Zobrazeno 1 - 10
of 15
pro vyhledávání: '"Amit T. Deshmukh"'
Autor:
Amit T. Deshmukh, Joseph J. Heijnen, Jianye Xia, Henk J. Noorman, Yingping Zhuang, Ju Chu, Cees Haringa, Siliang Zhang, Junfei Zhao, Guan Wang, Wenjun Tang, Walter M. van Gulik
Publikováno v:
Microbial Biotechnology, 11(3)
Microbial Biotechnology
Microbial Biotechnology
In a 54 m3 large-scale penicillin fermentor, the cells experience substrate gradient cycles at the timescales of global mixing time about 20–40 s. Here, we used an intermittent feeding regime (IFR) and a two-compartment reactor (TCR) to mimic these
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::96bb74b4eb6f162e9f9447b60fce52e2
https://doi.org/10.1111/1751-7915.13046
https://doi.org/10.1111/1751-7915.13046
Autor:
Wenjun Tang, Henk J. Noorman, Walter M. van Gulik, Cees Haringa, Robert F. Mudde, Guan Wang, Ju Chu, Amit T. Deshmukh, Joseph J. Heijnen, Wouter A. van Winden
Publikováno v:
Chemical Engineering Science, 175
We assess the effect of substrate heterogeneity on the metabolic response of P. chrysogenum in industrial bioreactors via the coupling of a 9-pool metabolic model with Euler-Lagrange CFD simulations. In this work, we outline how this coupled hydrodyn
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::59b40fd42bb84937119f10c3266d539c
https://doi.org/10.1016/j.ces.2017.09.020
https://doi.org/10.1016/j.ces.2017.09.020
Autor:
Jianye Xia, Walter M. van Gulik, Amit T. Deshmukh, Cees Haringa, Guan Wang, Matthias Reuss, Wenjun Tang, Wouter A. van Winden, Joseph J. Heijnen, Henk J. Noorman, Ju Chu
Publikováno v:
Biotechnology and Bioengineering. 114:1733-1743
A powerful approach for the optimization of industrial bioprocesses is to perform detailed simulations integrating large-scale computational fluid dynamics (CFD) and cellular reaction dynamics (CRD). However, complex metabolic kinetic models containi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a300f6464d7f0cc85f33a9aca9b674f1
https://doi.org/10.1002/bit.26294
https://doi.org/10.1002/bit.26294
Autor:
Joseph J. Heijnen, Cees Haringa, Jianye Xia, Henk J. Noorman, Amit T. Deshmukh, Wenjun Tang, Matthias Reuss, Robert F. Mudde
Publikováno v:
Engineering in Life Sciences
Engineering in Life Sciences, 16(7)
Engineering in Life Sciences, 16(7)
The trajectories, referred to as lifelines, of individual microorganisms in an industrial scale fermentor under substrate limiting conditions were studied using an Euler-Lagrange computational fluid dynamics approach. The metabolic response to substr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6d3b98f01b984ce3cbe59d98d20beed0
https://doi.org/10.1002/elsc.201600061
https://doi.org/10.1002/elsc.201600061
Autor:
Amit T. Deshmukh, Reza M. Seifar, Walter M. van Gulik, Joseph J. Heijnen, Peter J.T. Verheijen
Publikováno v:
Metabolic Engineering. 32:155-173
In this study we combined experimentation with mathematical modeling to unravel the in vivo kinetic properties of the enzymes and transporters of the penicillin biosynthesis pathway in a high yielding Penicillium chrysogenum strain. The experiment co
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4ced3ff8089455ff1e934f7dee1d6796
https://doi.org/10.1016/j.ymben.2015.09.018
https://doi.org/10.1016/j.ymben.2015.09.018
Autor:
Siliang Zhang, Guan Wang, Jianye Xia, Junfei Zhao, Walter M. van Gulik, Joseph J. Heijnen, Cees Haringa, Henk J. Noorman, Ju Chu, Yingping Zhuang, Amit T. Deshmukh, Baofeng Wu
Publikováno v:
Biotechnology and bioengineering. 115(1)
In the present work, by performing chemostat experiments at 400 and 600 RPM, two typical power inputs representative of industrial penicillin fermentation (P/V, 1.00 kW/m3 in more remote zones and 3.83 kW/m3 in the vicinity of the impellers, respecti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::64c1f536a7ac9a3fa3de0a3d21bb10e9
https://pubmed.ncbi.nlm.nih.gov/28865116
https://pubmed.ncbi.nlm.nih.gov/28865116
Publikováno v:
Chemical Engineering Science, 170
With reaction timescales equal to or shorter than the circulation time, the ideal mixing assumption typically does not hold for large scale bioreactors. As a consequence large scale gradients in extra-cellular conditions such as the substrate concent
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::377e44e85e2e151b9c749a3f2d9e0e68
Autor:
Camilo A. Suarez-Mendez, Joseph J. Heijnen, Ana Luisa B. Cruz, Reza M. Seifar, Cor Ras, Walter M. van Gulik, Katelijne M. Bekers, Amit T. Deshmukh
Publikováno v:
Journal of Chromatography A. 1311:115-120
A fast, sensitive and specific analytical method, based on ion pair reversed phase ultrahigh performance liquid chromatography tandem mass spectrometry, IP-RP-UHPLC-MS/MS, was developed for quantitative determination of intracellular coenzyme A (CoA)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::39eb4b8dd03f890f2377a8d789766d4f
https://doi.org/10.1016/j.chroma.2013.08.076
https://doi.org/10.1016/j.chroma.2013.08.076
Autor:
Wenjun, Tang, Amit T, Deshmukh, Cees, Haringa, Guan, Wang, Walter, van Gulik, Wouter, van Winden, Matthias, Reuss, Joseph J, Heijnen, Jianye, Xia, Ju, Chu, Henk J, Noorman
Publikováno v:
Biotechnology and bioengineering. 114(8)
A powerful approach for the optimization of industrial bioprocesses is to perform detailed simulations integrating large-scale computational fluid dynamics (CFD) and cellular reaction dynamics (CRD). However, complex metabolic kinetic models containi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::714c07c9008d4ca4f0134d82e904ac5a
https://pubmed.ncbi.nlm.nih.gov/28322433
https://pubmed.ncbi.nlm.nih.gov/28322433
Publikováno v:
Journal of Separation Science. 35:225-230
δ-[L-α-Aminoadipyl]-L-cysteinyl-D-valine (ACV) is a key intermediate in the biosynthesis pathway of penicillins and cephalosporins. Therefore, the accurate quantification of ACV is relevant, e.g. for kinetic studies on the production of these β-la
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::adae987cd9f25c0a55121ad73b704902
https://doi.org/10.1002/jssc.201100527
https://doi.org/10.1002/jssc.201100527