Výsledky vyhledávání - "Ana J. Vecino"

The transmembrane domain of the T4SS coupling protein TrwB and its role in protein–protein interactions

Autor: Rosa L. Segura, Begoña Ugarte-Uribe, Ana J. Vecino, Félix M. Goñi, Itziar Alkorta, Fernando de la Cruz, Sandra Águila-Arcos

Publikováno v: Biochimica et Biophysica Acta (BBA)-Biomembranes; Vol 1828
Digital.CSIC. Repositorio Institucional del CSIC
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Bacteria use type IV secretion systems to transfer genetic material and proteins from donor to recipient cells, using proteins encoded by conjugative plasmids. Among those proteins the so-called Type IV Coupling Protein plays a central role in the pr

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Deletion of a single helix from the transmembrane domain causes large changes in membrane insertion properties and secondary structure of the bacterial conjugation protein TrwB

Autor: Igor de la Arada, Rosa L. Segura, Ana J. Vecino, Félix M. Goñi, Itziar Alkorta, José Luis R. Arrondo, Fernando de la Cruz

Publikováno v: Digital.CSIC. Repositorio Institucional del CSIC
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TrwB is an essential protein in the conjugative transfer of plasmid R388. The protein consists of a bulky cytosolic domain containing the catalytic site, and a small transmembrane domain (TMD). Our previous studies support the idea that the TMD plays

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Membrane insertion stabilizes the structure of TrwB, the R388 conjugative plasmid coupling protein

Autor: Ana J. Vecino, Igor de la Arada, Félix M. Goñi, Itziar Alkorta, José Luis R. Arrondo, Fernando de la Cruz, Rosa L. Segura

Publikováno v: Digital.CSIC. Repositorio Institucional del CSIC
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TrwB is an integral membrane protein that plays a crucial role in the conjugative process of plasmid R388. We have recently shown [Vecino et al., Biochim. Biophys. Acta 1798(11), 2160-2169 (2010)] that TrwB can be reconstituted into liposomes, and th

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Reconstitution in liposome bilayers enhances nucleotide binding affinity and ATP-specificity of TrwB conjugative coupling protein

Autor: Begoña Ugarte-Uribe, Itsaso Hormaeche, Itziar Alkorta, Félix M. Goñi, Rosa L. Segura, Ana J. Vecino, Fernando de la Cruz, Sandra Águila

Publikováno v: Digital.CSIC. Repositorio Institucional del CSIC
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Bacterial conjugative systems code for an essential membrane protein that couples the relaxosome to the DNA transport apparatus, called type IV couplingprotein (T4CP). TrwB is the T4CP of the conjugative plasmid R388. In earlier work we found that th

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Subcellular location of the coupling protein TrwB and the role of its transmembrane domain

Autor: Ana J. Vecino, Rosa L. Segura, Félix M. Goñi, Sandra Águila-Arcos, Itziar Alkorta, Fernando de la Cruz, Begoña Ugarte-Uribe

Publikováno v: Digital.CSIC. Repositorio Institucional del CSIC
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Conjugation is the most important mechanism for horizontal gene transfer and it is the main responsible for the successful adaptation of bacteria to the environment. Conjugative plasmids are the DNA molecules transferred and a multiprotein system enc

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http://hdl.handle.net/10261/130525

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Membrane insertion stabilizes TrwB, the coupling protein of the conjugative plasmid R388

Autor: Itziar Alkorta, Félix M. Goñi, Rosa L. Segura, Ana J. Vecino, Fernando de la Cruz, José Luis R. Arrondo, Igor de la Arada

Publikováno v: Chemistry and Physics of Lipids. 163:S47

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::d7a9c5bad96413ec6c61a48251119c4e
https://doi.org/10.1016/j.chemphyslip.2010.05.142

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Reconstitution into liposomes enhances nucleotide binding affinity of TrwB conjugative coupling protein

Autor: Félix M. Goñi, Ana J. Vecino, Itziar Alkorta, Sandra Águila, Rosa L. Segura, Begoña Ugarte-Uribe, Fernando de la Cruz

Publikováno v: Chemistry and Physics of Lipids. 163:S48

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https://doi.org/10.1016/j.chemphyslip.2010.05.143

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The transmembrane domain provides nucleotide binding specificity to the bacterial conjugation protein TrwB

Autor: Fernando de la Cruz, Itsaso Hormaeche, Itziar Alkorta, Félix M. Goñi, Ana J. Vecino, Rosa L. Segura

Publikováno v: FEBS Letters. (13):3075-3082

In order to understand the functional significance of the transmembrane domain of TrwB, an integral membrane protein involved in bacterial conjugation, the protein was purified in the native, and also as a truncated soluble form (TrwBΔN70). The inta

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