An objective, automated and robust scoring using fluorescence optical imaging to evaluate changes in micro-vascularisation indicating early arthritis

Autor: Lukas Zerweck, Michaela Köhm, Phuong-Ha Nguyen, Gerd Geißlinger, Frank Behrens, Andreas Pippow

Publikováno v: PLoS ONE, Vol 17, Iss 9 (2022)

Fluorescence optical imaging technique (FOI) is a well-established and valid method for visualization of changes in micro vascularization at different organ systems. As increased vascularization is an early feature of joint inflammation, FOI is a pro

Externí odkaz: https://doaj.org/article/c0252081b4704cee8e175a72005b8881

Analysis of neuronal Ca

Autor: Simon, Hess, Christophe, Pouzat, Lars, Paeger, Andreas, Pippow, Peter, Kloppenburg

Publikováno v: Cell calcium. 97

Ca

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::c1287c238e730baab3338960c4bcd527
https://pubmed.ncbi.nlm.nih.gov/34082340

Analysis of neuronal Ca2+ handling properties by combining perforated patch clamp recordings and the added buffer approach

Autor: Peter Kloppenburg, Lars Paeger, Andreas Pippow, Simon Hess, Christophe Pouzat

Publikováno v: Cell Calcium
Cell Calcium, Elsevier, In press, ⟨10.1016/j.ceca.2021.102411⟩
Cell Calcium, Elsevier, 2021, 97, pp.102411. ⟨10.1016/j.ceca.2021.102411⟩

International audience; Ca 2+ functions as an important intracellular signal for a wide range of cellular processes. These processes are selectively activated by controlled spatiotemporal dynamics of the free cytosolic Ca 2+ . Intracellular Ca 2+ dyn

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cfa73291612ea4bc2004b328ad6b46d6
https://doi.org/10.1016/j.ceca.2021.102411

Estimating background-subtracted fluorescence transients in calcium imaging experiments: A quantitative approach

Autor: Sébastien Joucla, Andreas Pippow, Romain Franconville, Peter Kloppenburg, Christophe Pouzat

Publikováno v: Cell Calcium. 54:71-85

Calcium imaging has become a routine technique in neuroscience for subcellular to network level investigations. The fast progresses in the development of new indicators and imaging techniques call for dedicated reliable analysis methods. In particula

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7288d1a0f14eb4fe35e9c78df5a60031
https://doi.org/10.1016/j.ceca.2013.04.005

Quantitative estimation of calcium dynamics from ratiometric measurements: a direct, nonratioing method

Autor: Andreas Pippow, Christophe Pouzat, Peter Kloppenburg, Sébastien Joucla

Publikováno v: Journal of Neurophysiology
Journal of Neurophysiology, American Physiological Society, 2010, 103 (2), pp.1130-44. 〈10.1152/jn.00414.2009〉
Journal of Neurophysiology, American Physiological Society, 2010, 103 (2), pp.1130-44. ⟨10.1152/jn.00414.2009⟩

International audience; Measuring variations of intracellular free calcium concentration through the changes in fluorescence of a calcium-sensitive dye is a ubiquitous technique in neuroscience. Despite its popularity, confidence intervals (CIs) on t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d55a2b7531d8d49a139b23d88a894160
https://pubmed.ncbi.nlm.nih.gov/19955286