Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Anastasia Emmanouilidou"'
Autor:
Ida Höijer, Anastasia Emmanouilidou, Rebecka Östlund, Robin van Schendel, Selma Bozorgpana, Marcel Tijsterman, Lars Feuk, Ulf Gyllensten, Marcel den Hoed, Adam Ameur
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-10 (2022)
CRISPR-Cas9 can introduce unintended off-target effects. Here authors show that unintended mutations produced by in vivo of zebrafish can be inherited by their off-spring.
Externí odkaz:
https://doaj.org/article/c9926fdc297c499ead1e48efd9227591
Autor:
Ida Höijer, Josefin Johansson, Sanna Gudmundsson, Chen-Shan Chin, Ignas Bunikis, Susana Häggqvist, Anastasia Emmanouilidou, Maria Wilbe, Marcel den Hoed, Marie-Louise Bondeson, Lars Feuk, Ulf Gyllensten, Adam Ameur
Publikováno v:
Genome Biology, Vol 21, Iss 1, Pp 1-19 (2020)
Abstract Background One ongoing concern about CRISPR-Cas9 genome editing is that unspecific guide RNA (gRNA) binding may induce off-target mutations. However, accurate prediction of CRISPR-Cas9 off-target activity is challenging. Here, we present SMR
Externí odkaz:
https://doaj.org/article/faeeee05357040f48367202efe662786
Autor:
Adam Ameur, Anastasia Emmanouilidou, Rebecka Östlund, Selma Bozorgpana, Robin van Schendel, Ulf Gyllensten, Ida Höijer, Lars Feuk, Marcel den Hoed
To investigate the extent and distribution of unintended mutations induced by CRISPR-Cas9in vivo,we edited the genome of fertilized zebrafish eggs and investigated DNA from >1100 larvae, juvenile and adult fish in the F0 and F1 generations. Four guid
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c88783bfa2e898727b15577d08477946
https://doi.org/10.1101/2021.10.05.463186
https://doi.org/10.1101/2021.10.05.463186
Autor:
Benedikt von der Heyde, Sitaf Jumaa, Marcel den Hoed, Hannah L. Brooke, Eugenia Mazzaferro, Olga Dethlefsen, Harold Snieder, Amin Allalou, Anastasia Emmanouilidou, Silvia Vicenzi, Erik Ingelsson, Adam Ameur, Eco J. C. de Geus, Ida Höijer, Tiffany Klingström
Publikováno v:
Scientific Reports, Vol 10, Iss 1, Pp 1-18 (2020)
Scientific Reports, 10(1):11831. Nature Publishing Group
Scientific Reports
von der Heyde, B, Emmanouilidou, A, Mazzaferro, E, Vicenzi, S, Höijer, I, Klingström, T, Jumaa, S, Dethlefsen, O, Snieder, H, de Geus, E, Ameur, A, Ingelsson, E, Allalou, A, Brooke, H L & den Hoed, M 2020, ' Translating GWAS-identified loci for cardiac rhythm and rate using an in vivo image-and CRISPR/Cas9-based approach ', Scientific Reports, vol. 10, no. 1, 11831, pp. 1-18 . https://doi.org/10.1038/s41598-020-68567-1
Scientific Reports, 10(1):11831, 1-18. Nature Publishing Group
Scientific Reports, 10(1):11831. Nature Publishing Group
Scientific Reports
von der Heyde, B, Emmanouilidou, A, Mazzaferro, E, Vicenzi, S, Höijer, I, Klingström, T, Jumaa, S, Dethlefsen, O, Snieder, H, de Geus, E, Ameur, A, Ingelsson, E, Allalou, A, Brooke, H L & den Hoed, M 2020, ' Translating GWAS-identified loci for cardiac rhythm and rate using an in vivo image-and CRISPR/Cas9-based approach ', Scientific Reports, vol. 10, no. 1, 11831, pp. 1-18 . https://doi.org/10.1038/s41598-020-68567-1
Scientific Reports, 10(1):11831, 1-18. Nature Publishing Group
A meta-analysis of genome-wide association studies (GWAS) identified eight loci that are associated with heart rate variability (HRV), but candidate genes in these loci remain uncharacterized. We developed an image- and CRISPR/Cas9-based pipeline to
Autor:
Susana Häggqvist, Ulf Gyllensten, Marie-Louise Bondeson, Anastasia Emmanouilidou, Sanna Gudmundsson, Adam Ameur, Ida Höijer, Marcel den Hoed, Josefin Johansson, Ignas Bunikis, Chen-Shan Chin, Lars Feuk, Maria Wilbe
Publikováno v:
Genome Biology
Genome Biology, Vol 21, Iss 1, Pp 1-19 (2020)
Genome Biology, Vol 21, Iss 1, Pp 1-19 (2020)
BackgroundOne ongoing concern about CRISPR-Cas9 genome editing is that unspecific guide RNA (gRNA) binding may induce off-target mutations. However, accurate prediction of CRISPR-Cas9 off-target activity is challenging. Here, we present SMRT-OTS and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b9c0f13f6fa0134bf6beceffa5672c5a
http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-428344
http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-428344
Autor:
Manoj Kumar Bandaru, Hannah L. Brooke, Carolina Wählby, Anders Larsson, Eugenia Mazzaferro, Anastasia Emmanouilidou, Erik Ingelsson, Johan Ledin, Tiffany Klingström, Petter Ranefall, Marcel den Hoed, Mauro Masiero, Benedikt von der Heyde, Olga Dethlefsen
BackgroundHundreds of loci have been robustly associated with circulating lipids, atherosclerosis and coronary artery disease; but for most loci the causal genes and mechanisms remain uncharacterized.MethodsWe developed a semi-automated experimental
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::47268f1deac6052b3870905f23b875d4
https://doi.org/10.1101/502674
https://doi.org/10.1101/502674
Autor:
Marcel den Hoed, Tiffany Klingström, Anastasia Emmanouilidou, Sitaf Jumaa, Eco J. C. de Geus, Harold Snieder, Eugenia Mazzaferro, Olga Dethlefsen, Amin Allalou, Erik Ingelsson, Hannah L. Brooke, Silvia Vicenzi, Benedikt von der Heyde
Publikováno v:
bioRxiv. Cold Spring Harbor Laboratory Press
Heyde, B V D, Emmanouilidou, A, Klingström, T, Mazzaferro, E, Vicenzi, S, Jumaa, S, Dethlefsen, O, Snieder, H, Geus, E D, Ingelsson, E, Allalou, A, Brooke, H L & Hoed, M D 2019, ' Translating GWAS-identified loci for cardiac rhythm and rate using an in vivo image-and CRISPR-Cas9-based approach ', bioRxiv . https://doi.org/10.1101/385500
Heyde, B V D, Emmanouilidou, A, Klingström, T, Mazzaferro, E, Vicenzi, S, Jumaa, S, Dethlefsen, O, Snieder, H, Geus, E D, Ingelsson, E, Allalou, A, Brooke, H L & Hoed, M D 2019, ' Translating GWAS-identified loci for cardiac rhythm and rate using an in vivo image-and CRISPR-Cas9-based approach ', bioRxiv . https://doi.org/10.1101/385500
A meta-analysis of genome-wide association studies (GWAS) identified eight loci that are associated with heart rate variability (HRV), but candidate genes in these loci remain uncharacterized. We developed an image- and CRISPR/Cas9-based pipeline to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a333438d4db147005d0b02537adff45
https://doi.org/10.1101/385500
https://doi.org/10.1101/385500
Autor:
Marcel den Hoed, Amin Allalou, Manoj Kumar Bandaru, Hannah L. Brooke, Erik Ingelsson, Carolina Wählby, Benedikt von der Heyde, Petter Ranefall, Anders Larsson, Anastasia Emmanouilidou
Publikováno v:
Arteriosclerosis, Thrombosis, and Vascular Biology. 38
Background: Genome-wide association (GWAS) have identified hundreds of loci that are robustly associated with the risk of cardiometabolic risk factors and diseases. With few exceptions, the causal genes through which these loci influence disease risk
Autor:
Marcel den Hoed, Tiffany Klingström, Mauro Masiero, Benedikt von der Heyde, Anastasia Emmanouilidou
Publikováno v:
Arteriosclerosis, Thrombosis, and Vascular Biology. 38
Background: High triglyceride levels are an established risk factor for coronary artery disease (CAD). Unpublished results from our proof-of-principle studies show that triglyceride-levels are also associated with atherosclerosis in zebrafish larvae.
Autor:
Anastasia Emmanouilidou, Carolina Wählby, Lisa Conrad, Erik Ingelsson, Anders Larsson, Petter Ranefall, Manoj Kumar Bandaru, Marcel den Hoed
Publikováno v:
Arteriosclerosis, Thrombosis, and Vascular Biology. 36
Genome-wide association studies (GWAS) identified 56 loci that are robustly associated with the risk of coronary artery disease (CAD). Only a handful of genes in these loci are currently known to influence CAD. We previously showed that: 1) CAD-assoc