Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Ana Preller"'
Publikováno v:
Scanning. 16:111-117
Reflection confocal microscopy was used to determine the intracellular distribution of Type I and III isozymes of hexokinase (ATP:D-hexose 6-phosphotransferase, EC 2.7.1.1) in PC12 cells; detection was by staining with diaminobenzidine as a substrate
Publikováno v:
FEBS Letters. 581:663-666
Besides the classic direct route, frog oocytes incorporate glucosyl units into glycogen by the so-called indirect pathway. The operation of both pathways depends on glucose availability. Below 0.5 mM glucose (calculated intracellular concentration),
Autor:
Susana Peralta, Andres J. Kreiner, Ana Preller, Teresa M. Fonovich de Schroeder, A.A. Burlon, Marcelo D Carattino, Cristina Pérez-Coll, Fabián Naab
Publikováno v:
Ecotoxicology and Environmental Safety. 57:311-318
The effects of copper and cadmium on metabolism through the pentose phosphate pathway were evaluated in Bufo arenarum toad ovary. The effects of the two metals on dehydrogenases from this pathway were evaluated by three experiments: (1) in samples ob
Publikováno v:
Archives of Biochemistry and Biophysics. 413:9-16
Frog oocyte glycogen synthase properties differ significantly under in vitro or in vivo conditions. The K(mapp) for UDP-glucose in vivo was 1.4mM (in the presence or absence of glucose-6-P). The in vitro value was 6mM and was reduced by glucose-6-P t
Publikováno v:
IUBMB Life (International Union of Biochemistry and Molecular Biology: Life). 51:5-10
This review is intended to illustrate how live frog oocytes may be advantageously used to address the study of some problems of in vivo glucose metabolism. Glucose microinjected into the cells is preferentially committed to glycogen synthesis. We pre
Publikováno v:
FEBS Letters. 446:149-152
Evolution of CO2 from labelled glucose microinjected into frog oocytes in vivo may be ascribed to the pentose-P pathway, as measured by radioactive CO2 production from [1-14C] and [6-14C]glucose. Coinjection of NADP+ and [14C]glucose significantly st
Publikováno v:
Electrophoresis. 28(16)
Glycogen synthase catalyzes the incorporation of UDP-glucose into glycogen. The activity of the enzyme is usually measured either by a spectrophotometric method or by a radioassay. The first one is not suitable because of the difficulties regarding t
Publikováno v:
Archives of biochemistry and biophysics. 348(1)
Glycogen synthesis following glucose microinjection in frog oocytes proceeds preferentially by an indirect pathway involving gluconeogenesis from triose compounds. Because of the known regulatory role of fructose-2,6-bisP on glucose utilization in mo
Publikováno v:
The Biochemical journal. 315
Glycogen is the main end product of glucose metabolism in amphibian oocytes. However, in the first few minutes after [U-14C]glucose microinjection most of the label is found in lactate. The burst of lactate production and the shape of the time curves
Publikováno v:
Archives of biochemistry and biophysics. 316(1)
The characterization of fructose-1,6-bisphosphatase in stage VI oocytes from the frog Caudiverbera caudiverbera, as well as the in vivo activity, is reported. The enzyme has a subunit molecular weight of approximately 43,500, has an apparent Km value