Zobrazeno 1 - 10
of 19
pro vyhledávání: '"Anastassia A. Vorobieva"'
Autor:
Jason C. Klima, Lindsey A. Doyle, Justin Daho Lee, Michael Rappleye, Lauren A. Gagnon, Min Yen Lee, Emilia P. Barros, Anastassia A. Vorobieva, Jiayi Dou, Samantha Bremner, Jacob S. Quon, Cameron M. Chow, Lauren Carter, David L. Mack, Rommie E. Amaro, Joshua C. Vaughan, Andre Berndt, Barry L. Stoddard, David Baker
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-19 (2021)
Fluorescent protein reporters based on GFP exist, but have intrinsic disadvantages. Here the authors incorporate pH, Ca2+ and protein–protein interaction sensing modalities into de novo designed mini-fluorescence-activating proteins (mFAPs), with i
Externí odkaz:
https://doaj.org/article/5f1531b0f2b14a3183076ec5a9a3c859
Autor:
Yakov Kipnis, Anissa Ouald Chaib, Anastassia A. Vorobieva, Guangyang Cai, Gabriella Reggiano, Benjamin Basanta, Eshan Kumar, Peer R.E. Mittl, Donald Hilvert, David Baker
Publikováno v:
Protein science : a publication of the Protein Society. 31(11)
While native scaffolds offer a large diversity of shapes and topologies for enzyme engineering, their often unpredictable behavior in response to sequence modification makes de novo generated scaffolds an exciting alternative. Here we explore the cus
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::204fd9138c0e3c632eda3e39e3d8db2e
https://pubmed.ncbi.nlm.nih.gov/36305767
https://pubmed.ncbi.nlm.nih.gov/36305767
Autor:
Samantha Bremner, Lindsey Doyle, Andre Berndt, Jason C. Klima, David L. Mack, Jiayi Dou, Lauren Carter, Justin Daho Lee, Emilia P. Barros, Joshua C. Vaughan, Cameron M. Chow, David Baker, Min Yen Lee, Michael Rappleye, Rommie E. Amaro, Jacob S. Quon, Lauren A. Gagnon, Anastassia A. Vorobieva, Barry L. Stoddard
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-19 (2021)
Nature communications, vol 12, iss 1
Nature Communications
Nature communications, vol 12, iss 1
Nature Communications
Through the efforts of many groups, a wide range of fluorescent protein reporters and sensors based on green fluorescent protein and its relatives have been engineered in recent years. Here we explore the incorporation of sensing modalities into de n
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1fee693e5fb805cc50f72b2096a30e7f
https://doaj.org/article/5f1531b0f2b14a3183076ec5a9a3c859
https://doaj.org/article/5f1531b0f2b14a3183076ec5a9a3c859
Autor:
Anastassia A. Vorobieva
After decades of progress in computational protein design, the design of proteins folding and functioning in lipid membranes appears today as the next frontier. Some notable successes in the de novo design of simplified model membrane protein systems
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8bd25182aaefac531088cf1ac19324f8
https://hdl.handle.net/20.500.14017/1eb95f5a-df1d-481e-a6c9-284965765615
https://hdl.handle.net/20.500.14017/1eb95f5a-df1d-481e-a6c9-284965765615
Autor:
Karen G. Fleming, G. Nasir Khan, Cameron M. Chow, Binyong Liang, Vicki H. Wysocki, Stacey Gerben, David Baker, Anastassia A. Vorobieva, David J. Brockwell, Dagan C. Marx, Alyssa Q. Stiving, Sheena E. Radford, Jim E. Horne, Sophie R. Harvey, Alex Kang, Sinduja Marx, Asim K. Bera, Paul White, Lukas K. Tamm
Publikováno v:
Science
Building a barrel Computational design offers the possibility of making proteins with customized structures and functions. The range of accessible protein scaffolds has expanded with the design of increasingly complex cytoplasmic proteins and, recent
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::74977f475b654dbf64df1af11c7aae6d
https://doi.org/10.1126/science.abc8182
https://doi.org/10.1126/science.abc8182
Autor:
Cameron M. Chow, Jason C. Klima, Jiayi Dou, David Baker, Lauren Carter, Anastassia A. Vorobieva
Bacterial expression and purification of de novo designed mini-fluorescence-activating proteins (mFAPs) are accomplished by genetically fusing mFAP variants to an N-terminal 6xHis tag and optionally to a Tobacco Etch Virus (TEV) protease epitope. The
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7d3bcdc844752e7073e707b48b42ffb7
https://doi.org/10.21203/rs.3.pex-1077/v1
https://doi.org/10.21203/rs.3.pex-1077/v1
Autor:
Asim K. Bera, Vicki H. Wysocki, Sophie R. Harvey, Jim E. Horne, Sheena E. Radford, Stacey Gerben, David Baker, Paul White, Sinduja Marx, David J. Brockwell, Karen G. Fleming, Anastassia A. Vorobieva, Alex Kang, Dagan C. Marx, Lukas K. Tamm, G. Nasir Khan, Cameron M. Chow, Binyong Liang, Alyssa Q. Stiving
The ability of naturally occurring transmembrane β-barrel proteins (TMBs) to spontaneously insert into lipid bilayers and form stable transmembrane pores is a remarkable feat of protein evolution and has been exploited in biotechnology for applicati
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a9bda580e380783cd8143c20373dd36b
https://doi.org/10.1101/2020.10.22.346965
https://doi.org/10.1101/2020.10.22.346965
Autor:
Jesse R. Huang, Cameron M. Chow, David Baker, Anastassia A. Vorobieva, Stacey Gerben, Hwanhee C. Kim, Jonathan M. Craig, Sinduja Marx, Sarah J. Abell, Jens H. Gundlach
Publikováno v:
Biophysical Journal. 118:483a-484a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::309162566a91177110bebf9539e41e64
https://doi.org/10.1016/j.bpj.2019.11.2678
https://doi.org/10.1016/j.bpj.2019.11.2678
Autor:
Jamie M. Schiffer, Anastassia A. Vorobieva, David Baker, Rommie E. Amaro, Jiayi Dou, Emilia P. Barros
Publikováno v:
Journal of chemical theory and computation, vol 15, iss 10
J Chem Theory Comput
J Chem Theory Comput
Custom-designed ligand-binding proteins represent a promising class of macromolecules with exciting applications towards the design of new enzymes or the engineering of antibodies and small-molecule recruited proteins for therapeutic interventions. H
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cede715e593e5740186e815c1f28a744
https://escholarship.org/uc/item/06c8h67w
https://escholarship.org/uc/item/06c8h67w
Autor:
Celine Dutruel, Mark F. Mohamed, Marko Hyvönen, Gerhard Fischer, Bert van Loo, Christopher D. Bayer, Anastassia A. Vorobieva, Florian Hollfelder, Eugene Valkov, Stefanie Jonas
Publikováno v:
Journal of the American Chemical Society
Highly proficient, promiscuous enzymes can be springboards for functional evolution, able to avoid loss of function during adaptation by their capacity to promote multiple reactions. We employ a systematic comparative study of structure, sequence, an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1b6b7d0a0c079788672ebd530638bad8