The Dimerization State of the Mammalian High Mobility Group Protein AT-Hook 2 (HMGA2).

Autor: Lorraine Frost, Maria A M Baez, Christopher Harrilal, Alyssa Garabedian, Francisco Fernandez-Lima, Fenfei Leng

Publikováno v: PLoS ONE, Vol 10, Iss 6, p e0130478 (2015)

The mammalian high mobility group protein AT-hook 2 (HMGA2) is a chromosomal architectural transcription factor involved in cell transformation and oncogenesis. It consists of three positively charged "AT-hooks" and a negatively charged C-terminus. S

Externí odkaz: https://doaj.org/article/8209ad5dfdc14a1081ba9a391d91adc4

Exploring the Conformations and Binding Location of HMGA2·DNA Complexes Using Ion Mobility Spectrometry and 193 nm Ultraviolet Photodissociation Mass Spectrometry

Autor: Sarah N. Sipe, Kevin Jeanne Dit Fouque, Alyssa Garabedian, Fenfei Leng, Francisco Fernandez-Lima, Jennifer S. Brodbelt

Publikováno v: J Am Soc Mass Spectrom

Although it is widely accepted that protein function is largely dependent on its structure, intrinsically disordered proteins (IDPs) lack defined structure but are essential in proper cellular processes. Mammalian high mobility group proteins (HMGA)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e61f27b7852110977f1db169fa3e181a
https://doi.org/10.1021/jasms.2c00083

Identification of HMGA2 inhibitors by AlphaScreen-based ultra-high-throughput screening assays

Autor: Stefan Vasile, Fenfei Leng, Sabrina Battista, Juliano Freitas, Jeremy W. Chambers, Alyssa Garabedian, Federica D'Alessio, Miriam Romano, Prem P. Chapagain, Linjia Su, Francisco Fernandez-Lima, Alfredo Fusco, Layton H. Smith, Nadezda Bryan, Fabiana Falanga, Lidia Kos

Publikováno v: Scientific Reports, Vol 10, Iss 1, Pp 1-14 (2020)
Scientific Reports

The mammalian high mobility group protein AT-hook 2 (HMGA2) is a multi-functional DNA-binding protein that plays important roles in tumorigenesis and adipogenesis. Previous results showed that HMGA2 is a potential therapeutic target of anticancer and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5365454f43ac3a8bd46721c3dc7d0189
http://link.springer.com/article/10.1038/s41598-020-75890-0

Peptide Sequence Influence on the Conformational Dynamics and DNA binding of the Intrinsically Disordered AT-Hook 3 Peptide

Autor: Francisco Fernandez-Lima, Fenfei Leng, Alexander Bolufer, Alyssa Garabedian

Publikováno v: Scientific Reports, Vol 8, Iss 1, Pp 1-8 (2018)
Scientific Reports

The intrinsically disordered ATHP3 was studied at native conditions and in complex with DNA using single amino acid substitutions and high-resolution ion mobility spectrometry coupled to mass spectrometry (trapped IMS-MS). Results showed that ATHP3 c

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f707a261122be5233aa076ca2b933647
http://link.springer.com/article/10.1038/s41598-018-28956-z

Linear and Differential Ion Mobility Separations of Middle-Down Proteoforms

Autor: Ole N. Jensen, Todd D. Williams, Kevin Jeanne Dit Fouque, Alexandre A. Shvartsburg, Matthew A. Baird, Alyssa Garabedian, Jacob Porter, Pavel V. Shliaha, Francisco Fernandez-Lima

Publikováno v: Garabedian, A, Baird, M A, Porter, J, Jeanne Dit Fouque, K, Shliaha, P V, Jensen, O N, Williams, T D, Fernandez-Lima, F & Shvartsburg, A A 2018, ' Linear and Differential Ion Mobility Separations of Middle-Down Proteoforms ', Analytical Chemistry, vol. 90, no. 4, pp. 2918-2925 . https://doi.org/10.1021/acs.analchem.7b05224

Comprehensive characterization of proteomes comprising the same proteins with distinct post-translational modifications (PTMs) is a staggering challenge. Many such proteoforms are isomers (localization variants) that require separation followed by to

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d07f9942068e080cfdc95fba5397e072
https://doi.org/10.1021/acs.analchem.7b05224