The use of streptavidin conjugates as immunoblot loading controls and mitochondrial markers for use with Dictyostelium discoideum

Autor: Andrew J. Davidson, Jason S. King, Robert H. Insall

Publikováno v: BioTechniques, Vol 55, Iss 1, Pp 39-41 (2013)

The loading controls used for quantitative immunoblotting of mammalian proteins are not appropriate for use with Dictyostelium discoideum. Actin levels, for example, change greatly during Dictyostelium development. In addition, Dictyostelium-specific

Externí odkaz: https://doaj.org/article/1065809b71b942248b720c4391b37d4d

Live cell tracking of macrophage efferocytosis during

Autor: Michael H, Raymond, Andrew J, Davidson, Yi, Shen, Daniel R, Tudor, Christopher D, Lucas, Sho, Morioka, Justin S A, Perry, Julia, Krapivkina, David, Perrais, Linus J, Schumacher, Robert E, Campbell, Will, Wood, Kodi S, Ravichandran

Publikováno v: Science (New York, N.Y.). 375(6585)

Apoptosis of cells and their subsequent removal through efferocytosis occurs in nearly all tissues during development, homeostasis, and disease. However, it has been difficult to track cell death and subsequent corpse removal in vivo. We developed a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::be72fce5199cc77243303d77c34f23e4
https://pubmed.ncbi.nlm.nih.gov/35271315

PTPN21/Pez is a novel and evolutionarily conserved key regulator of inflammation in vivo

Autor: Jennie S. Campbell, Henry Todd, David A. Lyons, Frederico S.L.M. Rodrigues, Abigail Elliot, Jason J Early, Yi Feng, Will Wood, Andrew J. Davidson

Publikováno v: Campbell, J S, Davidson, A J, Todd, H, Rodrigues, F S L M, Elliot, A M, Early, J J, Lyons, D A, Feng, Y & Wood, W 2020, ' PTPN21/Pez is a novel and evolutionarily conserved key regulator of inflammation in vivo ', Current Biology, vol. 31, pp. 1-9 . https://doi.org/10.1016/j.cub.2020.11.014
Current Biology

Summary Drosophila provides a powerful model in which to study inflammation in vivo, and previous studies have revealed many of the key signaling events critical for recruitment of immune cells to tissue damage. In the fly, wounding stimulates the ra

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a77da1c4630ddc861e09d142cc65a720
https://www.pure.ed.ac.uk/ws/files/186325721/1_s2.0_S0960982220316894_main.pdf

WASP restricts active rac to maintain cells' front-rear polarization

Autor: Peter A. Thomason, Clelia Amato, Laura M. Machesky, Andrew J. Davidson, Shehab Ismail, Robert H. Insall, Karthic Swaminathan

Publikováno v: Amato, C, Thomason, P A, Davidson, A J, Swaminathan, K, Ismail, S, Machesky, L M & Insall, R H 2019, ' WASP restricts active rac to maintain cells' front-rear polarization ', Current Biology, vol. 29, no. 24, pp. 4169-4182.e4 . https://doi.org/10.1016/j.cub.2019.10.036
Current Biology

Summary Efficient motility requires polarized cells, with pseudopods at the front and a retracting rear. Polarization is maintained by restricting the pseudopod catalyst, active Rac, to the front. Here, we show that the actin nucleation-promoting fac

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ff3e71dff1cd156f84ba7ef5d490c1e1
https://www.pure.ed.ac.uk/ws/files/139989163/Davidson_A_WASP_restricts....pdf

Phagocyte Responses to Cell Death in Flies

Autor: Andrew J. Davidson, Will Wood

Publikováno v: Cold Spring Harb Perspect Biol

Multicellular organisms are not created through cell proliferation alone. It is through cell death that an indefinite cellular mass is pared back to reveal its true form. Cells are also lost throughout life as part of homeostasis and through injury.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a46f56dd68a829dcd0e7a59068926e92
https://doi.org/10.1101/cshperspect.a036350