Viral Genome Conformations and Contacts across Different Lifecycle Stages

Autor: Peter G. Stockley, Nikesh Patel, Emma L. Wroblewski, Andrew J. P. Scott, Carlos P. Mata, Rebecca Chandler-Bostock, Amy M. Barker, Reidun Twarock, Sam Clark, Richard J. Bingham

Publikováno v: Proceedings, Vol 50, Iss 1, p 109 (2020)

Single-stranded RNA viral genomes (gRNA) are dynamic molecules that permit packaging into virions and their subsequent extrusion during infection. For viruses with such genomes, we discovered a previously unsuspected mechanism that regulates their as

Externí odkaz: https://doaj.org/article/332fa0bbcc6e48489b6db56ff347f7d7

Can m6A-modified RNA be used as an Anti-Viral Target?

Autor: Richard Foster, Konstantin Röder, Adrian Whitehouse, Amy M. Barker, Samuela Pasquali

Publikováno v: Access Microbiology. 2

Kaposi’s sarcoma-associated herpesvirus (KSHV) is a DNA virus associated with several HIV-associated malignancies. Like all herpesviruses, KSHV has a biphasic life cycle encompassing a latent state and lytic replication. The KSHV replication and tr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::eb64687cf637a79ca2bf80af11bdfd3d
https://doi.org/10.1099/acmi.ac2020.po1035

Mutations of the domain forming the dimeric interface of the ArdA protein affect dimerization and antimodification activity but not antirestriction activity

Autor: David T. F. Dryden, Julia Madrzak, John H. White, Garry W. Blakely, Laurie P. Cooper, Kai Chen, Gareth A. Roberts, Iain W. Manfield, Amy M. Barker, Edward K M Bower, Arcadia Woods

Publikováno v: The Febs Journal
Roberts, G A, Chen, K, Bower, E K M, Madrzak, J, Woods, A, Barker, A M, Cooper, L P, White, J H, Blakely, G W, Manfield, I & Dryden, D T F 2013, ' Mutations of the domain forming the dimeric interface of the ArdA protein affect dimerisation and antimodification activity but not antirestriction activity ', Febs Journal, vol. 280, no. 19, pp. 4903–4914 . https://doi.org/10.1111/febs.12467

ArdA antirestriction proteins are encoded by genes present in many conjugative plasmids and transposons within bacterial genomes. Antirestriction is the ability to prevent cleavage of foreign incoming DNA by restriction-modification (RM) systems. Ant

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::24981f9f211d48933e39945d123ff78e
http://europepmc.org/articles/PMC3906837

Sequence-Specific, RNA–Protein Interactions Overcome Electrostatic Barriers Preventing Assembly of Satellite Tobacco Necrosis Virus Coat Protein

Autor: Arwen R. Pearson, Robert H.A. Coutts, Robert J. Ford, Saskia E. Bakker, Simon E. V. Phillips, Amy M. Barker, Peter G. Stockley, Neil A. Ranson

Publikováno v: Journal of Molecular Biology

We have examined the roles of RNA–coat protein (CP) interactions in the assembly of satellite tobacco necrosis virus (STNV). The viral genomic RNA encodes only the CP, which comprises a β-barrel domain connected to a positively charged N-terminal

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1e564bce8bc03ad1b6b508644b8bee7f

Distinguishing closely related amyloid precursors using an RNA aptamer

Autor: Amy M. Barker, Peter G. Stockley, Gary S. Thompson, Claire J. Sarell, Arnout P. Kalverda, Sheena E. Radford, David H.J. Bunka, Theodoros K. Karamanos, Simon J. White

Publikováno v: The Journal of Biological Chemistry
Journal of Biological Chemistry

Background: Altering the co-polymerization of proteins into amyloid fibrils provides an opportunity for manipulating fibril assembly. Results: NMR and kinetic analysis showed that an RNA aptamer distinguishes between two highly similar co-aggregating

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1ebe8cbb86196a66e12883d264c20580
https://pubmed.ncbi.nlm.nih.gov/25100729