Sensitivity enhancement of homonuclear multidimensional NMR correlations for labile sites in proteins, polysaccharides, and nucleic acids

Autor: Mihajlo Novakovic, Ēriks Kupče, Andreas Oxenfarth, Marcos D. Battistel, Darón I. Freedberg, Harald Schwalbe, Lucio Frydman

Publikováno v: Nature Communications, Vol 11, Iss 1, Pp 1-12 (2020)

Here, the authors present an approach that enhances the sensitivity of basic 2D biomolecular NMR experiments like NOESY and TOCSY, when carried out in polysaccharides, proteins and nucleic acids. This method combines principles associated to quantum

Externí odkaz: https://doaj.org/article/706ccc8d99ec4f9aa4ca1298fa4b223f

1H, 13C and 15N chemical shift assignment of the stem-loops 5b + c from the 5′-UTR of SARS-CoV-2

Autor: Klara R. Mertinkus, J. Tassilo Grün, Nadide Altincekic, Jasleen Kaur Bains, Betül Ceylan, Jan-Peter Ferner, Lucio Frydman, Boris Fürtig, Martin Hengesbach, Katharina F. Hohmann, Daniel Hymon, Jihyun Kim, Božana Knezic, Mihajlo Novakovic, Andreas Oxenfarth, Stephen A. Peter, Nusrat S. Qureshi, Christian Richter, Tali Scherf, Andreas Schlundt, Robbin Schnieders, Harald Schwalbe, Elke Stirnal, Alexey Sudakov, Jennifer Vögele, Anna Wacker, Julia E. Weigand, Julia Wirmer-Bartoschek, Maria A. Wirtz Martin, Jens Wöhnert

Publikováno v: Biomolecular NMR Assignments, 16

The ongoing pandemic of the respiratory disease COVID-19 is caused by the SARS-CoV-2 (SCoV2) virus. SCoV2 is a member of the Betacoronavirus genus. The 30 kb positive sense, single stranded RNA genome of SCoV2 features 5'- and 3'-genomic ends that ar

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::99f387aa7cf6d5f0b5a6907a6bb95fa8
https://hdl.handle.net/20.500.11850/534661

The Extended Hadamard Transform: Sensitivity-Enhanced NMR Experiments Among Labile and Non-Labile

Autor: Jihyun, Kim, Mihajlo, Novakovic, Sundaresan, Jayanthi, Adonis, Lupulescu, Ēriks, Kupče, J Tassilo, Grün, Klara, Mertinkus, Andreas, Oxenfarth, Harald, Schwalbe, Lucio, Frydman

Publikováno v: Chemphyschem : a European journal of chemical physics and physical chemistry. 23(4)

Hadamard encoded saturation transfer can significantly improve the efficiency of NOE-based NMR correlations from labile protons in proteins, glycans and RNAs, increasing the sensitivity of cross-peaks by an order of magnitude and shortening experimen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::1b6b8b6e866399a425370e289238c954
https://pubmed.ncbi.nlm.nih.gov/34968005

Magnetization Transfer to Enhance NOE Cross‐Peaks among Labile Protons: Applications to Imino–Imino Sequential Walks in SARS‐CoV‐2‐Derived RNAs

Autor: Andreas Oxenfarth, Mihajlo Novakovic, Robbin Schnieders, Eriks Kupče, Tali Scherf, Harald Schwalbe, J Tassilo Grün, Julia Wirmer-Bartoschek, Christian Richter, Lucio Frydman

Publikováno v: Angewandte Chemie International Edition
Angewandte Chemie (International Ed. in English)
Angewandte Chemie (Weinheim an Der Bergstrasse, Germany)

2D NOESY plays a central role in structural NMR spectroscopy. We have recently discussed methods that rely on solvent‐driven exchanges to enhance NOE correlations between exchangeable and non‐exchangeable protons in nucleic acids. Such methods, h

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::773193e7ee738be4afbc17be4e81cfbf

19F‐NMR‐based fragment screening for 14 different biologically active RNAs and 10 DNA and protein counter‐screens

Autor: Tatjana Schamber, Kamal Azzaoui, Nusrat S. Qureshi, Boris Fürtig, Oliver Binas, Jason N Martins, Marcel J. J. Blommers, Daniel Hymon, Alix Tröster, Jasleen Kaur Bains, Christian Richter, Harald Schwalbe, Elke Stirnal, Andreas Oxenfarth, Julia Wirmer-Bartoschek, Hannes Berg, Anna Wacker, Robbin Schnieders, Tom Landgraf, Vanessa de Jesus, Santosh Lakshmi Gande, Sridhar Sreeramulu, Thomas Biedenbänder, Maria Alexandra Wirtz Martin, Albrecht Eduard Völklein, Anna Niesteruk

Publikováno v: ChemBioChem
Chembiochem

We report here on the nuclear magnetic resonance (NMR) 19 F screening of 14 RNA targets with different secondary and tertiary structure to systematically assess druggability of RNAs. Our RNA targets include representative bacterial riboswitches that

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::57aa4c3116edf518bcf60528cde01fe5