Conformation-Dependent High-Affinity Monoclonal Antibodies to Prion Proteins

Autor: Azucena Lemus, Stephen J. DeArmond, Jiri G. Safar, Robert Hnasko, Stanley B. Prusiner, Ana Serban, Larry H. Stanker, Elisa Cleveland

Publikováno v: The Journal of Immunology. 185:729-737

Prion diseases are fatal, neurodegenerative illnesses caused by the accumulation of PrPSc, an aberrantly folded isoform of the normal, cellular prion protein. Detection of PrPSc commonly relies on immunochemical methods, a strategy hampered by the la

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::d308e177d64fbefe558e8dd5b52e5f92
https://doi.org/10.4049/jimmunol.0902930

Modulation of Creutzfeldt-Jakob disease prion propagation by the A224V mutation

Autor: Joel C, Watts, Kurt, Giles, Ana, Serban, Smita, Patel, Abby, Oehler, Sumita, Bhardwaj, Shenheng, Guan, Michael D, Greicius, Bruce L, Miller, Stephen J, DeArmond, Michael D, Geschwind, Stanley B, Prusiner

Publikováno v: Annals of neurology. 78(4)

Mutations in the gene encoding the prion protein (PrP) are responsible for approximately 10 to 15% of cases of prion disease in humans, including Creutzfeldt-Jakob disease (CJD). Here, we report on the discovery of a previously unreported C-terminal

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::2c1add7801f33745da087d5bc9cfc86e
https://pubmed.ncbi.nlm.nih.gov/26094969

Immunoglobulins in Urine of Hamsters with Scrapie

Autor: Ana Serban, Nadia Kovaleva, Kirk C. Hansen, Stanley B. Prusiner, Giuseppe Legname

Publikováno v: Journal of Biological Chemistry. 279:48817-48820

In the prion diseases, a prolonged, asymptomatic incubation period precedes the onset of neurologic dysfunction. At present, a noninvasive test is not available for the presymptomatic diagnosis of prion disease, and thus the report of a test for prio

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1f5c797ae004f6e1c227e8229203c367
https://doi.org/10.1074/jbc.m409107200

Conserved properties of human and bovine prion strains on transmission to guinea pigs

Autor: Jiri G. Safar, Stanley B. Prusiner, Smita S. Patel, Frederic Letessier, Ana Serban, Pierre Lessard, Kurt Giles, Stephen J. DeArmond

Publikováno v: Laboratory investigation; a journal of technical methods and pathology
Safar, JG; Giles, K; Lessard, P; Letessier, F; Patel, S; Serban, A; et al.(2011). Conserved properties of human and bovine prion strains on transmission to guinea pigs. Laboratory Investigation, 91(9), 1326-1336. doi: 10.1038/labinvest.2011.89. UCSF: Retrieved from: http://www.escholarship.org/uc/item/6mf1k484
Laboratory investigation; a journal of technical methods and pathology, vol 91, iss 9

The first transmissions of human prion diseases to rodents used guinea pigs (Gps, Cavia porcellus). Later, transgenic mice expressing human or chimeric human/mouse PrP replaced Gps, but the small size of the mouse limits some investigations. To inves

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9f94dcc8cfaea7276a329ca6923acd97
https://pubmed.ncbi.nlm.nih.gov/21727894

Generation of antisera to purified prions in lipid rafts

Autor: Larry H. Stanker, Ana Serban, George A. Carlson, Robert Hnasko, Stanley B. Prusiner

Prion diseases are fatal neurodegenerative disorders caused by prion proteins (PrP). Infectious prions accumulate in the brain through a template-mediated conformational conversion of endogenous PrP(C) into alternately folded PrP(Sc). Immunoassays to

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f7e6fbfdc19e9495ec151b26781f8b5e
https://europepmc.org/articles/PMC2933057/