Zobrazeno 1 - 10
of 75
pro vyhledávání: '"Andrew F Gardner"'
Autor:
Leonardo Betancurt-Anzola, Markel Martínez-Carranza, Marc Delarue, Kelly M. Zatopek, Andrew F. Gardner, Ludovic Sauguet
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-15 (2023)
Abstract Replicative DNA polymerases duplicate entire genomes at high fidelity. This feature is shared among the three domains of life and is facilitated by their dual polymerase and exonuclease activities. Family D replicative DNA polymerases (PolD)
Externí odkaz:
https://doaj.org/article/6fa735390f164ee1a07c249234ceecde
Publikováno v:
PLoS ONE, Vol 19, Iss 12, p e0312743 (2024)
T7 RNA Polymerase (RNAP) is a widely used enzyme with recent applications in the production of RNA vaccines. For over 50 years denaturing sequencing gels have been used as key analysis tools for probing the nucleotide addition mechanisms of T7 RNAP a
Externí odkaz:
https://doaj.org/article/c2176722597144369060404416d7f971
Autor:
Travis J. Sanders, Fahad Ullah, Alexandra M. Gehring, Brett W. Burkhart, Robert L. Vickerman, Sudili Fernando, Andrew F. Gardner, Asa Ben-Hur, Thomas J. Santangelo
Publikováno v:
Frontiers in Microbiology, Vol 12 (2021)
Histone proteins compact and organize DNA resulting in a dynamic chromatin architecture impacting DNA accessibility and ultimately gene expression. Eukaryotic chromatin landscapes are structured through histone protein variants, epigenetic marks, the
Externí odkaz:
https://doaj.org/article/8c3cc65a66e54d168eccae5a494f2230
Autor:
Kelly M. Zatopek, Brett W. Burkhart, Richard D. Morgan, Alexandra M. Gehring, Kristin A. Scott, Thomas J. Santangelo, Andrew F. Gardner
Publikováno v:
Frontiers in Microbiology, Vol 12 (2021)
Thermococcus kodakarensis (T. kodakarensis), a hyperthermophilic, genetically accessible model archaeon, encodes two putative restriction modification (R-M) defense systems, TkoI and TkoII. TkoI is encoded by TK1460 while TkoII is encoded by TK1158.
Externí odkaz:
https://doaj.org/article/4131e7ff9cca4b30a7096adf843e5798
Autor:
Jiangyu Yan, Thomas R. Beattie, Adriana L. Rojas, Kelly Schermerhorn, Tamzin Gristwood, Jonathan C. Trinidad, Sonja V. Albers, Pietro Roversi, Andrew F. Gardner, Nicola G. A. Abrescia, Stephen D. Bell
Publikováno v:
Nature Communications, Vol 8, Iss 1, Pp 1-15 (2017)
The current model for B-family DNA polymerases in archaea is one of single-subunit enzymes in contrast to the multi-subunit complexes in eukaryotes. Here the authors show that PolB1 fromSulfolobus solfataricusexists as a heterotrimeric complex in cel
Externí odkaz:
https://doaj.org/article/627c7d9ffa8c4d6aa1b8ab4ad90d0baa
Autor:
Andrew F. Gardner, Zvi Kelman
Publikováno v:
Frontiers in Molecular Biosciences, Vol 6 (2019)
Externí odkaz:
https://doaj.org/article/865d2e9e054947e995f7f6e2987ea15d
Autor:
Andrew F. Gardner, Kiserian M. Jackson, Madeleine M. Boyle, Jackson A. Buss, Vladimir Potapov, Alexandra M. Gehring, Kelly M. Zatopek, Ivan R. Corrêa Jr., Jennifer L. Ong, William E. Jack
Publikováno v:
Frontiers in Molecular Biosciences, Vol 6 (2019)
A variant of 9°N DNA polymerase [Genbank ID (AAA88769.1)] with three mutations (D141A, E143A, A485L) and commercialized under the name “Therminator DNA polymerase” has the ability to incorporate a variety of modified nucleotide classes. This Rev
Externí odkaz:
https://doaj.org/article/dbab20a8f931428eabdea0cacf437dd8
Autor:
Guy Miezner, Israela Turgeman-Grott, Kelly M Zatopek, Andrew F Gardner, Leah Reshef, Deepak K Choudhary, Martina Alstetter, Thorsten Allers, Anita Marchfelder, Uri Gophna
Publikováno v:
microLife. 4
CRISPR-Cas systems provide heritable acquired immunity against viruses to archaea and bacteria. Cas3 is a CRISPR-associated protein that is common to all Type I systems, possesses both nuclease and helicase activities, and is responsible for degradat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e1f4b268a5f51bc572ba53569e2b62bb
https://doi.org/10.1093/femsml/uqad007
https://doi.org/10.1093/femsml/uqad007
Publikováno v:
Current Protocols. 2
The formation and persistence of DNA damage can impact biological processes such as DNA replication and transcription. To maintain genome stability and integrity, organisms rely on robust DNA damage repair pathways. Techniques to detect and locate DN
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5e8f524061699e6a6b8b2575c0d4f606
https://doi.org/10.1002/cpz1.595
https://doi.org/10.1002/cpz1.595