The pH‐Induced Selectivity Between Cysteine or Histidine Coordinated Heme in an Artificial α‐Helical Metalloprotein

Autor: Anabella Ivancich, Olga Iranzo, Elisabeth Lojou, Barbara Schoepp-Cothenet, Karl J. Koebke, Borries Demeler, Vincent L. Pecoraro, Toni Kühl

Publikováno v: Angewandte Chemie International Edition
Angewandte Chemie International Edition, Wiley-VCH Verlag, 2021, 60 (8), pp.3974-3978. ⟨10.1002/anie.202012673⟩
Angewandte Chemie International Edition, Wiley-VCH Verlag, 2020, ⟨10.1002/anie.202012673⟩
Angewandte Chemie International Edition, Wiley-VCH Verlag, 2020, 60 (8), pp.3974-3978. ⟨10.1002/anie.202012673⟩
Angew Chem Int Ed Engl
Angewandte Chemie International Edition, 2021, 60 (8), pp.3974-3978. ⟨10.1002/anie.202012673⟩

International audience; De Novo metalloprotein design assesses the relationship between metal active site architecture and catalytic reactivity. Herein, we use an alpha-helical scaffold to control the iron coordination geometry when a heme cofactor i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::075e26d97a85efd80fb3eb738b10f55c
https://doi.org/10.1002/ange.202012673

Electron Transfer in Catalases and Catalase-Peroxidases

Autor: Anabella Ivancich, Peter C. Loewen

Publikováno v: Encyclopedia of Biophysics ISBN: 9783642359439
Encyclopedia of Biophysics
Encyclopedia of Biophysics, 2018, 978-3-642-35943-9. ⟨10.1007/978-3-642-35943-9_51-1⟩

International audience; Catalases (EC 1.11.1.6) are enzymes that catalyze the disproportionation of hydrogen peroxide into water and molecular oxygen by means of a heme iron or a dimanganese active site. They are crucial metalloproteins regulating th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1fcb6108c6ef2d00e033f219d4ba2316
https://doi.org/10.1007/978-3-642-35943-9_51-1

Identifying the Elusive Sites of Tyrosyl Radicals in Cytochrome c Peroxidase: Implications for Oxidation of Substrates Bound at a Site Remote from the Heme

Autor: Anabella Ivancich, Lynda J. Donald, Nadime Karaduman, Parisa Hosseinzadeh, Yi Lu, Peter C. Loewen, Thomas D. Pfister, Kyle D. Miner

Publikováno v: Biochemistry
Biochemistry, 2014, 53 (23), pp.3781-3789. ⟨10.1021/bi500353p⟩
Biochemistry, American Chemical Society, 2014, 53 (23), pp.3781-3789. ⟨10.1021/bi500353p⟩

International audience; The location of the Trp radical and the catalytic function of the [Fe(IV)═O Trp191•+] intermediate in cytochrome c peroxidase (CcP) are well-established; however, the unambiguous identification of the site(s) for the forma

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::962d77cd7978150412a8f56f3b04057c
https://doi.org/10.1021/bi500353p

Unprecedented Peroxidase-like Activity of Rhodnius prolixus Nitrophorin 2: Identification of the [FeIV═O Por•]+ and [FeIV═O Por](Tyr38•) Intermediates and Their Role(s) in Substrate Oxidation

Autor: Hongjun Zhang, Fei Yang, F. Ann Walker, Robert E. Berry, Anabella Ivancich, Rahul Singh

Publikováno v: Biochemistry. 49:8857-8872

We have identified a novel enzymatic reaction for nitrophorin 2 (NP2), a heme protein previously characterized as a nitric oxide carrier in the saliva of the Rhodnius prolixus insect. NP2 exhibited levels of peroxidase activity comparable to those of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8b745bf17a24d2b3c2ca722d1b301382
https://doi.org/10.1021/bi100499a

Intramolecular Electron Transfer versus Substrate Oxidation in Lactoperoxidase: Investigation of Radical Intermediates by Stopped-Flow Absorption Spectrophotometry and (9−285 GHz) Electron Paramagnetic Resonance Spectroscopy

Autor: Elena M. Ghibaudi, Anabella Ivancich, Alistair J. Fielding, Barbara Boscolo, Peter C. Loewen, Rahul Singh

Publikováno v: Biochemistry. 47:9781-9792

We have combined the information obtained from rapid-scan electronic absorption spectrophotometry and multifrequency (9-295 GHz) electron paramagnetic resonance (EPR) spectroscopy to unequivocally determine the electronic nature of the intermediates

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::52022b35fd58e302ff2c91b6cf9176bb
https://doi.org/10.1021/bi801032k