Isotopic labeling of recombinant proteins expressed in the protozoan host Leishmania tarentolae

Autor: Mathias Gruen, Ion Cristian Cirstea, Reinhard Breitling, Tim Bergbrede, Kirill Alexandrov, Regina Pietrucha, Peter Bayer, Anca Niculae

Publikováno v: Protein Expression and Purification. 48:167-172

Isotope labeling of recombinant proteins is a prerequisite for application of nuclear magnetic resonance spectroscopy (NMR) for the characterization of the three-dimensional structures and dynamics of proteins. Overexpression of isotopically labeled

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b7a59fb260a1300e3d494f55fed1c929
https://doi.org/10.1016/j.pep.2006.04.006

Expression of mammalian Rab Escort Protein-1 and -2 in yeast Saccharomyces cerevisiae

Autor: Natalia Kan, Alice-Corina Ceacareanu, Kirill Alexandrov, Anca Niculae, Vadim Sidorovitch

Publikováno v: Protein Expression and Purification. 26:50-58

Rab GTPases are post-translationally geranylgeranylated on their C-terminal cysteines by Rab geranylgeranyl transferase (RabGGTase) and this modification is essential for their biological activity. Rab Escort Protein (REP) is a molecular chaperone th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2af62c1bb56986e3e6097c81c39265d7
https://doi.org/10.1016/s1046-5928(02)00506-5

Double Prenylation by RabGGTase Can Proceed without Dissociation of the Mono-prenylated Intermediate

Autor: Kirill Alexandrov, Anca Niculae, Roger S. Goody, Nicolas H. Thomä

Publikováno v: Journal of Biological Chemistry. 276:48631-48636

Rab geranylgeranyltransferase (RabGGTase) catalyzes the prenylation of Rab proteins. Despite possessing a single active site, RabGGTase is able to add geranylgeranyl moieties onto each of the two C-terminal cysteine residues of Rab. We have studied t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b865fda39492d7abeeb5f2185b5f2c4e
https://doi.org/10.1074/jbc.m106470200

A genetically encodable microtag for chemo-enzymatic derivatization and purification of recombinant proteins

Autor: Herbert Waldmann, Roger S. Goody, Reinhard Reents, Anca Niculae, Reinhard Breitling, Kirill Alexandrov, Alexei Veligodsky, Konstantin Pyatkov, Beatrice-Elena Dursina

Publikováno v: Protein expression and purification. 39(1)

Efficient separation of recombinant polypeptides from proteins of the expression host and their subsequent derivatisation with functional chemical groups is essential for the success of many biological applications. Numerous tag systems have been dev

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b36ce53ef43339f64d23e91e1f3beefd
https://pubmed.ncbi.nlm.nih.gov/15596362

Structure of Rab escort protein-1 in complex with Rab geranylgeranyltransferase

Autor: Herbert Waldmann, Roger S. Goody, Kirill Alexandrov, Vadim Sidorovitch, Ilme Schlichting, Nicolas H. Thomä, Maria−Daniela Cioaca, Ekaterina Bessolitsyna, Reinhard Reents, Olena Pylypenko, Anca Niculae, Alexey Rak

Publikováno v: Molecular Cell
Molecular Cell, 2003, 11 (2), pp.483-494. ⟨10.1016/s1097-2765(03)00044-3⟩
Molecular Cell, Elsevier, 2003, 11 (2), pp.483-494. ⟨10.1016/s1097-2765(03)00044-3⟩

Free to read at publisher's site. Posttranslational geranylgeranylation of Rab GTPases is catalyzed by Rab geranylgeranyltransferase (RabGGTase), which consists of a catalytic α/β heterodimer and an accessory Rab escort protein (REP). The crystal s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c88877bbef92fe973652440380997b62
https://hal.science/hal-02349779