An Adaptable and Modular Set of Laboratory Exercises Connecting Genotype to Phenotype in Sporulating Bacillus subtilis

Autor: Anna L. McLoon, Amy H. Camp, Lilah Rahn-Lee

Publikováno v: Journal of Microbiology & Biology Education, Vol 22, Iss 3 (2021)

ABSTRACT Practical lab exercises that help students draw connections between genotype and phenotype, and make and test predictions about the identity of mutants, are invaluable in college-level cell biology, genetics, and microbiology courses. While

Externí odkaz: https://doaj.org/article/d54ea4552f5b4f26b80005e70ab1c1d5

A novel RNA polymerase‐binding protein that interacts with a sigma‐factor docking site

Autor: Ann Hochschild, Caterina Alfano, Ewelina M. Krysztofinska, Rivka L. Isaacson, Cinthia P. Garcia, Anna F. Wang Erickson, Padraig Deighan, Kelsey Barrasso, Santiago Martínez-Lumbreras, Richard Losick, Arjun Thapaliya, Amy H. Camp, Shanshan Chen

Publikováno v: Wang Erickson, A F, Deighan, P, Chen, S, Barrasso, K, Garcia, C P, Martínez-Lumbreras, S, Alfano, C, Krysztofinska, E M, Thapaliya, A, Camp, A H, Isaacson, R L, Hochschild, A & Losick, R 2017, ' A Novel RNA Polymerase-binding Protein that interacts with a Sigma-Factor Docking Site ', Molecular Microbiology . https://doi.org/10.1111/mmi.13724

Sporulation in Bacillus subtilis is governed by a cascade of alternative RNA polymerase sigma factors. We previously identified a small protein Fin that is produced under the control of the sporulation sigma factor σ(F) to create a negative feedback

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::82a07e2951497dcbc147b45bdebb8c04
https://doi.org/10.1111/mmi.13724

Structural Characterization of SpoIIIAB Sporulation-essential Protein in Bacillus subtilis

Autor: Liam J. Worrall, Kelly A. Flanagan, S.C. Massoni, N. Zeytuni, Natalie C. J. Strynadka, Amy H. Camp

Endospore formation in the Gram-positive bacterium Bacillus subtilis initiates in response to nutrient depletion and involves a series of morphological changes that result in the creation of a dormant spore. Early in this developmental process, the c

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0565368851de3cab1de2db7fbac79263
https://europepmc.org/articles/PMC5864543/

Near-atomic resolution cryoelectron microscopy structure of the 30-fold homooligomeric SpoIIIAG channel essential to spore formation in Bacillus subtilis

Autor: N. Zeytuni, Liam J. Worrall, Marija Vuckovic, Chuan Hong, Shawn C. Massoni, Zhiheng Yu, Natalie C. J. Strynadka, Rick Huang, Kate A. Theiltges, Kelly A. Flanagan, Amy H. Camp

Publikováno v: Proceedings of the National Academy of Sciences. 114

Bacterial sporulation allows starving cells to differentiate into metabolically dormant spores that can survive extreme conditions. Following asymmetric division, the mother cell engulfs the forespore, surrounding it with two bilayer membranes. Durin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::25927dc5f72dbf20a4321170fec43480
https://doi.org/10.1073/pnas.1704310114

Small Genes under Sporulation Control in the Bacillus subtilis genome

Autor: Matthias Schmalisch, Andrea Muffler, Sabrina Rodriguez, John B. Perkins, Richard Losick, Elisa Maiques, Amy H. Camp, Bastien Chevreux, Lachezar A. Nikolov

Publikováno v: Journal of Bacteriology. 192:5402-5412

Using an oligonucleotide microarray, we searched for previously unrecognized transcription units in intergenic regions in the genome of Bacillus subtilis , with an emphasis on identifying small genes activated during spore formation. Nineteen transcr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::96db240dbb6dfea5481ae195f9e2fe34
https://doi.org/10.1128/jb.00534-10