Výsledky vyhledávání - "Ana Ruiz Manzano"

High-throughput, fluorescent-aptamer-based measurements of steady-state transcription rates forMycobacterium tuberculosisRNA polymerase

Autor: Drake Jensen, Ana Ruiz Manzano, Maxwell Rector, Eric J. Tomko, M. Thomas Record, Eric A. Galburt

Publikováno v: bioRxiv

The first step in gene expression is the transcription of DNA sequences into RNA. Regulation at the level of transcription leads to changes in steady-state concentrations of RNA transcripts, affecting the flux of downstream functions and ultimately c

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fdd30cc58fb16fe5ea00fa27145e8d38
https://doi.org/10.1101/2023.03.13.532464

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Mycobacterium tuberculosis DNA repair helicase UvrD1 is activated by redox-dependent dimerization via a 2B domain cysteine

Autor: Ankita Chadda, Drake Jensen, Eric J. Tomko, Ana Ruiz Manzano, Binh Nguyen, Timothy M. Lohman, Eric A. Galburt

Publikováno v: Proceedings of the National Academy of Sciences. 119

Significance Mycobacterium tuberculosis ( Mtb ) is an intracellular pathogen that causes tuberculosis and is exposed to oxidative insults from immune system macrophages. Mtb UvrD1 plays a role in DNA repair during infection and has been suggested to

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::8829f899785e925ee60f1e9377ec05b7
https://doi.org/10.1073/pnas.2114501119

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Molecular dissection of RbpA-mediated regulation of fidaxomicin sensitivity in mycobacteria

Autor: Jerome Prusa, Dennis X. Zhu, Aidan J. Flynn, Drake Jensen, Ana Ruiz Manzano, Eric A. Galburt, Christina L. Stallings

Publikováno v: The Journal of biological chemistry. 298(4)

RNA polymerase (RNAP) binding protein A (RbpA) is essential for mycobacterial viability and regulates transcription initiation by increasing the stability of the RNAP-promoter open complex (RP

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3af91898cf70a2df5789e1ef4c382cf7
https://pubmed.ncbi.nlm.nih.gov/35189142

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CarD and RbpA modify the kinetics of initial transcription and slow promoter escape of the Mycobacterium tuberculosis RNA polymerase

Autor: Drake Jensen, Jayan Rammohan, Ana Ruiz Manzano, Christina L. Stallings, Eric A. Galburt

Publikováno v: Nucleic Acids Research

The pathogen Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis, enacts unique transcriptional regulatory mechanisms when subjected to host-derived stresses. Initiation of transcription by the Mycobacterial RNA polymerase (RNAP) ha

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::49d56065bd0e3cf432c3fd9538a75923
https://doi.org/10.1093/nar/gkz449

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Domains within RbpA Serve Specific Functional Roles That Regulate the Expression of Distinct Mycobacterial Gene Subsets

Autor: Ashley L. Garner, Drake Jensen, Steven S. Pope, Justin J. Miller, Ana Ruiz Manzano, Eric A. Galburt, Christina L. Stallings, Jerome Prusa, Gustavo Santiago-Collazo

Publikováno v: Journal of Bacteriology. 200

The RNA polymerase (RNAP) binding protein A (RbpA) contributes to the formation of stable RNAP-promoter open complexes (RP o ) and is essential for viability in mycobacteria. Four domains have been identified in the RbpA protein, i.e., an N-terminal

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::98fbffdf0e350ea1bbe7f05ac5d2f3a9
https://doi.org/10.1128/jb.00690-17

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CarD stabilizes mycobacterial open complexes via a two-tiered kinetic mechanism

Autor: Ana Ruiz Manzano, Christina L. Stallings, Eric A. Galburt, Jayan Rammohan, Ashley L. Garner

Publikováno v: Nucleic Acids Research

CarD is an essential and global transcriptional regulator in mycobacteria. While its biological role is unclear, CarD functions by interacting directly with RNA polymerase (RNAP) holoenzyme promoter complexes. Here, using a fluorescent reporter of op

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::30c055ed5b2081f9c0e86e5e33849cda
https://doi.org/10.1093/nar/gkv078

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CarD integrates three functional modules to promote efficient transcription, antibiotic tolerance, and pathogenesis in mycobacteria

Autor: Ashley L. Garner, Leslie A. Weiss, Eric A. Galburt, Christina L. Stallings, Ana Ruiz Manzano

Publikováno v: Molecular Microbiology. 93:682-697

Summary Although the basic mechanisms of prokaryotic transcription are conserved, it has become evident that some bacteria require additional factors to allow for efficient gene transcription. CarD is an RNA polymerase (RNAP)-binding protein conserve

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::e7f57b81a434afd2c6eef1a2f351f102
https://doi.org/10.1111/mmi.12681

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