Development of an in vitro method for activation of X-succinate synthases for fumarate hydroalkylation.

Autor: Andorfer MC; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA., King-Roberts DT; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Imrich CN; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Brotheridge BG; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Drennan CL; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.; Center for Environmental Health, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.; Bio-inspired Solar Energy Program, Canadian Institute for Advanced Research (CIFAR), Toronto, ON M5G 1M1, Canada.

Publikováno v: IScience [iScience] 2023 May 19; Vol. 26 (6), pp. 106902. Date of Electronic Publication: 2023 May 19 (Print Publication: 2023).

A haem-sequestering plant peptide promotes iron uptake in symbiotic bacteria.

Autor: Sankari S; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA., Babu VMP; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA., Bian K; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA., Alhhazmi A; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.; Medical Laboratory Technology, College of Applied Medical Sciences, Taibah University, Madinah, Saudi Arabia., Andorfer MC; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA, USA., Avalos DM; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA, USA.; Harvard Graduate Program in Biophysics, Harvard University, Cambridge, MA, USA., Smith TA; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.; Whitehead Institute for Biomedical Research, Cambridge, MA, USA., Yoon K; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA., Drennan CL; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA, USA.; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, USA., Yaffe MB; Departments of Biology and Biological Engineering, and Center for Precision Cancer Medicine, David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute for Technology, Cambridge, MA, USA.; Divisions of Acute Care Surgery, Trauma, and Surgical Critical Care, and Surgical Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA., Lourido S; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.; Whitehead Institute for Biomedical Research, Cambridge, MA, USA., Walker GC; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA. gwalker@mit.edu.

Publikováno v: Nature microbiology [Nat Microbiol] 2022 Sep; Vol. 7 (9), pp. 1453-1465. Date of Electronic Publication: 2022 Aug 11.

Molecular basis for catabolism of the abundant metabolite trans -4-hydroxy-L-proline by a microbial glycyl radical enzyme.

Autor: Backman LR; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, United States., Huang YY; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, United States., Andorfer MC; Department of Biology, Massachusetts Institute of Technology, Cambridge, United States., Gold B; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, United States., Raines RT; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, United States., Balskus EP; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, United States., Drennan CL; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, United States.; Department of Biology, Massachusetts Institute of Technology, Cambridge, United States.; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, United States.

Publikováno v: ELife [Elife] 2020 Mar 17; Vol. 9. Date of Electronic Publication: 2020 Mar 17.

Solution structure and biochemical characterization of a spare part protein that restores activity to an oxygen-damaged glycyl radical enzyme.

Autor: Bowman SEJ; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Building 68-680, Cambridge, MA, 02139, USA.; Department of Chemistry, Massachusetts Institute of Technology, Building 68-680, Cambridge, MA, 02139, USA.; Hauptman-Woodward Medical Research Institute, Buffalo, NY, 14203, USA., Backman LRF; Department of Chemistry, Massachusetts Institute of Technology, Building 68-680, Cambridge, MA, 02139, USA.; MIT Summer Research Program (MSRP), Massachusetts Institute of Technology, Cambridge, MA, 02139, USA., Bjork RE; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Building 68-680, Cambridge, MA, 02139, USA., Andorfer MC; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Building 68-680, Cambridge, MA, 02139, USA.; Department of Biology, Massachusetts Institute of Technology, Building 68-680, Cambridge, MA, 02139, USA., Yori S; MIT Summer Research Program (MSRP), Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.; Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, CA, 94720, USA., Caruso A; MIT Summer Research Program (MSRP), Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.; Department of Chemistry, Princeton University, Princeton, NJ, 08544, USA., Stultz CM; Electrical Engineering and Computer Science and Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA, 02319, USA.; Division of Cardiovascular Medicine, Massachusetts General Hospital, Boston, MA, 02114, USA., Drennan CL; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Building 68-680, Cambridge, MA, 02139, USA. cdrennan@mit.edu.; Department of Chemistry, Massachusetts Institute of Technology, Building 68-680, Cambridge, MA, 02139, USA. cdrennan@mit.edu.; Department of Biology, Massachusetts Institute of Technology, Building 68-680, Cambridge, MA, 02139, USA. cdrennan@mit.edu.; Center for Environmental Health, Massachusetts Institute of Technology, Building 68-680, Cambridge, MA, 02139, USA. cdrennan@mit.edu.

Publikováno v: Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry [J Biol Inorg Chem] 2019 Sep; Vol. 24 (6), pp. 817-829. Date of Electronic Publication: 2019 Jun 27.

Aromatic Halogenation by Using Bifunctional Flavin Reductase-Halogenase Fusion Enzymes.

Autor: Andorfer MC; Department of Chemistry, University of Chicago, 5735 South Ellis Avenue, SCL 302, Chicago, IL, 60637, USA., Belsare KD; Department of Chemistry, University of Chicago, 5735 South Ellis Avenue, SCL 302, Chicago, IL, 60637, USA., Girlich AM; Department of Chemistry, University of Chicago, 5735 South Ellis Avenue, SCL 302, Chicago, IL, 60637, USA., Lewis JC; Department of Chemistry, University of Chicago, 5735 South Ellis Avenue, SCL 302, Chicago, IL, 60637, USA.

Publikováno v: Chembiochem : a European journal of chemical biology [Chembiochem] 2017 Nov 02; Vol. 18 (21), pp. 2099-2103. Date of Electronic Publication: 2017 Sep 22.