Zobrazeno 1 - 10
of 13
pro vyhledávání: '"André Gemeinhardt"'
Publikováno v:
Optical Sensors and Sensing Congress 2022 (AIS, LACSEA, Sensors, ES).
Resolving polydispersions in the nanometer regime remains a challenge for state-of-the-art particle sizers. By combining nanoparticle tracking analysis with interferometric scattering microscopy, we precisely estimated the size and refractive index o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::fa77865aeffd3c683e5a3787dfaa29dd
https://doi.org/10.1364/sensors.2022.sw3e.1
https://doi.org/10.1364/sensors.2022.sw3e.1
Characterization of the size and material properties of particles in liquid suspensions is in very high demand, e.g., for the analysis of colloidal samples or of bodily fluids such as urine or blood plasma. However, the existing methods are limited i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8944f11c0d2c9039791f6ebedb81a656
https://doi.org/10.1101/2021.11.13.468485
https://doi.org/10.1101/2021.11.13.468485
Autor:
Houman Mirzaalian Dastjerdi, Mahyar Dahmardeh, André Gemeinhardt, Vahid Sandoghdar, Reza Gholami Mahmoodabadi, Harald Köstler
It has been shown that interferometric detection of Rayleigh scattering (iSCAT) can reach an exquisite sensitivity for label-free detection of nano-matter, down to single proteins. The sensitivity of iSCAT detection is intrinsically limited by shot n
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fd3f06e474f1e73215a40ca4b2a3863a
https://opus4.kobv.de/opus4-fau/files/18238/d_55_5_054002.pdf
https://opus4.kobv.de/opus4-fau/files/18238/d_55_5_054002.pdf
Publikováno v:
Nature methods. 19(5)
Characterization of the size and material properties of particles in liquid suspensions is in very high demand, for example, in the analysis of colloidal samples or of bodily fluids such as urine or blood plasma. However, existing methods are limited
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::51f241801a73ef7cf35ede52df5e2747
https://pubmed.ncbi.nlm.nih.gov/35534632
https://pubmed.ncbi.nlm.nih.gov/35534632
Publikováno v:
BIOspektrum. 25:732-736
iSCAT microscopy is a powerful tool for the optical detection and visualization of small nanoparticles, down to single unlabeled proteins. In this overview, we give an introduction to the method’s principles and benefits and show how it can be appl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::fd5f71aed6de5a8fe3072ba9a4a3c979
https://doi.org/10.1007/s12268-019-0225-9
https://doi.org/10.1007/s12268-019-0225-9
Publikováno v:
Biophotonics Congress: Biomedical Optics 2020 (Translational, Microscopy, OCT, OTS, BRAIN).
We use interferometric scattering microscopy (iSCAT) to study in-situ secretion dynamics of living cells. The method allows us to optically detect single proteins in real-time without the need for labeling.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::52bf65c0cf6bd3efb074524141bff347
https://doi.org/10.1364/microscopy.2020.mtu1a.3
https://doi.org/10.1364/microscopy.2020.mtu1a.3
Autor:
Vahid Sandoghdar, Michael Aigner, Katharina König, Matthew P. McDonald, André Gemeinhardt, Simon Völkl, Marek Piliarik, Stefanie Schaffer, Andreas Mackensen
Publikováno v:
Nano Letters. 18:513-519
Cellular secretion of proteins into the extracellular environment is an essential mediator of critical biological mechanisms, including cell-to-cell communication, immunological response, targeted delivery, and differentiation. Here, we report a nove
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b3abb6a6eacaf547e4b07c0cc3f6998b
https://doi.org/10.1021/acs.nanolett.7b04494
https://doi.org/10.1021/acs.nanolett.7b04494
Autor:
André Gemeinhardt, Reza Gholami Mahmoodabadi, Katharina König, Mahyar Dahmardeh, Houman Mirzaalian Dastjerdi, Vahid Sandoghdar, Francesca Nicoli, Harald Köstler
Publikováno v:
2019 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC).
In recent decades large efforts have been made to push optical imaging to the limit, for example for detecting very small proteins. Great achievements have been obtained with fluorescence microscopy, both in terms of localization precision and study
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::aaececf2f27c20ece7d0563aab692d6d
https://doi.org/10.1109/cleoe-eqec.2019.8872414
https://doi.org/10.1109/cleoe-eqec.2019.8872414
Publikováno v:
2019 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC).
Proteins are involved in a large number of biological processes. Apart from the field of proteomics, where ensemble studies of the entire set of proteins present in a system are carried out, detection and analysis of single proteins has become a vibr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::550dae1ca7274cafe94b49892e21bf8b
https://doi.org/10.1109/cleoe-eqec.2019.8872994
https://doi.org/10.1109/cleoe-eqec.2019.8872994
Autor:
A. D. Kashkanova, A. B. Shkarin, Yazgan Tuna, Vahid Sandoghdar, André Gemeinhardt, Martin Blessing, Reza Gholami Mahmoodabadi
Publikováno v:
Optics Express. 29:11070
We introduce an image transform designed to highlight features with high degree of radial symmetry for identification and subpixel localization of particles in microscopy images. The transform is based on analyzing pixel value variations in radial an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::62db7fb251eb79a311af45e69f8f7896
https://doi.org/10.1364/oe.420670
https://doi.org/10.1364/oe.420670