Reconstitution of actin-based cellular processes: Why encapsulation changes the rules

Autor: Fabina Binth Kandiyoth, Alphée Michelot

Publikováno v: European Journal of Cell Biology, Vol 102, Iss 4, Pp 151368- (2023)

While in vitro reconstitution of cellular processes is progressing rapidly, the encapsulation of biomimetic systems to reproduce the cellular environment is a major challenge. Here we review the difficulties, using reconstitution of processes depende

Externí odkaz: https://doaj.org/article/640ad7cd117c4b289cfdc13dae0413d7

Diversity from similarity: cellular strategies for assigning particular identities to actin filaments and networks

Autor: Micaela Boiero Sanders, Adrien Antkowiak, Alphée Michelot

Publikováno v: Open Biology, Vol 10, Iss 9 (2020)

The actin cytoskeleton has the particularity of being assembled into many functionally distinct filamentous networks from a common reservoir of monomeric actin. Each of these networks has its own geometrical, dynamical and mechanical properties, beca

Externí odkaz: https://doaj.org/article/ceb2cdcd0ffa4eaebd3f872ce4bcedf8

Sizes of actin networks sharing a common environment are determined by the relative rates of assembly.

Autor: Adrien Antkowiak, Audrey Guillotin, Micaela Boiero Sanders, Jessica Colombo, Renaud Vincentelli, Alphée Michelot

Publikováno v: PLoS Biology, Vol 17, Iss 6, p e3000317 (2019)

Within the cytoplasm of a single cell, several actin networks can coexist with distinct sizes, geometries, and protein compositions. These actin networks assemble in competition for a limited pool of proteins present in a common cellular environment.

Externí odkaz: https://doaj.org/article/3e8c0f68281146e9a4aa53711f72ba4a

A functional family of fluorescent nucleotide analogues to investigate actin dynamics and energetics

Autor: Jenna Elliott, Jessica Colombo, Pekka Lappalainen, Konstantin Kogan, Alphée Michelot, Tommi Kotila, Audrey Guillotin, Adrien Antkowiak

Publikováno v: Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
Nature Communications
Nature Communications, Nature Publishing Group, 2021, 12 (1), ⟨10.1038/s41467-020-20827-4⟩
Nature Communications, Nature Publishing Group, 2021, 12, pp.548. ⟨10.1038/s41467-020-20827-4⟩
Nature Communications, 2021, 12 (1), ⟨10.1038/s41467-020-20827-4⟩
Nature Communications, 2021, 12, pp.548. ⟨10.1038/s41467-020-20827-4⟩

Actin polymerization provides force for vital processes of the eukaryotic cell, but our understanding of actin dynamics and energetics remains limited due to the lack of high-quality probes. Most current probes affect dynamics of actin or its interac

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c6dc536806899363eef32858a0c0e224
https://doaj.org/article/887eda43f8084479b43243044a31eee3

Membrane-Sculpting BAR Domains Generate Stable Lipid Microdomains

Autor: Hongxia Zhao, Alphée Michelot, Essi V. Koskela, Vadym Tkach, Dimitrios Stamou, David G. Drubin, Pekka Lappalainen

Publikováno v: Cell Reports, Vol 4, Iss 6, Pp 1213-1223 (2013)

Bin-Amphiphysin-Rvs (BAR) domain proteins are central regulators of many cellular processes involving membrane dynamics. BAR domains sculpt phosphoinositide-rich membranes to generate membrane protrusions or invaginations. Here, we report that, in ad

Externí odkaz: https://doaj.org/article/661a76fa376d494296191a54bdf699ec