Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Amith D. Naik"'
Autor:
Tuhidul Islam, Yuki Ohara, Stefano Menegatti, Amith D. Naik, Takaaki Terasaka, Yasuhiro Hashimoto, Ruben G. Carbonell
Publikováno v:
Biochemical Engineering Journal. 145:53-61
A study is presented on the use of porous silica resins with tailored properties to develop affinity adsorbents for the purification of immunoglobulin G (IgG). Chromatorex® silica resins were utilized to study the dependence of IgG binding upon func
Autor:
Stefano Menegatti, William S. Kish, Ruben G. Carbonell, Matthew K. Roach, Hiroyuki Sachi, Amith D. Naik
Publikováno v:
Journal of Chromatography B. 1085:1-12
Prior work described the identification and characterization of erythropoietin-binding cyclic peptides SLFFLH, VVFFVH, FSLLHH and FSLLSH (all of the form cyclo[(Nα-Ac)Dap(A)-X1-X6-AE], wherein X1-X6 is the listed sequences). In this work, the peptid
Autor:
Matthew K. Roach, Hiroyuki Sachi, William S. Kish, Amith D. Naik, Stefano Menegatti, Benjamin G. Bobay, Ruben G. Carbonell, Robert Kevin Blackburn
Publikováno v:
Journal of Chromatography A. 1500:105-120
This work presents the selection and characterization of erythropoietin (EPO)-binding cyclic peptide ligands. The sequences were selected by screening a focused library of cyclic depsipeptides cyclo[(Nα-Ac)Dap(A)-X1-X6-AE], whose structure and amino
Autor:
Amith D. Naik, Benjamin G. Bobay, Kevin Lawrence Ward, William S. Kish, Tuhidul Islam, Stefano Menegatti, Ruben G. Carbonell
Publikováno v:
Journal of Chromatography A. 1445:93-104
A strategy is presented for developing variants of peptide ligands with enhanced biochemical stability for the purification of antibodies from animal sera. Antibody-binding sequences HWRGWV, HYFKFD, and HFRRHL, previously discovered by our group, wer
Publikováno v:
Food and Bioproducts Processing. 92:199-207
Protein A mimetic peptide ligands have several benefits over conventional Protein A/G ligands, namely that they are small in size, have low production costs, are stable over a wide range of pH values and can withstand cleaning by harsh sanitization a
Publikováno v:
International Journal of Molecular Sciences
Volume 20
Issue 1
Volume 20
Issue 1
This work presents the use of peptide ligand HWRGWV and its cognate sequences to develop affinity adsorbents that compete with Protein A in terms of binding capacity and quality of the eluted product. First, the peptide ligand was conjugated to cross
Publikováno v:
Pharmaceutical Bioprocessing. 1:467-485
While extensively used as drugs, small synthetic molecules have not yet been widely applied in the industry as affinity ligands for the purification of biopharmaceuticals. Yet, a substantial amount of published research indicates that synthetic ligan
Autor:
Kevin Lawrence Ward, Amith D. Naik, Ruben G. Carbonell, Robert Kevin Blackburn, William S. Kish, Stefano Menegatti
Publikováno v:
Analytical Chemistry. 85:9229-9237
A novel strategy is presented for the identification of cyclic peptide ligands from combinatorial libraries of reversible cyclic depsipeptides. A method for the solid-phase synthesis of individual cyclic depsipeptides and combinatorial libraries of t
Publikováno v:
Industrial & Engineering Chemistry Research. 52:8800-8811
High binding capacity and selectivity are key features for the successful application of affinity adsorbents for antibody purification. This study presents the development of affinity resins based on hexapeptide ligand HWRGWV for recovering monoclona
Publikováno v:
Journal of Chromatography A. 1260:61-66
The production of therapeutic proteins using transgenic plants offers several advantages, including low production cost, absence of human pathogens, presence of glycosylation mechanisms, and the ability to fold complex therapeutic proteins into their