Kinetics of cyclobutane thymine dimer splitting by DNA photolyase directly monitored in the UV

Autor: Martin Byrdin, Klaus Brettel, Pavel Müller, Viruthachalam Thiagarajan, André P. M. Eker

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America
Proceedings of the National Academy of Sciences of the United States of America, 2011, 108 (23), pp.9402-9407. ⟨10.1073/pnas.1101026108⟩
Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2011, 108 (23), pp.9402-9407. ⟨10.1073/pnas.1101026108⟩
Proceedings of the National Academy of Sciences of the U.S.A., 108(23), 9402-9407. National Academy of Sciences

CPD photolyase uses light to repair cyclobutane pyrimidine dimers (CPDs) formed between adjacent pyrimidines in UV-irradiated DNA. The enzyme harbors an FAD cofactor in fully reduced state (FADH - ). The CPD repair mechanism involves electron transfe

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::44f6a44795d5d26bfcd502562622e4b6
https://doi.org/10.1073/pnas.1101026108

DNA Repair by Photolyase: A Novel Substrate with Low Background Absorption around 265 nm for Transient Absorption Studies in the UV

Autor: André P. M. Eker, Klaus Brettel, Viruthachalam Thiagarajan, Martin Byrdin, Sandrine Villette, Agathe Espagne

Publikováno v: Biochemistry, 49(2), 297-303. American Chemical Society
Biochemistry
Biochemistry, 2010, 49 (2), pp.297-303. ⟨10.1021/bi901562a⟩
Biochemistry, American Chemical Society, 2010, 49 (2), pp.297-303. ⟨10.1021/bi901562a⟩

International audience; CPD photolyase enzymatically repairs the major UV-induced lesion in DNA, the cyclobutane pyrimidine dimer (CPD), by photoreversion of the damage reaction. An enzyme-bound reduced flavin (FADH(-)) cofactor functions its photose

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::95347b25bdcf96f7fa7c07cfe4c96efd
https://doi.org/10.1021/bi901562a

Polarized transient absorption to resolve electron transfer between tryptophans in DNA photolyase

Autor: André P. M. Eker, Martin Byrdin, Sandrine Villette, Agathe Espagne, Klaus Brettel

Publikováno v: Journal of Physical Chemistry B
Journal of Physical Chemistry B, American Chemical Society, 2008, 112, pp.6866-6871. ⟨10.1021/jp711435y⟩
Journal of Physical Chemistry B, 112(22), 6866-6871. American Chemical Society
Journal of Physical Chemistry B, 2008, 112, pp.6866-6871. ⟨10.1021/jp711435y⟩

International audience; Transient absorption spectroscopy is a powerful tool for studying biological electron-transfer chains, provided that their members give rise to distinct changes of their absorption spectra. There are, however, chains that cont

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cfa7ee1d4f39c2affcae0e6096db2e1f
https://hdl.handle.net/1765/56401

Crystal structure of a photolyase bound to a CPD-like lesion after in situ repair

Autor: Lars-Oliver Essen, Ulrich Hennecke, Petra Gnau, Tobias Klar, André P. M. Eker, Alexandra Mees, Thomas Carell

Publikováno v: Science, 306, 1789-1793. American Association for the Advancement of Science

DNA photolyases use light energy to repair DNA that comprises ultraviolet-induced lesions such as the cis-syn cyclobutane pyrimidine dimers (CPDs). Here we report the crystal structure of a DNA photolyase bound to duplex DNA that is bent by 50° and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::94ee2980df9f30c151b39be634a81c46
https://pure.eur.nl/en/publications/08f24a83-3307-4425-bebc-d3a8cab3591f

Intraprotein radical transfer during photoactivation of DNA photolyase

Autor: Corinne Aubert, Marten H. Vos, Paul Mathis, Klaus Brettel, André P. M. Eker

Publikováno v: Nature, 405, 586-590. Nature Publishing Group
Nature
Nature, Nature Publishing Group, 2000, 405 (1 june), pp.586. ⟨10.1038/35014644⟩

International audience; Amino-acid radicals play key roles in many enzymatic reactions. Catalysis often involves transfer of a radical character within the protein, as in class I ribonucleotide reductase where radical transfer occurs over 35 Å, from

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::46d566025c0c0b98170ba927bb135e03
https://pure.eur.nl/en/publications/b8651f50-cb34-427d-95d6-cd86061154c9