Zobrazeno 1 - 10
of 21
pro vyhledávání: '"Andrew C. Adey"'
Autor:
Sonia N. Acharya, Ruth V. Nichols, Lauren E. Rylaarsdam, Brendan L. O’Connell, Theodore P. Braun, Andrew C. Adey
Publikováno v:
Genome Biology, Vol 25, Iss 1, Pp 1-20 (2024)
Abstract DNA methylation is a key component of the mammalian epigenome, playing a regulatory role in development, disease, and other processes. Robust, high-throughput single-cell DNA methylation assays are now possible (sciMET); however, the genome-
Externí odkaz:
https://doaj.org/article/4bb047f9ed7a44c9bff98bb3ee0e3935
Autor:
Çiğdem Ak, Zeynep Sayar, Guillaume Thibault, Erik A. Burlingame, M.J. Kuykendall, Jennifer Eng, Alex Chitsazan, Koei Chin, Andrew C. Adey, Christopher Boniface, Paul T. Spellman, George V. Thomas, Ryan P. Kopp, Emek Demir, Young Hwan Chang, Vasilis Stavrinides, Sebnem Ece Eksi
Publikováno v:
iScience, Vol 27, Iss 9, Pp 110668- (2024)
Summary: Mapping the spatial interactions of cancer, immune, and stromal cell states presents novel opportunities for patient stratification and for advancing immunotherapy. While single-cell studies revealed significant molecular heterogeneity in pr
Externí odkaz:
https://doaj.org/article/af29b6f146c548339d8565cc10e61ec6
Autor:
Hao Zhang, Ryan M. Mulqueen, Natalie Iannuzo, Dominique O. Farrera, Francesca Polverino, James J. Galligan, Julie G. Ledford, Andrew C. Adey, Darren A. Cusanovich
Publikováno v:
Genome Biology, Vol 25, Iss 1, Pp 1-41 (2024)
Abstract We develop a large-scale single-cell ATAC-seq method by combining Tn5-based pre-indexing with 10× Genomics barcoding, enabling the indexing of up to 200,000 nuclei across multiple samples in a single reaction. We profile 449,953 nuclei acro
Externí odkaz:
https://doaj.org/article/45dac0a06c854b82a3eca3eae9b2d359
Autor:
Konstantin Queitsch, Travis W. Moore, Brendan L. O’Connell, Ruth V. Nichols, John L. Muschler, Dove Keith, Charles Lopez, Rosalie C. Sears, Gordon B. Mills, Galip Gürkan Yardımcı, Andrew C. Adey
Publikováno v:
Cell Reports: Methods, Vol 3, Iss 11, Pp 100625- (2023)
Summary: Single-cell whole-genome sequencing (scWGS) enables the assessment of genome-level molecular differences between individual cells with particular relevance to genetically diverse systems like solid tumors. The application of scWGS was limite
Externí odkaz:
https://doaj.org/article/50b503e5febd4b248aa5f55f696332a6
Autor:
Andrew C. Adey, PhD
Publikováno v:
Cell Reports: Methods, Vol 3, Iss 11, Pp 100648- (2023)
In this Q&A, Scientific Editor Emily Marcinkevicius talks with author Andrew Adey about developing a more broadly accessible method for paired whole-genome and chromatin accessibility sequencing from single cells, as well as the current and future la
Externí odkaz:
https://doaj.org/article/fce596debe6b4f11a2755446ef009a2b
Autor:
Ruth V. Nichols, Brendan L. O’Connell, Ryan M. Mulqueen, Jerushah Thomas, Ashley R. Woodfin, Sonia Acharya, Gail Mandel, Dmitry Pokholok, Frank J. Steemers, Andrew C. Adey
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-10 (2022)
Despite the importance of DNA methylation, accessible and high-throughput methods to profile methylation at the single-cell level are lacking. Here, the authors present sciMETv2, a high-throughput workflow that provides high-quality single-cell methy
Externí odkaz:
https://doaj.org/article/819c0be9d5a24f45850610d10e5f6cef
Autor:
Sebnem Ece Eksi, Alex Chitsazan, Zeynep Sayar, George V. Thomas, Andrew J. Fields, Ryan P. Kopp, Paul T. Spellman, Andrew C. Adey
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-14 (2021)
High tumour heterogeneity hinders the identification of molecular subtypes in prostate cancer. Here, the authors integrate single-cell chromatin accessibility data with multiplex imaging and reveal distinct chromatin features and transcriptional fact
Externí odkaz:
https://doaj.org/article/9f8c75a4a6604f2d9bd47a929b95d244
Autor:
Timothy A. Bates, Hans C. Leier, Zoe L. Lyski, Savannah K. McBride, Felicity J. Coulter, Jules B. Weinstein, James R. Goodman, Zhengchun Lu, Sarah A. R. Siegel, Peter Sullivan, Matt Strnad, Amanda E. Brunton, David X. Lee, Andrew C. Adey, Benjamin N. Bimber, Brian J. O’Roak, Marcel E. Curlin, William B. Messer, Fikadu G. Tafesse
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-7 (2021)
Here, the authors show that neutralization of human sera from both BNT162b2 vaccine recipients and from convalescent COVID-19 patients is less efficient against SARS- CoV-2 variants B.1.1.7 and B.1.351 and negatively associated with patient age.
Externí odkaz:
https://doaj.org/article/bb8db59a951140a893c150893245d2b7
Autor:
Casey A. Thornton, Ryan M. Mulqueen, Kristof A. Torkenczy, Andrew Nishida, Eve G. Lowenstein, Andrew J. Fields, Frank J. Steemers, Wenri Zhang, Heather L. McConnell, Randy L. Woltjer, Anusha Mishra, Kevin M. Wright, Andrew C. Adey
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-16 (2021)
Spatial orientation of cells in an interconnected network is lost in high-throughput single-cell epigenomic assays. Here the authors present sciMAP-ATAC to produce spatially resolved single-cell ATAC-seq data.
Externí odkaz:
https://doaj.org/article/1ad12e0e2a2a4ba0b41d734d7e7f4e32
Autor:
Brendan L. O'Connell, Ruth V. Nichols, Dmitry Pokholok, Jerushah Thomas, Sonia N. Acharya, Andrew Nishida, Casey A. Thornton, Marissa Co, Andrew J. Fields, Frank J. Steemers, Andrew C. Adey
Publikováno v:
Genome Research. 33:208-217
Here we present advancements in single-cell combinatorial indexed Assay for Transposase Accessible Chromatin (sciATAC) to measure chromatin accessibility that leverage nanowell chips to achieve atlas-scale cell throughput (>105cells) at low cost. The
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::df503b648ae2345ba7ffa2ea69f00081
https://doi.org/10.1101/gr.276655.122
https://doi.org/10.1101/gr.276655.122