Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Andrew Leduc"'
Publikováno v:
Genome Biology, Vol 23, Iss 1, Pp 1-31 (2022)
Abstract Background Many biological processes, such as cell division cycle and drug resistance, are reflected in protein covariation across single cells. This covariation can be quantified and interpreted by single-cell mass spectrometry with suffici
Externí odkaz:
https://doaj.org/article/d160c19bb2534a74ada075541bdfd6d0
Autor:
R. Gray Huffman, Andrew Leduc, Christoph Wichmann, Marco Di Gioia, Francesco Borriello, Harrison Specht, Jason Derks, Saad Khan, Luke Khoury, Edward Emmott, Aleksandra A. Petelski, David H. Perlman, Jürgen Cox, Ivan Zanoni, Nikolai Slavov
Publikováno v:
Nature Methods. 20:714-722
Major aims of single-cell proteomics include increasing the consistency, sensitivity and depth of protein quantification, especially for proteins and modifications of biological interest. Here, to simultaneously advance all these aims, we developed p
Mass-spectrometry (MS) enables specific and accurate quantification of proteins with ever increasing throughput and sensitivity. Maximizing this potential of MS requires optimizing data acquisition parameters and performing efficient quality control
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6b83d2fd332b3129b37796b603eae2fe
https://doi.org/10.1101/2023.02.02.526809
https://doi.org/10.1101/2023.02.02.526809
Autor:
Laurent Gatto, Ruedi Aebersold, Juergen Cox, Vadim Demichev, Jason Derks, Edward Emmott, Alexander M. Franks, Alexander R. Ivanov, Ryan T. Kelly, Luke Khoury, Andrew Leduc, Michael J. MacCoss, Peter Nemes, David H. Perlman, Aleksandra A. Petelski, Christopher M. Rose, Erwin M. Schoof, Jennifer Van Eyk, Christophe Vanderaa, John R. Yates, Nikolai Slavov
Publikováno v:
Nature Methods, Vol. 20, no.3, p. 375-386 (2023)
Nat Methods
Nat Methods
Analyzing proteins from single cells by tandem mass spectrometry (MS) has become technically feasible. While such analysis has the potential to accurately quantify thousands of proteins across thousands of single cells, the accuracy and reproducibili
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8ffcf2355b99e372fc841c11cdcc6592
https://hdl.handle.net/2078.1/273466
https://hdl.handle.net/2078.1/273466
Autor:
Andrew Leduc
Protocol for preparing single cells for mass-spec analysis by nPOP as described by Leduc et al., 2021, 2022 DOI: 10.1186/s13059-022-02817-5. nPOP uses piezo acoustic dispensing to isolate individual cells in 300 picoliter volumes and performs all sub
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4b99039cdf5a8a922a3593d47635cc06
https://doi.org/10.17504/protocols.io.4r3l24r7qg1y/v3
https://doi.org/10.17504/protocols.io.4r3l24r7qg1y/v3
Autor:
R Gray Huffman, Andrew Leduc, Christoph Wichmann, Marco di Gioia, Francesco Borriello, Harrison Specht, Jason Derks, Saad Khan, Luke Khoury, Edward Emmott, Aleksandra A. Petelski, David H Perlman, Jürgen Cox, Ivan Zanoni, Nikolai Slavov
Major aims of single-cell proteomics include increasing the consistency, sensitivity, and depth of protein quantification, especially for proteins and modifications of biological interest. To simultaneously advance all these aims, we developed priori
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5fa6871c587c9af505a64fa41b0578c6
https://doi.org/10.1101/2022.03.16.484655
https://doi.org/10.1101/2022.03.16.484655
Autor:
Jason Derks, Andrew Leduc, Georg Wallmann, R. Gray Huffman, Matthew Willetts, Saad Khan, Harrison Specht, Markus Ralser, Vadim Demichev, Nikolai Slavov
Publikováno v:
Nature Biotechnology
Nat Biotechnol
Nat Biotechnol
Current mass-spectrometry methods enable high-throughput proteomics of large sample amounts, but proteomics of low sample amounts remains limited in depth and throughput. To increase the throughput of sensitive proteomics, we developed an experimenta
Autor:
R. Gray Huffman, Aleksandra A. Petelski, Andrew Leduc, Harrison Specht, Edward Emmott, David H. Perlman, Nikolai Slavov
Publikováno v:
Nature Protocols
Nat Protoc
Nat Protoc
Many biological systems are composed of diverse single cells. This diversity necessitates functional and molecular single-cell analysis. Single-cell protein analysis has long relied on affinity reagents, but emerging mass-spectrometry methods (either
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a48a61879c63746354df9dec3b721bc1
https://doi.org/10.1101/2021.03.12.435034
https://doi.org/10.1101/2021.03.12.435034
Autor:
Michelle S. Mazzeo, Andrew Leduc, Matthew D. Wehrman, Holly E. Callahan, Kelly M. Schultz, Mark Schumm
Publikováno v:
AIChE Journal. 64:3168-3176