Zobrazeno 1 - 10
of 18
pro vyhledávání: '"Altea Gosálbez"'
Autor:
Jaime Gosálvez, Stephen Johnston, Carmen López-Fernández, Altea Gosálbez, Francisca Arroyo, Jose Luís Fernández, Juan GÁlvarez
Publikováno v:
Asian Pacific Journal of Reproduction, Vol 3, Iss 2, Pp 116-120 (2014)
Objective: To investigate the DNA longevity characteristics associated with each resultant fraction following density gradient centrifugation (DGC) in comparison to that of the original neat ejaculated sample. Methods: An aliquot of neat semen (NSS)
Externí odkaz:
https://doaj.org/article/2078b0fa3671447b9d0dc6b7e8ee9786
Autor:
Stephen D. Johnston, José Luís Fernández, Jaime Gosálvez, F. Arroyo, Carmen López-Fernández, Altea Gosálbez, Elva I. Cortés Gutiérrez
Publikováno v:
Journal of Assisted Reproduction and Genetics. 33:1231-1238
Using a rabbit model, we assessed the influence of sperm DNA longevity on female reproductive outcomes. Semen was collected from 40 bucks, incubated at 38 °C for 24 h, and the rate of sperm DNA fragmentation (rSDF) was determined using the sperm chr
Autor:
Carmen López-Fernández, Altea Gosálbez, F. Arroyo, Stephen D. Johnston, José Luís Fernández, Juan GÁlvarez, Jaime Gosálvez
Publikováno v:
Asian Pacific Journal of Reproduction, Vol 3, Iss 2, Pp 116-120 (2014)
Objective To investigate the DNA longevity characteristics associated with each resultant fraction following density gradient centrifugation (DGC) in comparison to that of the original neat ejaculated sample. Methods An aliquot of neat semen (NSS) co
Autor:
Jaime Gosálvez, Carmen López-Fernández, Altea Gosálbez, José Luis Fernández, Juan G. Alvarez, Stephen D. Johnston
Publikováno v:
Natural Science. :15-20
This was a prospective multicenter study aiming at comparing the efficiency of sperm selection by density gradient centrifugation (DGC) in reducing sperm DNA fragmentation (SDF) in different ART centers. The study was designed using 290 semen samples
Publikováno v:
Small Ruminant Research. 100:50-53
Evidence is provided of seasonal changes in the rate of DNA fragmentation dynamics of sperm collected from goats Murciano-Granadina breed), in a Spanish Breeding Centre at 41°N latitude. Results show that the initial assessment of sperm DNA fragment
Autor:
Martha I. Dávila-Rodríguez, Carmen López-Fernández, Altea Gosálbez, Elva I. Cortés-Gutiérrez, Jaime Gosálvez, José L. Fernández
Publikováno v:
Journal of Histochemistry & Cytochemistry. 59:655-660
The comet assay is a well-established, simple, versatile, visual, rapid, and sensitive tool used extensively to assess DNA damage and DNA repair quantitatively and qualitatively in single cells. The comet assay is most frequently used to analyze whit
Autor:
F. Arroyo, Matthew J. G. Gage, Jaime Gosálvez, Ana M Larrán, Altea Gosálbez, José Luis Fernández, Carmen López-Fernández
Publikováno v:
REPRODUCTION. 138:257-266
Spermatozoal haplotypic DNA is prone to damage, leading to male fertility problems. So far, the assessment of sperm DNA breakage has been challenging because protamines render the nuclear chromatin highly compacted. Here, we report the application of
Autor:
Altea Gosálbez, William V. Holt, F. Arroyo, Carmen López-Fernández, Jaime Gosálvez, José L. Fernández, Juan M. Vazquez
Publikováno v:
Theriogenology. 70:898-908
From a biological viewpoint spermatozoa are ejaculated by the male and received into the female while maintaining roughly constant temperature, which in most mammals is below the temperature of the soma. When ejaculated spermatozoa are used for artif
Autor:
M. Enciso, Juan M. Vazquez, Altea Gosálbez, Jon R. Bridle, C. López-Fernández, Jaime Gosálvez, José L. Fernández
Publikováno v:
The Open Veterinary Science Journal. 2:7-10
Sperm DNA fragmentation was analysed in 113 semen samples obtained from different rams over a period of one year. Semen samples were collected from: unvaccinated rams between January and June (Control group 1; CG1); vaccinated rams at least 70 days a
Publikováno v:
Biotechnic & Histochemistry. 83:247-252
The sperm chromatin dispersion (SCD) test is a new technique that allows assessment of sperm DNA fragmentation (SDF) in different species. The application of this technique, like other techniques, is restricted to the laboratory. Our investigation wa