A Venomics Approach Coupled to High-Throughput Toxin Production Strategies Identifies the First Venom-Derived Melanocortin Receptor Agonists

Autor: Miguel Cabo Diez, Edwin De Pauw, Ricardo C. Rodríguez de la Vega, Victoria Fernández Pedrosa, Rebeca Miñambres Herráiz, Denis Servent, Steve Reynaud, Ana Filipa Sequeira, Renaud Vincentelli, Céline Landon, Rudy Fourmy, Joana L. A. Brás, Hervé Meudal, Yoan Duhoo, Margot Vanden Driessche, Annette G. Beck-Sickinger, Gilles Mourier, Justyna Ciolek, Oscar Ramos, Carlos M. G. A. Fontes, Natalie J. Saez, Aude Violette, Michel Degueldre, Loïc Quinton, Karin Mörl, Marion Verdenaud, Julia Boeri, N. Gilles, Frédéric Ducancel, Steve Peigneur, Jan Tytgat, Gregory Upert

Publikováno v: Journal of Medicinal Chemistry
Journal of Medicinal Chemistry, American Chemical Society, 2020, 63 (15), pp.8250-8264. ⟨10.1021/acs.jmedchem.0c00485⟩
Journal of Medicinal Chemistry, 2020, 63 (15), pp.8250-8264. ⟨10.1021/acs.jmedchem.0c00485⟩

International audience; Animal venoms are rich in hundreds of toxins with extraordinary biological activities. Their exploitation is difficult due to their complexity and the small quantities of venom available from most venomous species. We develope

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::451cebc38e07bcea301b752897b6406f
https://hal.archives-ouvertes.fr/hal-02936788/document

The Fine Structure of the Cellulosome Defines the Intricacies of Carbohydrate Deconstruction in the Mammalian Gut

Autor: Shabir Najmudin, Joana L. A. Brás, Victor D. Alves, A. Leitão, Pedro Bule, Ana Filipa Sequeira, José A. M. Prates, Vania O. Fernandes, Luís M. A. Ferreira, Kate Cameron, Carlos M. G. A. Fontes, Virgínia M. R. Pires

Publikováno v: Advances in Animal Health, Medicine and Production ISBN: 9783030619800

Protein-protein interactions play a vital role in many cellular processes. One of the most notable examples is the assembly of the cellulosome, a bacterial multi-enzyme complex that efficiently degrades cellulose and hemicellulose. Cellulosome assemb

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::084195c85acbe21221ae141ed016adf7
https://doi.org/10.1007/978-3-030-61981-7_5

High-Throughput Production of a New Library of Human Single and Tandem PDZ Domains Allows Quantitative PDZ-Peptide Interaction Screening Through High-Throughput Holdup Assay

Autor: Laurie Ramond, Nicolas Wolff, Gilles Travé, Joana L. A. Brás, Carlos M. G. A. Fontes, Ana Filipa Sequeira, Renaud Vincentelli, Vânia Cardoso, Yoan Duhoo, Virginie Girault, Patrick Fourquet, Yves Nominé, Jeremy Turchetto, Fabien Durbesson

Publikováno v: High-Throughput Protein Production and Purification
Renaud Vincentelli. High-Throughput Protein Production and Purification, Springer, pp.439-476, 2019, 978-1-4939-9623-0. ⟨10.1007/978-1-4939-9624-7_21⟩
Methods in Molecular Biology
Methods in Molecular Biology-High-Throughput Protein Production and Purification
Methods in Molecular Biology ISBN: 9781493996230

International audience; PDZ domains recognize PDZ Binding Motifs (PBMs) at the extreme C-terminus of their partner proteins. The human proteome contains 266 identified PDZ domains, the PDZome, spread over 152 proteins. We previously developed the "ho

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::486ddc836510540ecef68070d7f7483b
https://hal.archives-ouvertes.fr/hal-02398097

A Novel Platform for High-Throughput Gene Synthesis to Maximize Recombinant Expression in Escherichia coli

Autor: Ana Filipa, Sequeira, Joana L A, Brás, Vânia O, Fernandes, Catarina I P D, Guerreiro, Renaud, Vincentelli, Carlos M G A, Fontes

Publikováno v: Methods in molecular biology (Clifton, N.J.). 1620

Gene synthesis is becoming an important tool in many fields of recombinant DNA technology, including recombinant protein production. De novo gene synthesis is quickly replacing the classical cloning and mutagenesis procedures and allows generating nu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::e9e92ec36cf3a44ba4815210335bc6ef
https://pubmed.ncbi.nlm.nih.gov/28540703

Development of a gene synthesis platform for the efficient large scale production of small genes encoding animal toxins

Autor: Renaud Vincentelli, Carlos M. G. A. Fontes, Ana Filipa Sequeira, Joana L. A. Brás, Catarina I. P. D. Guerreiro

Publikováno v: BMC Biotechnology
BMC Biotechnology, BioMed Central, 2016, 16 (1), ⟨10.1186/s12896-016-0316-3⟩
BMC Biotechnology, 2016, 16 (1), ⟨10.1186/s12896-016-0316-3⟩

Background Gene synthesis is becoming an important tool in many fields of recombinant DNA technology, including recombinant protein production. De novo gene synthesis is quickly replacing the classical cloning and mutagenesis procedures and allows ge

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1aa44ba40f270acf3565ecd4d8d3f0f2
https://hal.archives-ouvertes.fr/hal-01439102/document