Zobrazeno 1 - 10
of 137
pro vyhledávání: '"Andrei G Pakhomov"'
Publikováno v:
PLoS ONE, Vol 16, Iss 9, p e0257287 (2021)
AimsRecently, a new defibrillation modality using nanosecond pulses was shown to be effective at much lower energies than conventional 10 millisecond monophasic shocks in ex vivo experiments. Here we compare the safety factors of 300 nanosecond and 1
Externí odkaz:
https://doaj.org/article/02ce03bdcbf84b6fb033460c92a17184
Publikováno v:
Cell Stress, Vol 8, Pp 69-82 (2024)
Exceeding physiological limits of the cell membrane potential compromises structural integrity, enabling the passage of normally impermeant solutes and disrupting cell function. Electropermeabilization has been studied extensively at the cellular sca
Externí odkaz:
https://doaj.org/article/55b6e8adaf35419c9ed03a5de82da14e
Publikováno v:
PLoS ONE, Vol 11, Iss 7, p e0159434 (2016)
Electroporation-induced cell sensitization was described as the occurrence of a delayed hypersensitivity to electric pulses caused by pretreating cells with electric pulses. It was achieved by increasing the duration of the electroporation treatment
Externí odkaz:
https://doaj.org/article/5952efe560d24f9da9089303cce0bb7c
Autor:
Fei Xie, Frency Varghese, Andrei G Pakhomov, Iurii Semenov, Shu Xiao, Jonathan Philpott, Christian Zemlin
Publikováno v:
PLoS ONE, Vol 10, Iss 12, p e0144833 (2015)
BACKGROUND:Ablation of cardiac tissue is an essential tool for the treatment of arrhythmias, particularly of atrial fibrillation, atrial flutter, and ventricular tachycardia. Current ablation technologies suffer from substantial recurrence rates, the
Externí odkaz:
https://doaj.org/article/a2ccb8c8842a4266ba7025ab4be991f1
Publikováno v:
PLoS ONE, Vol 8, Iss 7, p e70278 (2013)
High-amplitude electric pulses of nanosecond duration, also known as nanosecond pulsed electric field (nsPEF), are a novel modality with promising applications for cell stimulation and tissue ablation. However, key mechanisms responsible for the cyto
Externí odkaz:
https://doaj.org/article/70c3b3bfeb6b4b9c87be86ba1912073e
Autor:
Olga N Pakhomova, Betsy W Gregory, Vera A Khorokhorina, Angela M Bowman, Shu Xiao, Andrei G Pakhomov
Publikováno v:
PLoS ONE, Vol 6, Iss 2, p e17100 (2011)
Electroporation is a method of disrupting the integrity of cell membrane by electric pulses (EPs). Electrical modeling is widely employed to explain and study electroporation, but even most advanced models show limited predictive power. No studies ha
Externí odkaz:
https://doaj.org/article/1d631789cf5542899672b39370ee6443
Autor:
Bennett L Ibey, Caleb C Roth, Andrei G Pakhomov, Joshua A Bernhard, Gerald J Wilmink, Olga N Pakhomova
Publikováno v:
PLoS ONE, Vol 6, Iss 1, p e15642 (2011)
In this study, we determined the LD(50) (50% lethal dose) for cell death, and the ED(50) (50% of cell population staining positive) for propidium (Pr) iodide uptake, and phosphatidylserine (PS) externalization for several commonly studied cell lines
Externí odkaz:
https://doaj.org/article/2d3e38b681934ae4b56b0d1f3eda6e70
Autor:
Pamela W. Sowa, Aleksander S. Kiełbik, Andrei G. Pakhomov, Emily Gudvangen, Uma Mangalanathan, Volker Adams, Olga N. Pakhomova
Publikováno v:
Frontiers in Cardiovascular Medicine, Vol 9 (2022)
Electric shocks, the only effective therapy for ventricular fibrillation, also electroporate cardiac cells and contribute to the high-mortality post-cardiac arrest syndrome. Copolymers such as Poloxamer 188 (P188) are known to preserve the membrane i
Externí odkaz:
https://doaj.org/article/358634790a3b4390b8c7673860881b99
Autor:
Vitalii Kim, Iurii Semenov, Allen S. Kiester, Mark A. Keppler, Bennett L. Ibey, Joel N. Bixler, Ruben M. L. Colunga Biancatelli, Andrei G. Pakhomov
Publikováno v:
International Journal of Molecular Sciences, Vol 24, Iss 13, p 10921 (2023)
Reversing the pulse polarity, i.e., changing the electric field direction by 180°, inhibits electroporation and electrostimulation by nanosecond electric pulses (nsEPs). This feature, known as “bipolar cancellation,” enables selective remote tar
Externí odkaz:
https://doaj.org/article/9fdf99ff78644d5aa3bb432c3ae2016b
Autor:
Giedre Silkuniene, Uma M. Mangalanathan, Alessandra Rossi, Peter A. Mollica, Andrei G. Pakhomov, Olga Pakhomova
Publikováno v:
International Journal of Molecular Sciences, Vol 24, Iss 11, p 9191 (2023)
The study was aimed at identifying endogenous proteins which assist or impede the permeabilized state in the cell membrane disrupted by nsEP (20 or 40 pulses, 300 ns width, 7 kV/cm). We employed a LentiArray CRISPR library to generate knockouts (KOs)
Externí odkaz:
https://doaj.org/article/e8d6fc7665854ee3a58c33bde6a50352