Crosslinking assay to study a specific cargo-coat interaction through a transmembrane receptor in the secretory pathway.

Autor: Javier Manzano-Lopez, Sofia Rodriguez-Gallardo, Susana Sabido-Bozo, Alejandro Cortes-Gomez, Ana Maria Perez-Linero, Rafael Lucena, Antonio Cordones-Romero, Sergio Lopez, Auxiliadora Aguilera-Romero, Manuel Muñiz

Publikováno v: PLoS ONE, Vol 17, Iss 2, p e0263617 (2022)

Intracellular trafficking through the secretory organelles depends on transient interactions between cargo proteins and transport machinery. Cytosolic coat protein complexes capture specific luminal cargo proteins for incorporation into transport ves

Externí odkaz: https://doaj.org/article/11b36763662c4d44a1606d0ec1aa449c

Determination of the lipid composition of the GPI anchor.

Autor: Auxiliadora Aguilera-Romero, Susana Sabido-Bozo, Sergio Lopez, Alejandro Cortes-Gomez, Sofia Rodriguez-Gallardo, Ana Maria Perez-Linero, Isabelle Riezman, Howard Riezman, Manuel Muñiz

Publikováno v: PLoS ONE, Vol 16, Iss 8, p e0256184 (2021)

In eukaryotic cells, a subset of cell surface proteins is attached by the glycolipid glycosylphosphatidylinositol (GPI) to the external leaflet of the plasma membrane where they play important roles as enzymes, receptors, or adhesion molecules. Here

Externí odkaz: https://doaj.org/article/2907df15772340d8b321990edcd67aef

Dual Independent Roles of the p24 Complex in Selectivity of Secretory Cargo Export from the Endoplasmic Reticulum

Autor: Sergio Lopez, Ana Maria Perez-Linero, Javier Manzano-Lopez, Susana Sabido-Bozo, Alejandro Cortes-Gomez, Sofia Rodriguez-Gallardo, Auxiliadora Aguilera-Romero, Veit Goder, Manuel Muñiz

Publikováno v: Cells, Vol 9, Iss 5, p 1295 (2020)

The cellular mechanisms that ensure the selectivity and fidelity of secretory cargo protein transport from the endoplasmic reticulum (ER) to the Golgi are still not well understood. The p24 protein complex acts as a specific cargo receptor for GPI-an

Externí odkaz: https://doaj.org/article/0cf7c4ad0cea4e23b7fdbd1283e94285

The p24 Complex Contributes to Specify Arf1 for COPI Coat Selection

Autor: Javier Manzano-López, Manuel Muñiz, Sergio López, Ralf Erik Wellinger, Ana Maria Perez-Linero, Alejandro Cortes-Gomez, Susana Sabido-Bozo, Sofia Rodriguez-Gallardo, Auxiliadora Aguilera-Romero

Publikováno v: International Journal of Molecular Sciences, Vol 22, Iss 423, p 423 (2021)
International Journal of Molecular Sciences
Volume 22
Issue 1
idUS. Depósito de Investigación de la Universidad de Sevilla
instname

Golgi trafficking depends on the small GTPase Arf1 which, upon activation, drives the assembly of different coats onto budding vesicles. Two related types of guanine nucleotide exchange factors (GEFs) activate Arf1 at different Golgi sites. In yeast,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ec2479e01b4a68af7e1f0d37050e423d
https://www.mdpi.com/1422-0067/22/1/423

Assay for dual cargo sorting into endoplasmic reticulum exit sites imaged by 3D Super-resolution Confocal Live Imaging Microscopy (SCLIM)

Autor: Miho Waga, Akihiko Nakano, Auxiliadora Aguilera-Romero, Alejandro Cortes-Gomez, Sergio López, Sofia Rodriguez-Gallardo, Manuel Muñiz, Kazuo Kurokawa, Susana Sabido-Bozo, Ana Maria Perez-Linero

Publikováno v: PLoS ONE
PLoS ONE, Vol 16, Iss 10, p e0258111 (2021)
idUS. Depósito de Investigación de la Universidad de Sevilla
instname
Digital.CSIC. Repositorio Institucional del CSIC

Understanding how in eukaryotic cells thousands of proteins are sorted from each other through the secretory pathway and delivered to their correct destinations is a central issue of cell biology. We have further investigated in yeast how two distinc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b59320712b058d18e251534ae6e08dd3
https://hdl.handle.net/10668/3822