Zobrazeno 1 - 10
of 45
pro vyhledávání: '"Andrew F. Gardner"'
Autor:
Leonardo Betancurt-Anzola, Markel Martínez-Carranza, Marc Delarue, Kelly M. Zatopek, Andrew F. Gardner, Ludovic Sauguet
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-15 (2023)
Abstract Replicative DNA polymerases duplicate entire genomes at high fidelity. This feature is shared among the three domains of life and is facilitated by their dual polymerase and exonuclease activities. Family D replicative DNA polymerases (PolD)
Externí odkaz:
https://doaj.org/article/6fa735390f164ee1a07c249234ceecde
Autor:
Travis J. Sanders, Fahad Ullah, Alexandra M. Gehring, Brett W. Burkhart, Robert L. Vickerman, Sudili Fernando, Andrew F. Gardner, Asa Ben-Hur, Thomas J. Santangelo
Publikováno v:
Frontiers in Microbiology, Vol 12 (2021)
Histone proteins compact and organize DNA resulting in a dynamic chromatin architecture impacting DNA accessibility and ultimately gene expression. Eukaryotic chromatin landscapes are structured through histone protein variants, epigenetic marks, the
Externí odkaz:
https://doaj.org/article/8c3cc65a66e54d168eccae5a494f2230
Autor:
Kelly M. Zatopek, Brett W. Burkhart, Richard D. Morgan, Alexandra M. Gehring, Kristin A. Scott, Thomas J. Santangelo, Andrew F. Gardner
Publikováno v:
Frontiers in Microbiology, Vol 12 (2021)
Thermococcus kodakarensis (T. kodakarensis), a hyperthermophilic, genetically accessible model archaeon, encodes two putative restriction modification (R-M) defense systems, TkoI and TkoII. TkoI is encoded by TK1460 while TkoII is encoded by TK1158.
Externí odkaz:
https://doaj.org/article/4131e7ff9cca4b30a7096adf843e5798
Autor:
Jiangyu Yan, Thomas R. Beattie, Adriana L. Rojas, Kelly Schermerhorn, Tamzin Gristwood, Jonathan C. Trinidad, Sonja V. Albers, Pietro Roversi, Andrew F. Gardner, Nicola G. A. Abrescia, Stephen D. Bell
Publikováno v:
Nature Communications, Vol 8, Iss 1, Pp 1-15 (2017)
The current model for B-family DNA polymerases in archaea is one of single-subunit enzymes in contrast to the multi-subunit complexes in eukaryotes. Here the authors show that PolB1 fromSulfolobus solfataricusexists as a heterotrimeric complex in cel
Externí odkaz:
https://doaj.org/article/627c7d9ffa8c4d6aa1b8ab4ad90d0baa
Autor:
Andrew F. Gardner, Zvi Kelman
Publikováno v:
Frontiers in Molecular Biosciences, Vol 6 (2019)
Externí odkaz:
https://doaj.org/article/865d2e9e054947e995f7f6e2987ea15d
Autor:
Andrew F. Gardner, Kiserian M. Jackson, Madeleine M. Boyle, Jackson A. Buss, Vladimir Potapov, Alexandra M. Gehring, Kelly M. Zatopek, Ivan R. Corrêa Jr., Jennifer L. Ong, William E. Jack
Publikováno v:
Frontiers in Molecular Biosciences, Vol 6 (2019)
A variant of 9°N DNA polymerase [Genbank ID (AAA88769.1)] with three mutations (D141A, E143A, A485L) and commercialized under the name “Therminator DNA polymerase” has the ability to incorporate a variety of modified nucleotide classes. This Rev
Externí odkaz:
https://doaj.org/article/dbab20a8f931428eabdea0cacf437dd8
Autor:
Guy Miezner, Israela Turgeman-Grott, Kelly M Zatopek, Andrew F Gardner, Leah Reshef, Deepak K Choudhary, Martina Alstetter, Thorsten Allers, Anita Marchfelder, Uri Gophna
Publikováno v:
microLife. 4
CRISPR-Cas systems provide heritable acquired immunity against viruses to archaea and bacteria. Cas3 is a CRISPR-associated protein that is common to all Type I systems, possesses both nuclease and helicase activities, and is responsible for degradat
Publikováno v:
Current Protocols. 2
The formation and persistence of DNA damage can impact biological processes such as DNA replication and transcription. To maintain genome stability and integrity, organisms rely on robust DNA damage repair pathways. Techniques to detect and locate DN
Autor:
Gregory J. S. Lohman, Léa Chuzel, Paul J Caffrey, Samantha L. Fossa, Andrew F. Gardner, Christopher H. Taron, Kelly M Zatopek, Katharina Bilotti, Alexandra M. Gehring
Publikováno v:
Applied and Environmental Microbiology. 88
It has been predicted that 30-80% of archaeal genomes remain annotated as hypothetical proteins with no assigned gene function. Further, many archaeal organisms are difficult to grow or are unculturable. To overcome these technical and experimental h