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pro vyhledávání: '"Andrew J. Dowd"'
Autor:
Andrew J. Dowd
Publikováno v:
Methods in Molecular Biology ISBN: 9781493972678
Enzyme activity assay methods can be used to corroborate the results generated by Difference Gel Electrophoresis (DIGE) proteomic experiments. Two assay methods were chosen to demonstrate how this can be achieved. Assays for determining the activity
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7a9236d5d5356f2238109268b9c1b7a8
https://doi.org/10.1007/978-1-4939-7268-5_21
https://doi.org/10.1007/978-1-4939-7268-5_21
Autor:
Andrew J. Dowd
Publikováno v:
Methods in Molecular Biology ISBN: 9781493972678
Assays for measuring enzyme activity can be useful tools for proteomics applications. Enzyme testing can be performed to validate an experimental system prior to a Difference Gel Electrophoresis (DIGE) proteomic experiment and can also be utilized as
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f04721786ffb0089abce2e19568e69d5
https://doi.org/10.1007/978-1-4939-7268-5_20
https://doi.org/10.1007/978-1-4939-7268-5_20
Autor:
Dietmar Andreas Lang, Halina Rose Novak, Simone Antonio De Rose, Andrew J. Dowd, Sukriti Singh, Jennifer A. Littlechild
Publikováno v:
Catalysts; Volume 7; Issue 3; Pages: 91
Thermomyces lanouginosa lipase has been used to develop improved methods for carrier-free immobilization, the Cross-Linked Enzyme Aggregates (CLEAs), for its application in detergent products. An activator step has been introduced to the CLEAs prepar
Autor:
Mark J. I. Paine, Evangelia Morou, John Vontas, Andrew J. Dowd, Andrew Steven, Janet Hemingway, Nikolaos E. Labrou, Hanafy M. Ismail
Publikováno v:
International Journal of Environmental Analytical Chemistry. 90:922-933
Recombinant glutathione-S-transferases (GSTs) can be used as analytical tools for the development of simple insecticide quantification assays. This assay explores the ability of pyrethroids to promote inhibition of the GST-catalysing 1-chloro-2,4-din
Autor:
Evangelia Morou, Janet Hemingway, John Vontas, Andrew J. Dowd, Andrew Steven, Mark J. I. Paine
Publikováno v:
Enzyme and Microbial Technology. 45:164-168
The natural ability of the detoxification enzymes glutathione transferases (GSTs) to interact with xenobiotics can be used for the production of colorimetric assays. Detection is usually based on the inhibition of the GST-catalysed reaction, with det
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 1774:93-101
The superinvasive phenotype exhibited by paclitaxel-selected variants of an in vitro invasive clonal population of the human cancer cell line, MDA-MB-435S were examined using DIGE (Fluorescence 2-D Difference Gel Electrophoresis) and mass spectrometr
Autor:
Samuel Dadzie, Olga Mayans, Craig S. Wilding, Martin J. Donnelly, Adam M. Jenkins, Marc A. T. Muskavitch, Fang Lu, Sara N. Mitchell, Pelagie Boko, Daniel J. Rigden, David Weetman, Hilary Ranson, Mark J. I. Paine, Kimberly Regna, Luc Djogbenou, Andrew J. Dowd
Publikováno v:
PLoS ONE, Vol 9, Iss 3, p e92662 (2014)
PLoS ONE
PLoS ONE
The development of resistance to insecticides has become a classic exemplar of evolution occurring within human time scales. In this study we demonstrate how resistance to DDT in the major African malaria vector Anopheles gambiae is a result of both
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b81c3d51b768d945febd2bf15006a156
Autor:
John P. Dalton, Andrew J. Dowd, Paul J. Brindley, Ross I. Brinkworth, Christiana K. Verity, Ciaran P. Brady
Publikováno v:
Archives of Biochemistry and Biophysics. 380:46-55
Adult Schistosoma mansoni blood flukes express two discrete cysteine proteinases, SmCL1 and SmCL2, both of which are related to the cathepsin L-like enzymes of the C1 family of peptidases. Our previous phylogenetic analysis indicated that SmCL1 is mo
Publikováno v:
Scopus-Elsevier
Fasciola hepatica secretes proteolytic enzymes to aid it to penetrate and migrate through the host tissues. Two of these proteinases have been purified and shown to be cathepsin L-like, and are termed, CL1 (27.5 kD) and CL2 (29 kD). The immunogenicit
Publikováno v:
Molecular and Biochemical Parasitology. 88:163-174
Cathepsin L2 is a major cysteine proteinase secreted by adult Fasciola hepatica . The enzyme differs from other reported cathepsin Ls in that it can cleave peptide substrates that contain proline in the P 2 position. A cDNA was isolated from an expre