangos"'
Autor:
Dominick R Faith, Margie Kinnersley, Diane M Brooks, Dan Drecktrah, Laura S Hall, Eric Luo, Andrew Santiago-Frangos, Jenny Wachter, D Scott Samuels, Patrick R Secor
Publikováno v:
PLoS Pathogens, Vol 20, Iss 4, p e1012122 (2024)
Lyme disease is a tick-borne infection caused by the spirochete Borrelia (Borreliella) burgdorferi. Borrelia species have highly fragmented genomes composed of a linear chromosome and a constellation of linear and circular plasmids some of which are
Externí odkaz:
https://doaj.org/article/520a948c3726420084c82562d7c08bc7
Autor:
Anna Nemudraia, Artem Nemudryi, Murat Buyukyoruk, Andrew M. Scherffius, Trevor Zahl, Tanner Wiegand, Shishir Pandey, Joseph E. Nichols, Laina N. Hall, Aidan McVey, Helen H. Lee, Royce A. Wilkinson, Laura R. Snyder, Joshua D. Jones, Kristin S. Koutmou, Andrew Santiago-Frangos, Blake Wiedenheft
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-12 (2022)
Many viral diagnostic approaches require nucleic acid extraction and amplification prior to detection. Here, the authors develop a method based on type III CRISPR systems which allows sequence specific capture, concentration, and detection of viral R
Externí odkaz:
https://doaj.org/article/ec88a12a41b642dfb6cdf759b457932d
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-11 (2022)
Bacterial sRNAs compete for limited copies of an RNA chaperone. Here, real-time single-molecule imaging shows that pairwise interactions of sRNAs with Hfq determine if the sRNAs are actively exchanged or stably coexist on the shared protein.
Externí odkaz:
https://doaj.org/article/69a2f5a1e13f4692b75a6d372c1092f8
Autor:
Marscha Hirschi, Wang-Ting Lu, Andrew Santiago-Frangos, Royce Wilkinson, Sarah M. Golden, Alan R. Davidson, Gabriel C. Lander, Blake Wiedenheft
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-6 (2020)
The anti-CRISPR protein IF9 (AcrIF9) specifically inhibits the type I-F CRISPR adaptive immune system. Here, the authors present the cryo-EM structure of AcrIF9 in complex with the type I-F CRISPR RNA-guided surveillance complex (Csy) and a dsDNA bou
Externí odkaz:
https://doaj.org/article/d3c21875e57643ea9c2043b059ad21af
Autor:
Andrew Santiago-Frangos, Laina N. Hall, Anna Nemudraia, Artem Nemudryi, Pushya Krishna, Tanner Wiegand, Royce A. Wilkinson, Deann T. Snyder, Jodi F. Hedges, Calvin Cicha, Helen H. Lee, Ava Graham, Mark A. Jutila, Matthew P. Taylor, Blake Wiedenheft
Publikováno v:
Cell Reports Medicine, Vol 2, Iss 6, Pp 100319- (2021)
Summary: There is an urgent need for inexpensive new technologies that enable fast, reliable, and scalable detection of viruses. Here, we repurpose the type III CRISPR-Cas system for sensitive and sequence-specific detection of SARS-CoV-2. RNA recogn
Externí odkaz:
https://doaj.org/article/2ce73f76be06423891b13d498e6cbb2f
Publikováno v:
eLife, Vol 6 (2017)
The RNA chaperone Hfq is an Sm protein that facilitates base pairing between bacterial small RNAs (sRNAs) and mRNAs involved in stress response and pathogenesis. Hfq possesses an intrinsically disordered C-terminal domain (CTD) that may tune the func
Externí odkaz:
https://doaj.org/article/3ddd8ecec9c64b9f8583d25b0ea4d650
Autor:
Tanner Wiegand, Royce Wilkinson, Andrew Santiago-Frangos, Mackenzie Lynes, Roland Hatzenpichler, Blake Wiedenheft
Publikováno v:
CRISPR J
Cas10 proteins are large subunits of type III CRISPR RNA (crRNA)-guided surveillance complexes, many of which have nuclease and cyclase activities. Here, we use computational and phylogenetic methods to identify and analyze 2014 Cas10 sequences from
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::866e4997d21db5db7972cf579deb3c23
https://doi.org/10.1089/crispr.2022.0085
https://doi.org/10.1089/crispr.2022.0085
Autor:
Andrew Santiago-Frangos, William S. Henriques, Tanner Wiegand, Colin C. Gauvin, Murat Buyukyoruk, Ava B. Graham, Royce A. Wilkinson, Lenny Triem, Kasahun Neselu, Edward T. Eng, Gabriel C. Lander, Blake Wiedenheft
Bacteria and archaea acquire resistance to viruses and plasmids by integrating fragments of foreign DNA into the first repeat of a CRISPR array. However, the mechanism of site-specific integration remains poorly understood. Here, we determine a 560 k
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::cbad8f011ac3553dbd07460f53e46c57
https://doi.org/10.1101/2023.05.26.542337
https://doi.org/10.1101/2023.05.26.542337
Autor:
Andrew Santiago-Frangos, William Henriques, Tanner Wiegand, Colin Gauvin, Murat Buyukyoruk, Kasahun Neselu, Edward Eng, Gabriel Lander, Blake Wiedenheft
Publikováno v:
Journal of Biological Chemistry. 299:103459
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::584cd6c57f82794e0cd73182ebc25614
https://doi.org/10.1016/j.jbc.2023.103459
https://doi.org/10.1016/j.jbc.2023.103459
Publikováno v:
Nature communications. 13(1)
Hundreds of bacterial small RNAs (sRNAs) require the Hfq chaperone to regulate mRNA expression. Hfq is limiting, thus competition among sRNAs for binding to Hfq shapes the proteomes of individual cells. To understand how sRNAs compete for a common pa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f92a11a190517cb5223b7c9638a0db1c
https://pubmed.ncbi.nlm.nih.gov/35508531
https://pubmed.ncbi.nlm.nih.gov/35508531