Výsledky vyhledávání - "Ana María Guadalajara"

Molecular characterization of carbamoyl-phosphate synthetase (CPS1) deficiency using human recombinant CPS1 as a key tool

Autor: Isabel Pérez-Arellano, Vicente Rubio, Ana María Guadalajara, Belén Barcelona, Marshall L. Summar, Ana I. Martínez, Javier Cervera, Carmen Diez-Fernandez, Satu Pekkala

Publikováno v: HUMAN MUTATION
r-CIPF: Repositorio Institucional Producción Científica del Centro de Investigación Principe Felipe (CIPF)
Centro de Investigación Principe Felipe (CIPF)
Digital.CSIC. Repositorio Institucional del CSIC
instname
r-CIPF. Repositorio Institucional Producción Científica del Centro de Investigación Principe Felipe (CIPF)

11 páginas, 5 figuras, 1 tabla. El fichero contiene el material suplementario.
The urea cycle disease carbamoyl-phosphate synthetase deficiency (CPS1D) has been associated with many mutations in the CPS1 gene [Häberle et al., 2011. Hum Mutat 3

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f97d128ad7d1fab841bdf7ea5d97b8f6
https://doi.org/10.1002/humu.22349

Zobrazit plný text záznamu

Physical location of the site for N-acetyl-L-glutamate, the allosteric activator of carbamoyl phosphate synthetase, in the 20-kilodalton COOH-terminal domain

Autor: Vicente Rubio, Leandro B. Rodriguez-Aparicio, Ana María Guadalajara

Publikováno v: Biochemistry. 28(7)

Mammalian liver mitochondrial carbamoyl phosphate synthetase, a polypeptide of 160 kDa, is activated allosterically by N-acetyl-L-glutamate. The analogue of this activator N-(chloroacetyl)-L-[14C]glutamate has been found to serve as a photoaffinity l

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::66568def2da6e48780b6263f507af242
https://pubmed.ncbi.nlm.nih.gov/2742825

Zobrazit plný text záznamu

Limited proteolysis reveals low-affinity binding of N-acetyl-L-glutamate to rat-liver carbamoyl-phosphate synthetase (ammonia)

Autor: Vicente Rubio, Ana María Guadalajara, Santiago Grisolia

Publikováno v: Scopus-Elsevier

Carbamoyl-phosphate synthetase was inactivated by elastase with first-order kinetics, and N-acetyl-L-glutamate speeded inactivation. From the dependence of the t1/2 value for inactivation on the concentration of acetylglutamate we estimate a Kd value

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::193c2dd6c3028ee067ba1eb5d67cd6a9
https://pubmed.ncbi.nlm.nih.gov/3646105

Zobrazit plný text záznamu

Inactivation of carbamoyl phosphate synthetase (ammonia) by elastase as a probe to investigate binding of the substrates

Autor: Ana María Guadalajara, Vicente Rubio, Santiago Grisolia

Publikováno v: Scopus-Elsevier

Rat liver carbamoyl phosphate synthetase I is inactivated by elastase. Addition of ATP, Mg2+, K+ and N-acetyl-L-glutamate (the physiological allosteric activator) protects entirely, whereas acetylglutamate alone speeds inactivation. We have exploited

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d9c17aef7224d6e4dc65e83bcc9dbdb6
http://www.scopus.com/inward/record.url?eid=2-s2.0-0021116096&partnerID=MN8TOARS

Zobrazit plný text záznamu

Vyhledávací nástroje:

Upřesnit hledání