Zobrazeno 1 - 10
of 16
pro vyhledávání: '"Amal Boctor"'
Autor:
Albert Grossman, Amal Boctor
Publikováno v:
Analytical Biochemistry. 86:769-775
After induction by cortisol, tyrosine aminotransferase ( l -tyrosine:2-oxoglutarate aminotransferase, EC 2.6.1.5) present in rat liver homogenates can be resolved into three peaks of activity by CM-Sephadex chromatography. Based on differential eluti
Publikováno v:
European Journal of Biochemistry. 44:391-400
Synthetic RNAs, i.e. [3H]poly(U) and [14C]poly(A), and natural RNAs, i.e. 16-S and 23-S Escherichia coli ribosomal [14C]RNA and MS2 coliphage [3H]RNA, were incompletely hydrolyzed by a rat liver nucleolar enzyme as measured by solubilization of radio
Publikováno v:
Journal of Biological Chemistry. 258:7284-7287
The cytosol fraction of rat pancrease can bind [3H] estradiol specifically and extensively. In contrast to the rat uterus, the binding protein in pancreas requires an accessory factor as a coligand in the steroid-binding reaction. Removal of this acc
Autor:
Amal Boctor, Albert Grossman
Publikováno v:
Proceedings of the National Academy of Sciences. 69:1161-1164
Induction of tyrosine aminotransferase (EC 2.6.1.5) with cortisol is followed by a rapid phase of inactivation of the enzyme, during which administration of puromycin causes a sharp increase in enzyme activity. The increase is not due to puromycin-re
Publikováno v:
Biochemical and Biophysical Research Communications. 36:721-727
Tyrosine aminotransferase (TA) was induced 5–8 fold by D-glucosamine (0.025M) in rat hepatoma cells grown in culture. This effect was observed only in a D-glucose deficient incubation medium. Under these conditions D-glucosamine cannot replace D-gl
Autor:
Amal Boctor, Albert Grossman
Publikováno v:
Biochemical Pharmacology. 22:17-28
α-Amanitin, an inhibitor of RNA polymerase derived from the mushroom Amanita phalloides , was shown to be about five times more potent than actinomycin-D in inhibiting rat liver tyrosine aminotransferase induction by cortisol. Rat hepatoma cells gro
Publikováno v:
Journal of steroid biochemistry. 19(2)
Following adrenalectomy of male rats, or adrenalectomy and ovariectomy of females, there was marked depletion of zymogen granules in acinar cells of the pancreas. Within 9 h after treatment with either triamcinolone or 17 beta-estradiol, complete res
Publikováno v:
Journal of receptor research. 2(5-6)
In parallel sets of experiments, cytosol fractions from rat pancreas and uterus were incubated with 2 nM 3H-estradiol in the presence or absence of nuclei from pancreas and liver. After incubation for 1 hr at room temperature, the nuclei were removed
Publikováno v:
Endocrinology. 113(2)
The cytosol fraction of rat pancreas [100,000 X g (for 1 h) supernatant] demonstrated specific but nonsaturable binding of [3H]estradiol in the concentration range of 2-50 X 10(-9) M. Scatchard analysis of specifically bound [3H]estradiol, determined
Publikováno v:
Journal of steroid biochemistry. 18(3)
The supernatant fraction of rat pancreas contains an estrogen-binding protein that differs in several ways from the one found in uterus: (1) binding of 17β-[ 3 H]-estradiol in pancreas was non-saturable at hormone concentrations ranging from 2–50