CRISPR-Cas9 Editing of Human Histone Deubiquitinase Gene USP16 in Human Monocytic Leukemia Cell Line THP-1

Autor: Iveta Gažová, Lucas Lefevre, Stephen J. Bush, Rocio Rojo, David A. Hume, Andreas Lengeling, Kim M. Summers

Publikováno v: Frontiers in Cell and Developmental Biology, Vol 9 (2021)

USP16 is a histone deubiquitinase which facilitates G2/M transition during the cell cycle, regulates DNA damage repair and contributes to inducible gene expression. We mutated the USP16 gene in a high differentiation clone of the acute monocytic leuk

Externí odkaz: https://doaj.org/article/007df63a40774d17a153ba289c1c7a34

JMJD6 cleaves MePCE to release positive transcription elongation factor b (P-TEFb) in higher eukaryotes

Autor: Schuyler Lee, Haolin Liu, Ryan Hill, Chunjing Chen, Xia Hong, Fran Crawford, Molly Kingsley, Qianqian Zhang, Xinjian Liu, Zhongzhou Chen, Andreas Lengeling, Kathrin Maria Bernt, Philippa Marrack, John Kappler, Qiang Zhou, Chuan-Yuan Li, Yuhua Xue, Kirk Hansen, Gongyi Zhang

Publikováno v: eLife, Vol 9 (2020)

More than 30% of genes in higher eukaryotes are regulated by promoter-proximal pausing of RNA polymerase II (Pol II). Phosphorylation of Pol II CTD by positive transcription elongation factor b (P-TEFb) is a necessary precursor event that enables pro

Externí odkaz: https://doaj.org/article/f198e846e7ef4c6dbecd3c894c262736

The Staphylococcus aureus superantigen SElX is a bifunctional toxin that inhibits neutrophil function.

Autor: Stephen W Tuffs, David B A James, Jovanka Bestebroer, Amy C Richards, Mariya I Goncheva, Marie O'Shea, Bryan A Wee, Keun Seok Seo, Patrick M Schlievert, Andreas Lengeling, Jos A van Strijp, Victor J Torres, J Ross Fitzgerald

Publikováno v: PLoS Pathogens, Vol 13, Iss 9, p e1006461 (2017)

Bacterial superantigens (SAgs) cause Vβ-dependent T-cell proliferation leading to immune dysregulation associated with the pathogenesis of life-threatening infections such as toxic shock syndrome, and necrotizing pneumonia. Previously, we demonstrat

Externí odkaz: https://doaj.org/article/ae54e40195464ed8bfce66dbe8119856

JMJD6 promotes self-renewal and regenerative capacity of hematopoietic stem cells

Autor: Jozef Durko, Andrea Tavosanis, Peter Giles, Hannah Lawson, Alena Shmakova, Nicholas M. Morton, Douglas Vernimmen, Dónal O'Carroll, Marie O'Shea, Neil P. Rodrigues, Amelie V. Guitart, Melania Barile, Andreas Lengeling, Kamil R. Kranc, Roderick N. Carter, Berthold Göttgens, Elise Georges, Milica Vukovic, Lewis Allen, Penny Timms, Catarina Sepulveda, Christopher J. Schofield, Joana Campos, Louie N. van de Lagemaat

Publikováno v: Blood Adv
Lawson, H, Sepulveda, C, van de Lagemaat, L N, Durko, J, Barile, M, Tavosanis, A, Georges, E, Shmakova, A, Timms, P, Carter, R N, Allen, L, Campos, J, Vukovic, M, Guitart, A V, Giles, P, O'Shea, M, Vernimmen, D, Morton, N M, Rodrigues, N P, Göttgens, B, Schofield, C J, Lengeling, A, O'Carroll, D & Kranc, K R 2021, ' JMJD6 promotes self-renewal and regenerative capacity of hematopoietic stem cells ', Blood Advances, vol. 5, no. 3, pp. 889-899 . https://doi.org/10.1182/bloodadvances.2020002702

Lifelong multilineage hematopoiesis critically depends on rare hematopoietic stem cells (HSCs) that reside in the hypoxic bone marrow microenvironment. Although the role of the canonical oxygen sensor hypoxia-inducible factor prolyl hydroxylase has b

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e9ce5b949122a2f262c58d0ad509090

Phylogenetic and genomic analyses of the ribosomal oxygenases Riox1 (No66) and Riox2 (Mina53) provide new insights into their evolution

Autor: Jasmin Moneer, Annette Feuchtinger, Katharina E Bräuer, Kevin Brockers, Andreas Lengeling, Evi Wollscheid-Lengeling, Alexander Wolf

Publikováno v: BMC Evolutionary Biology, Vol 18, Iss 1, Pp 1-16 (2018)
BMC Evolutionary Biology
Bräuer, K E, Brockers, K, Moneer, J, Feuchtinger, A, Wollscheid-Lengeling, E, Lengeling, A & Wolf, A 2018, ' Phylogenetic and genomic analyses of the ribosomal oxygenases Riox1 (No66) and Riox2 (Mina53) provide new insights into their evolution ', BMC Evolutionary Biology, vol. 18, no. 1, pp. 96 . https://doi.org/10.1186/s12862-018-1215-0
BMC Evol. Biol. 18:96 (2018)

Background Translation of specific mRNAs can be highly regulated in different cells, tissues or under pathological conditions. Ribosome heterogeneity can originate from variable expression or post-translational modifications of ribosomal proteins. Th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ca6825dec8495a5c5b3ce476a2793cf3
http://link.springer.com/article/10.1186/s12862-018-1215-0