Activation of subtilisin Carlsberg in organic solvents by methyl-β-cyclodextrin: Lyoprotection versus substrate and product-complex effect

Autor: Betzaida Castillo, Eduardo Rosario, Francesco Secundo, Amaris Ferrer, Angélica M. Santos, Elsa Silva, Ezio Fasoli, Gabriel Barletta, Kai Griebenow

Publikováno v: Journal of molecular catalysis. B, Enzymatic
42 (2006): 20–26. doi:10.1016/j.molcatb.2006.05.010
info:cnr-pdr/source/autori:SECUNDO, FRANCESCO (1); Fasoli, Ezio (2); Ferrer, Amaris (2); Barletta, Gabriel L. (2); Griebenow, Kai (2); Castillo, Betzaida (2); Silva, Elsa (2); Rosario, Eduardo (2); Santos, Angelica (2)/titolo:Activation of subtilisin Carlsberg in organic solvents by methyl-beta-cyclodextrin: Lyoprotection versus substrate and product-complex effect/doi:10.1016%2Fj.molcatb.2006.05.010/rivista:Journal of molecular catalysis. B, Enzymatic (Print)/anno:2006/pagina_da:20/pagina_a:26/intervallo_pagine:20–26/volume:42

Enzyme catalysis in organic solvents is a valuable tool for the synthesis of a number of important chiral compounds. However, their usually low activity in these applications is a major drawback which hampers their potential. To overcome this, resear

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b897a21f28ca9e9db0b9b9ac41b064d1
https://doi.org/10.1016/j.molcatb.2006.05.010

High Initial Activity but Low Storage Stability Observed for Several Preparations of Subtilisin Carslberg Suspended in Organic Solvents

Autor: Susimar González Martínez, Amaris Ferrer, Gabriel Barletta, Edgardo Alvira, Liz Vergara Cordero, Ileana Montañés‐Clemente

Publikováno v: Biotechnology Progress. 18:1462-1466

Colyophilization with methyl-beta-cyclodextrin activates subtilisin Carlsberg by more than 200-fold in organic solvents, though this is a short-lived effect. About 93% of the enzyme's high initial activity observed in THF (at 45 degrees C) decreases

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6e47d6ef9ec819f394e2344f124723ae
https://doi.org/10.1021/bp025650g

Photochemistry of water-soluble quinones. Production of the hydroxyl radical, singlet oxygen and the superoxide ion

Autor: Elsa Sepulveda, Antonio E. Alegria, Amaris Ferrer, Wilmarie Flores, Glysette Santiago

Publikováno v: Journal of Photochemistry and Photobiology A: Chemistry. 127:57-65

Quantum yields for the photosensitized production of singlet oxygen and superoxide ion by the several quinones 1,4-benzoquinone, BQ, 2-methyl-1,4-benzoquinone, MBQ, sodium 1,4-naphthoquinone-2-sulfonate, NQ2S, sodium 9,10-anthraquinone-2-sulfonate, A

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::dca4a11fb54b587b01c24d078caaeb87
https://doi.org/10.1016/s1010-6030(99)00138-0

Effect of PEG modification on subtilisin Carlsberg activity, enantioselectivity, and structural dynamics in 1,4-dioxane

Autor: Betzaida Castillo, Gabriel Barletta, Amaris Ferrer, Ricardo J. Solá, Kai Griebenow

Publikováno v: Biotechnology and bioengineering. 99(1)

The employment of enzymes as catalysts within organic media has traditionally been hampered by the reduced enzymatic activities when compared to catalysis in aqueous solution. Although several complementary hypotheses have provided mechanistic insigh

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d5c4ea46d3b742fd644d05ee43105055
https://pubmed.ncbi.nlm.nih.gov/17546684

Enzyme activation in organic solvents: co-lyophilization of subtilisin Carlsberg with methyl-beta-cyclodextrin renders an enzyme catalyst more active than the cross-linked enzyme crystals

Autor: Ileana, Montañez-Clemente, Edgardo, Alvira, Minedys, Macias, Amaris, Ferrer, Maricely, Fonceca, Jessica, Rodriguez, Anicele, Gonzalez, Gabriel, Barletta

Publikováno v: Biotechnology and bioengineering. 78(1)

In this study we explored the efficiency of the additive methyl-beta-cyclodextrin (M beta CD) to enhance the activity and enantioselectivity of the serine protease subtilisin Carlsberg in organic solvents. These two parameters, measured for different

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::360b8e30452d3fc65521bf3587030cc0
https://pubmed.ncbi.nlm.nih.gov/11857281