Zobrazeno 1 - 10
of 33
pro vyhledávání: '"Andrew D. Garst"'
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-9 (2020)
Bacteria lack the same suite of CRISPR tools that have been developed for mammalian cells. Here, the authors link aptamers to sgRNAs to allow small molecule control of gene editing in E. coli.
Externí odkaz:
https://doaj.org/article/ded3f93171cd4f4bb57d5a441ed60ade
Autor:
Cong Chen, Alaksh Choudhury, Shuanghong Zhang, Andrew D. Garst, Xin Song, Xunli Liu, Tao Chen, Ryan T. Gill, Zhiwen Wang
Publikováno v:
mSystems, Vol 5, Iss 2 (2020)
ABSTRACT It is important to expedite our understanding of antibiotic resistance to address the increasing numbers of fatalities and environmental pollution due to the emergence of antibiotic resistance and multidrug-resistant strains. Here, we combin
Externí odkaz:
https://doaj.org/article/8e0568820c9d484880741007f3b5bb0e
Autor:
Marcelo C Bassalo, Andrew D Garst, Alaksh Choudhury, William C Grau, Eun J Oh, Eileen Spindler, Tanya Lipscomb, Ryan T Gill
Publikováno v:
Molecular Systems Biology, Vol 14, Iss 11, Pp 1-15 (2018)
Abstract Our limited ability to predict genotype–phenotype relationships has called for strategies that allow testing of thousands of hypotheses in parallel. Deep scanning mutagenesis has been successfully implemented to map genotype–phenotype re
Externí odkaz:
https://doaj.org/article/24fb12df9e214e838712ba1d265ed489
Autor:
Z. Y. Liu, Emily F. Freed, Eun Joong Oh, Andrew D. Garst, Rongming Liu, Carrie Eckert, H. Chang, Liya Liang, Ryan T. Gill
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-11 (2019)
Liu, R M, Liang, L L, Freed, E, Chang, H, Oh, E, Liu, Z Y, Garst, A, Eckert, C A & Gill, R T 2019, ' Synthetic chimeric nucleases function for efficient genome editing ', Nature Communications, vol. 10, no. 1, 5524 . https://doi.org/10.1038/s41467-019-13500-y
Nature Communications
Liu, R M, Liang, L L, Freed, E, Chang, H, Oh, E, Liu, Z Y, Garst, A, Eckert, C A & Gill, R T 2019, ' Synthetic chimeric nucleases function for efficient genome editing ', Nature Communications, vol. 10, no. 1, 5524 . https://doi.org/10.1038/s41467-019-13500-y
Nature Communications
CRISPR–Cas systems have revolutionized genome editing across a broad range of biotechnological endeavors. Many CRISPR-Cas nucleases have been identified and engineered for improved capabilities. Given the modular structure of such enzymes, we hypot
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::df572759a9c3de0acdeb3dfeded031d9
http://link.springer.com/article/10.1038/s41467-019-13500-y
http://link.springer.com/article/10.1038/s41467-019-13500-y
Autor:
Tyson R. Shepherd, Clint Davis, Charles D. Johnson, Stephen Tanner, Jocelyne Bruand, Thomas Hraha, Tian Tian, Steven Alvarez, Eric Abbate, Shea Lambert, Timothy M. Shaver, Bryan A Leland, Paul Hardenbol, Michael Graige, Stephen Federowicz, Mathew Barber, Scott Bolte, Christopher G. Abraham, Miles Gander, Simon Cawley, Gozde Gencer, J. Christian J. Ray, Andrew D. Garst, Richard Fox, Maciej Swat, Surbhi Jain, Matthew Estes, Kara Juneau, Craig A. Struble, Ken Wishart, Andrea L. Halweg-Edwards, Deanna M. Church, Francesca Pearson, Eileen Colie Spindler, Nandini Krishnamurthy, Chad Sanada, Joshua Shorenstein
Genome engineering methodologies are transforming biological research and discovery. Approaches based on CRISPR technology have been broadly adopted and there is growing interest in the generation of massively parallel edited cell libraries. Comparin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3fe0e8bc1ff7b70e400f9a7f173cf58a
https://doi.org/10.1101/2021.09.23.458228
https://doi.org/10.1101/2021.09.23.458228
Autor:
Ryan T. Gill, Andrew D. Garst, Xin Song, Cong Chen, Zhiwen Wang, Tao Chen, Xunli Liu, Alaksh Choudhury, Shuanghong Zhang
Publikováno v:
mSystems, Vol 5, Iss 2, p e00232-20 (2020)
mSystems, Vol 5, Iss 2 (2020)
mSystems
mSystems, Vol 5, Iss 2 (2020)
mSystems
The growing threat of antimicrobial resistance poses a serious threat to public health care and motivates efforts to understand the means by which resistance acquisition occurs and how this can be combatted. To address these challenges, we expedited
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::54a9e4cabc1ebf996fdbcdf20750acc1
https://doaj.org/article/03bb703981c347c58b27cd5905800818
https://doaj.org/article/03bb703981c347c58b27cd5905800818
Publikováno v:
Nature Communications
Nature Communications, 2020, 11 (1), pp.1394. ⟨10.1038/s41467-020-15226-8⟩
Nature Communications, Vol 11, Iss 1, Pp 1-9 (2020)
Nature Communications, Nature Publishing Group, 2020, 11 (1), pp.1394. ⟨10.1038/s41467-020-15226-8⟩
Nature Communications, 2020, 11 (1), pp.1394. ⟨10.1038/s41467-020-15226-8⟩
Nature Communications, Vol 11, Iss 1, Pp 1-9 (2020)
Nature Communications, Nature Publishing Group, 2020, 11 (1), pp.1394. ⟨10.1038/s41467-020-15226-8⟩
CRISPR-Cas9 has led to great advances in gene editing for a broad spectrum of applications. To further the utility of Cas9 there have been efforts to achieve temporal control over its nuclease activity. While different approaches have focused on regu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6c9a57c27b85b736f4fe6155e3c7d002
https://doi.org/10.1038/s41467-020-15226-8
https://doi.org/10.1038/s41467-020-15226-8
Autor:
Ryan T. Gill, Eun Joong Oh, Gur Pines, Reilly G. Fankhauser, Carrie Eckert, Alaksh Choudhury, Marcelo C. Bassalo, Andrew D. Garst
Publikováno v:
ACS Synthetic Biology. 7:2824-2832
Sequence to activity mapping technologies are rapidly developing, enabling the generation and isolation of mutations conferring novel phenotypes. Here we used the CRISPR EnAbled Trackable genome Engineering (CREATE) technology to investigate the inhi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::96ff26622fa12b92df46616859355bb4
https://doi.org/10.1021/acssynbio.8b00219
https://doi.org/10.1021/acssynbio.8b00219
Autor:
Violeta Sànchez i Nogué, Ryan T. Gill, Gregg T. Beckham, Rongming Liu, Thomas Lee, Andrew D. Garst, Liya Liang
Publikováno v:
Metabolic Engineering. 41:1-10
Isopropanol is an important target molecule for sustainable production of fuels and chemicals. Increases in DNA synthesis and synthetic biology capabilities have resulted in the development of a range of new strategies for the more rapid design, cons
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::646436aca1cd61c2d4a389b6e9141a11
https://doi.org/10.1016/j.ymben.2017.02.009
https://doi.org/10.1016/j.ymben.2017.02.009
Autor:
Tanya E. W. Lipscomb, Alaksh Choudhury, Eileen Colie Spindler, Marcelo C. Bassalo, Andrew D. Garst, William C Grau, Eun J Oh, Ryan T. Gill
Publikováno v:
Molecular Systems Biology. 14
Our limited ability to predict genotype-phenotype relationships has called for strategies that allow testing of thousands of hypotheses in parallel. Deep scanning mutagenesis has been successfully implemented to map genotype-phenotype relationships a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::13d89b3da0c58f7f65c2126f0c8cb9d0
https://doi.org/10.15252/msb.20188371
https://doi.org/10.15252/msb.20188371