Zobrazeno 1 - 7
of 7
pro vyhledávání: '"Amanda H. Chan"'
Autor:
Aidan M. Fenix, Yuichiro Miyaoka, Alessandro Bertero, Steven M. Blue, Matthew J. Spindler, Kenneth K. B. Tan, Juan A. Perez-Bermejo, Amanda H. Chan, Steven J. Mayerl, Trieu D. Nguyen, Caitlin R. Russell, Paweena P. Lizarraga, Annie Truong, Po-Lin So, Aishwarya Kulkarni, Kashish Chetal, Shashank Sathe, Nathan J. Sniadecki, Gene W. Yeo, Charles E. Murry, Bruce R. Conklin, Nathan Salomonis
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-14 (2021)
Mutations in the splicing factor RBM20 cause aggressive Dilated Cardiomyopathy. Here the authors generated RBM20 R636S mutants and knockout in human iPSC-derived cardiomyocytes. Mutant RBM20 showed different target RNA binding, altered splicing and l
Externí odkaz:
https://doaj.org/article/f87c8729e80949bda7d8b7b816b64084
Autor:
Amanda H. Chan, Nathaniel Huebsch, Mohammad A. Mandegar, Annie Truong, Yuichiro Miyaoka, Ekaterina B. Frolov, Po-Lin So, Tilde Eskildsen, David E. Gordon, Lei S. Qi, Luke A. Gilbert, Kristin Holmes, Søren P. Sheikh, Bruce R. Conklin, Jacqueline E. Villalta, Edward Shin, Luke M. Judge, Jonathan S. Weissman, Nevan J. Krogan, Michael P. Olvera, Max A. Horlbeck, C. Ian Spencer
Publikováno v:
Cell stem cell, vol 18, iss 4
Mandegar, M A, Huebsch, N, Frolov, E B, Shin, E, Truong, A, Olvera, M P, Chan, A H, Miyaoka, Y, Holmes, K, Spencer, C I, Judge, L M, Gordon, D E, Eskildsen, T, Villalta, J E, Horlbeck, M A, Gilbert, L A, Krogan, N J, Sheikh, S P, Weissman, J S, Qi, L S, So, P-L & Conklin, B R 2016, ' CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs ', Cell Stem Cell, vol. 18, no. 4, pp. 541-553 . https://doi.org/10.1016/j.stem.2016.01.022
Mandegar, M A, Huebsch, N, Frolov, E B, Shin, E, Truong, A, Olvera, M P, Chan, A H, Miyaoka, Y, Holmes, K, Spencer, C I, Judge, L M, Gordon, D E, Eskildsen, T, Villalta, J E, Horlbeck, M A, Gilbert, L A, Krogan, N J, Sheikh, S P, Weissman, J S, Qi, L S, So, P-L & Conklin, B R 2016, ' CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs ', Cell Stem Cell, vol. 18, no. 4, pp. 541-553 . https://doi.org/10.1016/j.stem.2016.01.022
Developing technologies for efficient and scalable disruption of gene expression will provide powerful tools for studying gene function, developmental pathways, and disease mechanisms. Here, we develop clustered regularly interspaced short palindromi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::46dc91a30078b38e3cb997fe0327819a
https://doi.org/10.1016/j.stem.2016.01.022
https://doi.org/10.1016/j.stem.2016.01.022
Publikováno v:
Methods in Molecular Biology ISBN: 9781493977765
Genome editing holds great promise for experimental biology and potential clinical use. To successfully utilize genome editing, it is critical to sensitively detect and quantify its outcomes: homology-directed repair (HDR) and nonhomologous end joini
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9e992b5ba5344cc8fb32d0a977d374cc
https://pubmed.ncbi.nlm.nih.gov/29717453
https://pubmed.ncbi.nlm.nih.gov/29717453
Publikováno v:
Cold Spring Harbor protocols. 2016(8)
This protocol is designed to detect single-nucleotide substitutions generated by genome editing in a highly sensitive and quantitative manner. It uses a combination of allele-specific hydrolysis probes and a new digital polymerase chain reaction (dPC
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::16b438f7a29651ef616c0335bbd4723f
https://pubmed.ncbi.nlm.nih.gov/27250210
https://pubmed.ncbi.nlm.nih.gov/27250210
Publikováno v:
Cold Spring Harbor protocols. 2016(8)
The detection of genome editing is critical in evaluating genome-editing tools or conditions, but it is not an easy task to detect genome-editing events—especially single-nucleotide substitutions—without a surrogate marker. Here we introduce a pr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b39e148bb17ed9e7ea926cc9f53351f
https://pubmed.ncbi.nlm.nih.gov/27250209
https://pubmed.ncbi.nlm.nih.gov/27250209
Autor:
Yuichiro Miyaoka, Miller Huang, Bruce R. Conklin, Jennie Yoo, Amanda H. Chan, Paweena Lizarraga, Trieu Nguyen, Po-Lin So, Luke M. Judge
Publikováno v:
Nature methods
Miyaoka, Y; Chan, AH; Judge, LM; Yoo, J; Huang, M; Nguyen, TD; et al.(2014). Isolation of single-base genome-edited human iPS cells without antibiotic selection. Nature Methods, 11(3), 291-293. doi: 10.1038/nmeth.2840. UC San Francisco: Retrieved from: http://www.escholarship.org/uc/item/3bx0d4x4
Nature methods, vol 11, iss 3
Miyaoka, Y; Chan, AH; Judge, LM; Yoo, J; Huang, M; Nguyen, TD; et al.(2014). Isolation of single-base genome-edited human iPS cells without antibiotic selection. Nature Methods, 11(3), 291-293. doi: 10.1038/nmeth.2840. UC San Francisco: Retrieved from: http://www.escholarship.org/uc/item/3bx0d4x4
Nature methods, vol 11, iss 3
Precise editing of human genomes in pluripotent stem cells by homology-driven repair of targeted nuclease-induced cleavage has been hindered by the difficulty of isolating rare clones. We developed an efficient method to capture rare mutational event
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ba293ad186033834fc60b7fdf819aa21
http://europepmc.org/articles/PMC4063274
http://europepmc.org/articles/PMC4063274
Autor:
Amanda H. Chan, Yuichiro Miyaoka, Steven J. Mayerl, Jennifer R. Berman, Samantha Cooper, Bin Zhang, Bruce R. Conklin, George Karlin-Neumann
Publikováno v:
Scientific Reports
Scientific reports, vol 6, iss 1
Miyaoka, Y; Berman, JR; Cooper, SB; Mayerl, SJ; Chan, AH; Zhang, B; et al.(2016). Systematic quantification of HDR and NHEJ reveals effects of locus, nuclease, and cell type on genome-editing. SCIENTIFIC REPORTS, 6. doi: 10.1038/srep23549. UC San Francisco: Retrieved from: http://www.escholarship.org/uc/item/4f1594r9
Scientific reports, vol 6, iss 1
Miyaoka, Y; Berman, JR; Cooper, SB; Mayerl, SJ; Chan, AH; Zhang, B; et al.(2016). Systematic quantification of HDR and NHEJ reveals effects of locus, nuclease, and cell type on genome-editing. SCIENTIFIC REPORTS, 6. doi: 10.1038/srep23549. UC San Francisco: Retrieved from: http://www.escholarship.org/uc/item/4f1594r9
Precise genome-editing relies on the repair of sequence-specific nuclease-induced DNA nicking or double-strand breaks (DSBs) by homology-directed repair (HDR). However, nonhomologous end-joining (NHEJ), an error-prone repair, acts concurrently, reduc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a13b070f8f94105596b02266c8261555
https://pubmed.ncbi.nlm.nih.gov/27030102
https://pubmed.ncbi.nlm.nih.gov/27030102