Zobrazeno 1 - 10
of 97
pro vyhledávání: '"Andrei A Korostelev"'
Publikováno v:
Frontiers in Microbiology, Vol 15 (2024)
Ribosomes stall on truncated or otherwise damaged mRNAs. Bacteria rely on ribosome rescue mechanisms to replenish the pool of ribosomes available for translation. Trans-translation, the main ribosome-rescue pathway, uses a circular hybrid transfer-me
Externí odkaz:
https://doaj.org/article/0843585891bd4bfc81d5f89c22600307
Autor:
Anna B. Loveland, Egor Svidritskiy, Denis Susorov, Soojin Lee, Alexander Park, Sarah Zvornicanin, Gabriel Demo, Fen-Biao Gao, Andrei A. Korostelev
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-13 (2022)
The expansion of GGGGCC repeats in the C9ORF72 gene results in the production of disease causing abnormal proteins with polymeric glycine-arginine (poly-GR) and polymeric glycine-proline (poly-PR). Here the authors demonstrate a structural mechanism
Externí odkaz:
https://doaj.org/article/925d6997c2a04e6b9e28ab867faf90be
Autor:
Christine E. Carbone, Anna B. Loveland, Howard B. Gamper, Ya-Ming Hou, Gabriel Demo, Andrei A. Korostelev
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
EF-G drives ribosomal translocation along mRNA. Time-resolved cryo-EM captured translocation with EF-G•GTP—without inhibitors—revealing how EF-G uses ribosome fluctuations to drive translocation and GTP hydrolysis to leave at the right moment.
Externí odkaz:
https://doaj.org/article/4bea5e8abd3d4ecd81046f7ade0b17b2
Autor:
Gabriel Demo, Howard B. Gamper, Anna B. Loveland, Isao Masuda, Christine E. Carbone, Egor Svidritskiy, Ya-Ming Hou, Andrei A. Korostelev
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
Translational frameshifting is a mechanism that expands the coding capabilities of mRNA. Here, structures of 70S ribosome complexes with GTPase elongation factor G (EF-G), a +1-frameshifting-prone mRNA and tRNAs reveal the cooperation between the rib
Externí odkaz:
https://doaj.org/article/9e96fbbefbf946cca2e64e5aa10017b3
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-9 (2020)
Alternative rescue factor B (ArfB) is an enzyme that releases peptides from stalled ribosomes to allow ribosome recycling. Here the authors carry-out cryo-EM analyses of 70S ribosomes complexed with ArfB on either a short or longer mRNA to reveal dis
Externí odkaz:
https://doaj.org/article/7ffabda129144820b3a56d356ee51d8b
Autor:
Shijie Huang, Nikolay A. Aleksashin, Anna B. Loveland, Dorota Klepacki, Kaspar Reier, Amira Kefi, Teresa Szal, Jaanus Remme, Luc Jaeger, Nora Vázquez-Laslop, Andrei A. Korostelev, Alexander S. Mankin
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-13 (2020)
Ribosomes of all organisms have retained 5S rRNA as an autonomous rRNA species. Here the authors engineer a bacterial strain with ribosomes that do not have free 5S rRNA, and carry structural analyses that suggest the evolutionary preservation of 5S
Externí odkaz:
https://doaj.org/article/97ce74dddff14abd9b4d20a9c96f526d
Autor:
Hung-Lun Hsu, Alexander Brown, Anna B. Loveland, Anoushka Lotun, Meiyu Xu, Li Luo, Guangchao Xu, Jia Li, Lingzhi Ren, Qin Su, Dominic J. Gessler, Yuquan Wei, Phillip W. L. Tai, Andrei A. Korostelev, Guangping Gao
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-14 (2020)
Adeno-associated viruses (AAVs) are vehicles for gene therapy in humans, but currently only a limited amount of AAV serotypes is available. Here, the authors identify a novel AAV, AAVv66, and demonstrate enhanced production yields, virion stability,
Externí odkaz:
https://doaj.org/article/143cfb7a1596401ea3955f040a832c0f
Autor:
Chen Bao, Sarah Loerch, Clarence Ling, Andrei A Korostelev, Nikolaus Grigorieff, Dmitri N Ermolenko
Publikováno v:
eLife, Vol 9 (2020)
Although the elongating ribosome is an efficient helicase, certain mRNA stem-loop structures are known to impede ribosome movement along mRNA and stimulate programmed ribosome frameshifting via mechanisms that are not well understood. Using biochemic
Externí odkaz:
https://doaj.org/article/716549eb2dca4eb8a1914d4e9a7819df
Publikováno v:
Scientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
Abstract Eubacterial ribosomal large-subunit methyltransferase H (RlmH) methylates 23S ribosomal RNA pseudouridine 1915 (Ψ1915), which lies near the ribosomal decoding center. The smallest member of the SPOUT superfamily of methyltransferases, RlmH
Externí odkaz:
https://doaj.org/article/d054ded6d21e412c86951938f4ea5fff
Publikováno v:
eLife, Vol 8 (2019)
Protein synthesis ends when a ribosome reaches an mRNA stop codon. Release factors (RFs) decode the stop codon, hydrolyze peptidyl-tRNA to release the nascent protein, and then dissociate to allow ribosome recycling. To visualize termination by RF2,
Externí odkaz:
https://doaj.org/article/0da449cd4d5640eea3f29f0c324f4e4b