Zobrazeno 1 - 10
of 175
pro vyhledávání: '"Amy E. Herr"'
Mapping of UV-C dose and SARS-CoV-2 viral inactivation across N95 respirators during decontamination
Autor:
Alisha Geldert, Alison Su, Allison W. Roberts, Guillaume Golovkine, Samantha M. Grist, Sarah A. Stanley, Amy E. Herr
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
Abstract During public health crises like the COVID-19 pandemic, ultraviolet-C (UV-C) decontamination of N95 respirators for emergency reuse has been implemented to mitigate shortages. Pathogen photoinactivation efficacy depends critically on UV-C do
Externí odkaz:
https://doaj.org/article/2587540047b34e259e871ab0cec2e1bd
Autor:
Julea Vlassakis, Louise L. Hansen, Ryo Higuchi-Sanabria, Yun Zhou, C. Kimberly Tsui, Andrew Dillin, Haiyan Huang, Amy E. Herr
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-14 (2021)
Existing methods for multimeric protein complex quantification in single cells suffer from limited selectivity and sensitivity. Here the authors report Single-cell protein Interaction Fractionation Through Electrophoresis and immunoassay Readout (SIF
Externí odkaz:
https://doaj.org/article/b415092cbb764619a17faea6a93f2682
Autor:
Ana E. Gomez Martinez, Amy E. Herr
Publikováno v:
ACS Measurement Science Au, Vol 1, Iss 3, Pp 139-146 (2021)
Externí odkaz:
https://doaj.org/article/ddf785767d534109a36a056ef5239fcd
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-17 (2020)
Single-cell immunoblotting previously separated proteins on a polyacrylamide slab in the xy direction and was limited by throughput and sample consumption. Here the authors adapt the system to separate proteins in the z direction, allowing for closer
Externí odkaz:
https://doaj.org/article/2e3c86ebf1d34b6fa32c5f5fcd0d15a1
Autor:
Alden C. Moss, Amy E. Herr
Publikováno v:
APL Bioengineering, Vol 4, Iss 4, Pp 046104-046104-10 (2020)
Fluorescence-based DNA readouts are increasingly important in biological research, owing to enhanced analytical sensitivity and multiplexing capability. In this study, we characterize an in-gel polymerase elongation process to understand the reaction
Externí odkaz:
https://doaj.org/article/106a74b07f314ff2bea72d344cd53b03
Autor:
Elly Sinkala, Elodie Sollier-Christen, Corinne Renier, Elisabet Rosàs-Canyelles, James Che, Kyra Heirich, Todd A. Duncombe, Julea Vlassakis, Kevin A. Yamauchi, Haiyan Huang, Stefanie S. Jeffrey, Amy E. Herr
Publikováno v:
Nature Communications, Vol 8, Iss 1, Pp 1-12 (2017)
Circulating tumour cells (CTCs) are rare cells found in the blood of certain cancer patients. Here, the authors develop a cytometry tool that appends a microfluidic western blot to a CTC isolation workflow and apply it to profile a panel of proteins
Externí odkaz:
https://doaj.org/article/07fbb330fecc4ed28810eb8735b09dd0
Publikováno v:
APL Bioengineering, Vol 3, Iss 2, Pp 026101-026101-10 (2019)
Understanding and controlling molecular transport in hydrogel materials is important for biomedical tools, including engineered tissues and drug delivery, as well as life sciences tools for single-cell analysis. Here, we scrutinize the ability of mic
Externí odkaz:
https://doaj.org/article/3c5709ab427a4443a01fbf88099f3f71
Autor:
James Eberwine, Junhyong Kim, Ron C. Anafi, Steven Brem, Maja Bucan, Stephen A. Fisher, M. Sean Grady, Amy E. Herr, David Issadore, Hyejoong Jeong, HyunBum Kim, Daeyeon Lee, Stanislav Rubakhin, Jai-Yoon Sul, Jonathan V. Sweedler, John A. Wolf, Kenneth S. Zaret, James Zou
Publikováno v:
Nat Methods
We argue that the study of single-cell subcellular organelle omics is needed to understand and regulate cell function. This requires and is being enabled by new technology development.
Publikováno v:
SLAS Technol
New pipelines are required to automate the quantitation of emerging high-throughput electrophoretic (EP) assessment of DNA damage, or proteoform expression in single cells. EP cytometry consists of thousands of Western blots performed on a microscope
Publikováno v:
Photochemistry and Photobiology. 98:864-873
Effective ultraviolet-C (UV-C) decontamination protocols of N95 respirators require validation that the entire N95 surface receives sufficient dose. Photochromic indicators (PCIs) can accurately measure UV-C dose on nonplanar surfaces, but often satu