Zobrazeno 1 - 10
of 11
pro vyhledávání: '"Andrew B Morgenthaler"'
Autor:
Andrew B Morgenthaler, Wallis R Kinney, Christopher C Ebmeier, Corinne M Walsh, Daniel J Snyder, Vaughn S Cooper, William M Old, Shelley D Copley
Publikováno v:
eLife, Vol 8 (2019)
New enzymes often evolve by gene amplification and divergence. Previous experimental studies have followed the evolutionary trajectory of an amplified gene, but have not considered mutations elsewhere in the genome when fitness is limited by an evolv
Externí odkaz:
https://doaj.org/article/4eadcc7a4c55458a92354cb207467c04
Autor:
JohnCarlo Kristofich, Andrew B Morgenthaler, Wallis R Kinney, Christopher C Ebmeier, Daniel J Snyder, William M Old, Vaughn S Cooper, Shelley D Copley
Publikováno v:
PLoS Genetics, Vol 14, Iss 8, p e1007615 (2018)
Synonymous mutations do not alter the specified amino acid but may alter the structure or function of an mRNA in ways that impact fitness. There are few examples in the literature, however, in which the effects of synonymous mutations on microbial gr
Externí odkaz:
https://doaj.org/article/13324c5830f64e058f7351d732925e53
Autor:
Ryan T. Gill, Alaksh Choudhury, Reilly G. Fankhauser, Emily F. Freed, Eun Joong Oh, Andrew B. Morgenthaler, Marcelo C. Bassalo, Shelley D. Copley, Joel L. Kaar
Publikováno v:
Choudhury, A, Fankhauser, R G, Freed, E F, Oh, E J, Morgenthaler, A B, Bassalo, M C, Copley, S D, Kaar, J L & Gill, R T 2020, ' Determinants for efficient editing with cas9-mediated recombineering in Escherichia coli ', ACS Synthetic Biology, vol. 9, no. 5, pp. 1083-1099 . https://doi.org/10.1021/acssynbio.9b00440
In E. coli, editing efficiency with Cas9-mediated recombineering varies across targets due to differences in the level of Cas9:gRNA-mediated DNA double-strand break (DSB)-induced cell death. We found that editing efficiency with the same gRNA and rep
Publikováno v:
Molecular Biology and Evolution
New enzymes often evolve by duplication and divergence of genes encoding enzymes with promiscuous activities that have become important in the face of environmental opportunities or challenges. Amplifications that increase the copy number of the gene
Autor:
Alaksh, Choudhury, Reilly G, Fankhauser, Emily F, Freed, Eun Joong, Oh, Andrew B, Morgenthaler, Marcelo C, Bassalo, Shelley D, Copley, Joel L, Kaar, Ryan T, Gill
Publikováno v:
ACS synthetic biology. 9(5)
In
Autor:
Vaughn S. Cooper, Christopher C. Ebmeier, Andrew B. Morgenthaler, William M. Old, Shelley D. Copley, Daniel J. Snyder, Wallis R. Kinney, Corinne M. Walsh
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::dc713c4bfd2cc719598aef24a596ca74
https://doi.org/10.7554/elife.53535.sa2
https://doi.org/10.7554/elife.53535.sa2
Autor:
William M. Old, Wallis R. Kinney, Vaughn S. Cooper, Shelley D. Copley, Corinne M. Walsh, Christopher C. Ebmeier, Daniel J. Snyder, Andrew B. Morgenthaler
Publikováno v:
eLife, Vol 8 (2019)
eLife
eLife
New enzymes often evolve by gene amplification and divergence. Previous experimental studies have followed the evolutionary trajectory of an amplified gene, but have not considered mutations elsewhere in the genome when fitness is limited by an evolv
Autor:
Corinne M. Walsh, William M. Old, Vaughn S. Cooper, Christopher C. Ebmeier, Daniel J. Snyder, Shelley D. Copley, Wallis R. Kinney, Andrew B. Morgenthaler
New enzymes often evolve by amplification and divergence of genes encoding enzymes with a weak ability to provide a new function. Experimental studies to date have followed the evolutionary trajectory of an amplified gene, but have not addressed othe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::706754774e688153155e604f3f5625e9
https://doi.org/10.1101/624205
https://doi.org/10.1101/624205
Autor:
Andrew B. Morgenthaler, Tobias Fuhrer, Jake J. Flood, Cyrus Gidfar, Vaughn S. Cooper, Michael R. Kristofich, Christopher C. Ebmeier, Daniel J. Snyder, Juhan Kim, William M. Old, Uwe Sauer, Shelley D. Copley
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America
Proceedings of the National Academy of Sciences of the United States of America, 116 (48)
Proceedings of the National Academy of Sciences of the United States of America, 116 (48)
PdxB (erythronate 4-phosphate dehydrogenase) is expected to be required for synthesis of the essential cofactor pyridoxal 5′-phosphate (PLP) in Escherichia coli. Surprisingly, incubation of the ∆pdxB strain in medium containing glucose as a sole
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cac8de85e873c6ad3dea02efbf97443f
Autor:
Jamie P. Kershner, Christopher C. Ebmeier, Andrew B. Morgenthaler, Sean Yu McLoughlin, Juhan Kim, William M. Old, Shelley D. Copley
Publikováno v:
Journal of bacteriology. 198(20)
When microbes are faced with an environmental challenge or opportunity, preexisting enzymes with promiscuous secondary activities can be recruited to provide newly important functions. Mutations that increase the efficiency of a new activity often co