Zobrazeno 1 - 10
of 105
pro vyhledávání: '"Andrei D. Mirzabekov"'
Publikováno v:
BioTechniques, Vol 29, Iss 4, Pp 844-857 (2000)
PCR amplification on a microarray of gel-immobilized primers (microchip) has been developed. One of a pair of PCR primers was immobilized inside a separate microchip polyacrylamide porous gel pad of 0.1 × 0.1 × 0.02 (or 0.04) μm in size and 0.2 (o
Externí odkaz:
https://doaj.org/article/f1a4efb1706f4c10a184a47d95e87d9e
Publikováno v:
Biosensors and Bioelectronics. 20:1860-1865
Advanced development of the hydrogel bacterial microchip (HBMChip) technique is proposed. The microchip represents an array of hemispherical gel elements 0.3-60 nl in volume attached to hydrophobic glass surface and containing live immobilized microb
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::171b4fccc14618c399cee28b441be552
https://doi.org/10.1016/j.bios.2004.06.005
https://doi.org/10.1016/j.bios.2004.06.005
Autor:
Andrei D. Mirzabekov, E L Darii, A. A. Stomakhin, V. E. Barsky, S M Ivanov, E V Konovalova, S V Pan'kov, A. Yu. Rubina, Ekaterina Dementieva
Publikováno v:
Scopus-Elsevier
Here a simple, reproducible, and versatile method is described for manufacturing protein and ligand chips. The photo-induced copolymerization of acrylamide-based gel monomers with different probes (oligonucleotides, DNA, proteins, and low-molecular l
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3d3af58dc71f17c4e85a6c38f95928f5
https://doi.org/10.2144/03345rr01
https://doi.org/10.2144/03345rr01
Publikováno v:
Molecular Biology. 37:617-630
Thermodynamic analysis was performed for the duplexes formed by fluorescently labeled oligonucleotide targets on a generic hexanucleotide microchip. All 4096 different hexanucleotide chains were immobilized as probes in individual gel pads of the mic
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::fb6ebb52e819a0ff40bac5c13a045f4b
https://doi.org/10.1023/a:1025103914659
https://doi.org/10.1023/a:1025103914659
Publikováno v:
Analytical Biochemistry. 311:103-118
DNA microchip technology is a rapid, high-throughput method for nucleic acid hybridization reactions. This technology requires random fragmentation and fluorescent labeling of target nucleic acids prior to hybridization. Radical-generating coordinati
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::39f1633b99495b1b774116fca752d128
https://doi.org/10.1016/s0003-2697(02)00388-3
https://doi.org/10.1016/s0003-2697(02)00388-3
Autor:
A Kolchinsky, M.A Skabkin, O. A. Zasedateleva, Andrei D. Mirzabekov, Dmitry Prokopenko, L.P Ovchinnikov, Alexander Krylov
Publikováno v:
Journal of Molecular Biology. 324:73-87
p50 protein is a member of the Y-box binding transcription factor family and is a counterpart of YB-1 protein. The generic microchip was used to analyze the sequence specificity of p50 binding to single (ss) and double-stranded (ds) oligodeoxyribonuc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::74abd6b272ee8362a52774d1a9033eac
https://doi.org/10.1016/s0022-2836(02)00937-3
https://doi.org/10.1016/s0022-2836(02)00937-3
Autor:
Andrei D. Mirzabekov, Alexander N. Perov, Ekaterina Kotova, Victor Barsky, Alexei Sharonov, Edward Kreindlin, Alexander V. Chudinov, Sergei Tokalov
Publikováno v:
SLAS Discovery. 7:247-257
A series of biochip readers developed for gel-based biochips includes three imaging models and a novel nonimaging biochip scanner. The imaging readers, ranging from a research-grade versatile reader to a simple portable one, use wide-field objectives
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::07b414218497276f79ad0837cef76868
https://doi.org/10.1177/108705710200700308
https://doi.org/10.1177/108705710200700308
Autor:
Maxim Mikheev, V. M. Mikhailovich, Blinov Vm, Sergei N. Shchelkunov, Elena Sokunova, Andrei D. Mirzabekov, Lev S. Sandakhchiev, Alexander Guskov, Alexander S. Zasedatelev, Sergey A. Lapa, I. V. Babkin, Alexander Y. Sobolev
Publikováno v:
Journal of Clinical Microbiology. 40:753-757
A method for species-specific detection of orthopoxviruses pathogenic for humans and animals is described. The method is based on hybridization of a fluorescently labeled amplified DNA specimen with the oligonucleotide DNA probes immobilized on a mic
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::41011cadc49eebb09dea07a2c5fa6894
https://doi.org/10.1128/jcm.40.3.753-757.2002
https://doi.org/10.1128/jcm.40.3.753-757.2002
Publikováno v:
Current Opinion in Chemical Biology. 6:70-75
Microarray format is the method of choice for high-throughput hybridization of nucleic acids. Lately, microarray strategy has been applied to broad studies of interactions of proteins with other molecules. The diversity of these interactions and vari
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3ef4294fc048843a7c34e8a847b21554
https://doi.org/10.1016/s1367-5931(01)00286-1
https://doi.org/10.1016/s1367-5931(01)00286-1
Autor:
Mikhail Perelman, Olga Irtuganova, D. A. Gryadunov, Alexander S. Zasedatelev, Larisa Chernousova, V. M. Mikhailovich, Sergey A. Lapa, Vladislav V. Erokhin, Boris N. Strizhkov, Andrei D. Mirzabekov, Alexander Y. Sobolev, Nikolai Chernyh, Arkadii Moroz, Vitalii Litvinov, Mikhail Vladimirskii, Olga Skotnikova
Publikováno v:
Journal of Clinical Microbiology. 39:2531-2540
Three new molecular approaches were developed to identify drug-resistant strains of Mycobacterium tuberculosis using biochips with oligonucleotides immobilized in polyacrylamide gel pads. These approaches are significantly faster than traditional bac
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::41941d95c41d590e608a3f4f69d9d194
https://doi.org/10.1128/jcm.39.7.2531-2540.2001
https://doi.org/10.1128/jcm.39.7.2531-2540.2001