In Vivo Knockout of the Vegfa Gene by Lentiviral Delivery of CRISPR/Cas9 in Mouse Retinal Pigment Epithelium Cells

Autor: Andreas Holmgaard, Anne Louise Askou, Josephine Natalia Esther Benckendorff, Emil Aagaard Thomsen, Yujia Cai, Toke Bek, Jacob Giehm Mikkelsen, Thomas J. Corydon

Publikováno v: Molecular Therapy: Nucleic Acids, Vol 9, Iss C, Pp 89-99 (2017)

Virus-based gene therapy by CRISPR/Cas9-mediated genome editing and knockout may provide a new option for treatment of inherited and acquired ocular diseases of the retina. In support of this notion, we show that Streptococcus pyogenes (Sp) Cas9, del

Externí odkaz: https://doaj.org/article/161da74f106c4bfcac63b2f9d8bea9df

CRISPR Gene Therapy of the Eye: Targeted Knockout of Vegfa in Mouse Retina by Lentiviral Delivery

Autor: Andreas, Holmgaard, Sidsel, Alsing, Anne Louise, Askou, Thomas J, Corydon

Publikováno v: Methods in molecular biology (Clifton, N.J.). 1961

Genome editing and knockout by virus-based delivery of CRISPR/Cas9 may provide a new option to cure inherited and acquired ocular diseases. Here we describe development and application of lentivirus-based delivery vectors enabling knockout of the Veg

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::62de043e321d446b362249ff2893567e
https://pubmed.ncbi.nlm.nih.gov/30912054

Suppression of Choroidal Neovascularization by AAV-Based Dual-Acting Antiangiogenic Gene Therapy

Autor: Josephine Natalia Esther Benckendorff, Jacob Giehm Mikkelsen, Anne Louise Askou, Sidsel Alsing, Lars Aagaard, Toke Bek, Andreas Holmgaard, Thomas J. Corydon

Publikováno v: Molecular Therapy. Nucleic Acids
Askou, A L, Alsing, S, Benckendorff, J N E, Holmgaard, A, Mikkelsen, J G, Aagaard, L, Bek, T & Corydon, T J 2019, ' Suppression of Choroidal Neovascularization by AAV-Based Dual-Acting Antiangiogenic Gene Therapy ', Molecular Therapy-Nucleic Acids, vol. 16, pp. 38-50 . https://doi.org/10.1016/j.omtn.2019.01.012
Molecular Therapy: Nucleic Acids, Vol 16, Iss, Pp 38-50 (2019)

Vascular endothelial growth factor A (VEGFA) is involved in the pathogenesis of vasoproliferative retinal diseases, such as exudative age-related macular degeneration (AMD). The objective of this study was to investigate whether dual-acting therapy b

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9d79973599daaa521b3b2c54e8891c36
https://pubmed.ncbi.nlm.nih.gov/30825671

Suppression of Choroidal Neovascularization in Mice by Subretinal Delivery of Multigenic Lentiviral Vectors Encoding Anti-Angiogenic MicroRNAs

Autor: Lars Aagaard, Thomas J. Corydon, Toke Bek, Anne Louise Askou, Tina Storm, Josephine Natalia Esther Benckendorff, Andreas Holmgaard, Jacob Giehm Mikkelsen

Publikováno v: Askou, A L, Benckendorff, J N E, Holmgaard, A, Storm, T, Aagaard, L, Bek, T, Mikkelsen, J G & Corydon, T J 2017, ' Suppression of Choroidal Neovascularization in Mice by Subretinal Delivery of Multigenic Lentiviral Vectors Encoding Anti-Angiogenic MicroRNAs ', Human Gene Therapy. Methods, vol. 28, no. 4, pp. 222-233 . https://doi.org/10.1089/hgtb.2017.079

Lentivirus-based vectors have been used for the development of potent gene therapies. Here, application of a multigenic lentiviral vector (LV) producing multiple anti-angiogenic microRNAs following subretinal delivery in a laser-induced choroidal neo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cfb75153ee6e63a1afdcec0ba1c21696
https://pure.au.dk/portal/da/publications/suppression-of-choroidal-neovascularization-in-mice-by-subretinal-delivery-of-multigenic-lentiviral-vectors-encoding-antiangiogenic-micrornas(ba7f28e8-5def-4baf-869c-f33e0a4e51b3).html