Systematic analysis of low-affinity transcription factor binding site clusters in vitro and in vivo establishes their functional relevance

Autor: Amir Shahein, Maria López-Malo, Ivan Istomin, Evan J. Olson, Shiyu Cheng, Sebastian J. Maerkl

Publikováno v: Nature Communications, Vol 13, Iss 1, Pp 1-17 (2022)

Here the authors quantitatively characterize binding to transcription factor (TF) binding site clusters in vitro, followed by characterizing clusters in synthetic and native gene regulatory systems in yeast. They show low-affinity clusters achieve hi

Externí odkaz: https://doaj.org/article/e8f7bcc128234561a5866a93d9992693

A workcell 1.0 for programmable and controlled operation of multiple fluidic chips in parallel

Autor: Chuanfang Ning, Gabriel Bunke, Simon Lietar, Lukas van den Heuvel, Amir Shahein

We developed a versatile lab-on-chip (LOC) workcell that enables the design and automatic execution of experiments on LOC devices, improving how we establish, optimize, and productionalize LOC processes. Key features include direct docking and coolin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::9db9af9bbaddc89f8c24cf4a2e2dd22a
https://doi.org/10.1101/2023.04.16.536594

Bottom-Up Construction of Complex Biomolecular Systems With Cell-Free Synthetic Biology

Autor: Grégoire Michielin, Sebastian J. Maerkl, Laura Grasemann, Zoe Swank, Amir Shahein, Barbora Lavickova, Nadanai Laohakunakorn

Publikováno v: Frontiers in Bioengineering and Biotechnology, Vol 8 (2020)
Laohakunakorn, N, Grasemann, L, Lavickova, B, Michielin, G, Shahein, A, Swank, Z & Maerkl, S J 2020, ' Bottom-up construction of complex biomolecular systems with cell-free synthetic biology ', Frontiers in Bioengineering and Biotechnology, vol. 8 . https://doi.org/10.3389/fbioe.2020.00213
Frontiers in Bioengineering and Biotechnology

Cell-free systems offer a promising approach to engineer biology since their open nature allows for well-controlled and characterized reaction conditions. In this review, we discuss the history and recent developments in engineering recombinant and c

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1fcdb4a4b443dae73f63e130aa768c93