Zobrazeno 1 - 10
of 201
pro vyhledávání: '"Amy E, Palmer"'
Publikováno v:
STAR Protocols, Vol 5, Iss 2, Pp 103130- (2024)
Summary: Zinc (Zn2+) plays roles in structure, catalysis, and signaling. The majority of cellular Zn2+ is bound by proteins, but a fraction of total Zn2+ exists in a labile form. Here, we present a protocol for measuring labile cytosolic Zn2+ using a
Externí odkaz:
https://doaj.org/article/8a95b34e81c3481b880406a15759bead
Publikováno v:
STAR Protocols, Vol 5, Iss 2, Pp 103122- (2024)
Summary: The exchangeable Zn2+ pool in cells is not static but responds to perturbations as well as fluctuates naturally through the cell cycle. Here, we present a protocol to carry out long-term live-cell imaging of cells expressing a cytosolic Zn2+
Externí odkaz:
https://doaj.org/article/b980e374c32141408b7ff2b55ea5ee4d
Publikováno v:
Scientific Reports, Vol 12, Iss 1, Pp 1-12 (2022)
Abstract The regulation of transcription is a complex process that involves binding of transcription factors (TFs) to specific sequences, recruitment of cofactors and chromatin remodelers, assembly of the pre-initiation complex and recruitment of RNA
Externí odkaz:
https://doaj.org/article/8ba32ab2bf0b49c2944fc031765c7551
Publikováno v:
Cell Reports, Vol 42, Iss 6, Pp 112656- (2023)
Summary: Zinc is an essential micronutrient required for all domains of life. Cells maintain zinc homeostasis using a network of transporters, buffers, and transcription factors. Zinc is required for mammalian cell proliferation, and zinc homeostasis
Externí odkaz:
https://doaj.org/article/e651792fcfc74bc78febb54a3b5a772b
Autor:
Shelby R. Lennon, Aleksandra J. Wierzba, Shea H. Siwik, Dorota Gryko, Amy E. Palmer, Robert T. Batey
Publikováno v:
ACS Chemical Biology. 18:1136-1147
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2342316c381c8a6734920f6323ae7d60
https://doi.org/10.1021/acschembio.2c00939
https://doi.org/10.1021/acschembio.2c00939
Publikováno v:
eLife, Vol 9 (2020)
Zinc is widely recognized as essential for growth and proliferation, yet the mechanisms of how zinc deficiency arrests these processes remain enigmatic. Here we induce subtle zinc perturbations and track asynchronously cycling cells throughout divisi
Externí odkaz:
https://doaj.org/article/80c6def4007e42dab39ef0fa92616302
Publikováno v:
Scientific Reports, Vol 8, Iss 1, Pp 1-15 (2018)
Abstract Small-molecule fluorescent probes are powerful and ubiquitous tools for measuring the concentration and distribution of analytes in living cells. However, accurate characterization of these analytes requires rigorous evaluation of cell-to-ce
Externí odkaz:
https://doaj.org/article/852635636b5e4591bccbb61e1037cb31
Publikováno v:
SSRN Electronic Journal.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ca2b3d462c9034686d86899392939c0d
https://doi.org/10.2139/ssrn.4318052
https://doi.org/10.2139/ssrn.4318052
Autor:
Shelby R. Lennon, Aleksandra J. Wierzba, Shea H. Siwik, Dorota Gryko, Amy E. Palmer, Robert T. Batey
RNA-targeting small molecule therapeutics an emerging field hindered by an incomplete understanding of the basic principles governing RNA-ligand interactions. One way to advance our knowledge in this area is to study model systems where these interac
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2b907f5cc5406c03770d8f42901bc7f9
https://doi.org/10.1101/2022.12.25.521693
https://doi.org/10.1101/2022.12.25.521693
Publikováno v:
PLoS Genetics, Vol 14, Iss 3, p e1007262 (2018)
Zinc is an essential trace element that is required for the function of a large number of proteins. As these zinc-binding proteins are found within the cytosol and organelles, all eukaryotes require mechanisms to ensure that zinc is delivered to orga
Externí odkaz:
https://doaj.org/article/c148a3582c6d4ec1af3cc57baaee115c