PI 3 kinase related kinases-independent proteolysis of BRCA1 regulates Rad51 recruitment during genotoxic stress in human cells.

Autor: Ian Hammond-Martel, Helen Pak, Helen Yu, Raphael Rouget, Andrew A Horwitz, Jeffrey D Parvin, Elliot A Drobetsky, El Bachir Affar

Publikováno v: PLoS ONE, Vol 5, Iss 11, p e14027 (2010)

The function of BRCA1 in response to ionizing radiation, which directly generates DNA double strand breaks, has been extensively characterized. However previous investigations have produced conflicting data on mutagens that initially induce other cla

Externí odkaz: https://doaj.org/article/71ebaf2397b44b62b44bdde2c71e36cc

Supplementary Figure 1 from BRCA1 Represses Amphiregulin Gene Expression

Autor: Jeffrey D. Parvin, Andrew A. Horwitz, Ekaterina P. Lamber

Supplementary Figure 1 from BRCA1 Represses Amphiregulin Gene Expression

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e11a2ba7cb2d37421766b12071fdf69
https://doi.org/10.1158/0008-5472.22386048.v1

Supplementary Figure 3 from BRCA1 Represses Amphiregulin Gene Expression

Autor: Jeffrey D. Parvin, Andrew A. Horwitz, Ekaterina P. Lamber

Supplementary Figure 3 from BRCA1 Represses Amphiregulin Gene Expression

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::615ac7d465a37cd25fe76e655dffb7d7
https://doi.org/10.1158/0008-5472.22386042.v1

Method for Multiplexed Integration of Synergistic Alleles and Metabolic Pathways in Yeasts via CRISPR-Cas9

Autor: Kristy Michelle Hawkins, Darren Platt, Max G. Schubert, Sunil S. Chandran, Andrew A. Horwitz, Jack D. Newman, Tina Mahatdejkul-Meadows, Jessica M. Walter, Stephanie H. Kung, Aaron D. Hernday, Wayne Szeto

Publikováno v: Methods in Molecular Biology ISBN: 9781493997350

CRISPR-Cas has proven to be a powerful tool for precision genetic engineering in a variety of difficult genetic systems. In the highly tractable yeast S. cerevisiae, CRISPR-Cas can be used to conduct multiple engineering steps in parallel, allowing f

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::ad30205bac9a685c020b058739f269cf
https://doi.org/10.1007/978-1-4939-9736-7_3

Efficient Multiplexed Integration of Synergistic Alleles and Metabolic Pathways in Yeasts via CRISPR-Cas

Autor: Aaron D. Hernday, Sunil S. Chandran, Stephanie H. Kung, Wayne Szeto, Jessica M. Walter, Max G. Schubert, Tina Mahatdejkul-Meadows, Kristy Michelle Hawkins, Darren Platt, Jack D. Newman, Andrew A. Horwitz

Publikováno v: Horwitz, AA; Walter, JM; Schubert, MG; Kung, SH; Hawkins, K; Platt, DM; et al.(2015). Efficient Multiplexed Integration of Synergistic Alleles and Metabolic Pathways in Yeasts via CRISPR-Cas. Cell Systems, 1(1), 88-96. doi: 10.1016/j.cels.2015.02.001. UC Merced: Retrieved from: http://www.escholarship.org/uc/item/01d6s8m1
Cell systems, vol 1, iss 1

© 2015 The Authors. Summary CRISPR-Cas genome engineering in yeast has relied on preparation of complex expression plasmids for multiplexed gene knockouts and point mutations. Here we show that co-transformation of a single linearized plasmid with m

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d27ed41b04db0f82fc173913678fa9e8
https://doi.org/10.1016/j.cels.2015.02.001