Evolving dual-trait EPSP synthase variants using a synthetic yeast selection system.

Autor: Reed KB; McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX 78712., Kim W; McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX 78712., Lu H; McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX 78712., Larue CT; Crop Science Division, Bayer, Chesterfield, MO 63017., Guo S; Crop Science Division, Bayer, Chesterfield, MO 63017., Brooks SM; McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX 78712., Montez MR; McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX 78712., Wagner JM; McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX 78712., Zhang YJ; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX 78712., Alper HS; McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX 78712.; Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX 78712.

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2024 Aug 27; Vol. 121 (35), pp. e2317027121. Date of Electronic Publication: 2024 Aug 19.

Author Correction: A modular and synthetic biosynthesis platform for de novo production of diverse halogenated tryptophan-derived molecules.

Autor: Reed KB; McKetta Department of Chemical Engineering, The University of Texas at Austin, 200 E Dean Keeton St. Stop C0400, Austin, TX, USA., Brooks SM; McKetta Department of Chemical Engineering, The University of Texas at Austin, 200 E Dean Keeton St. Stop C0400, Austin, TX, USA., Wells J; McKetta Department of Chemical Engineering, The University of Texas at Austin, 200 E Dean Keeton St. Stop C0400, Austin, TX, USA., Blake KJ; Mass Spectrometry Facility, Department of Chemistry, The University of Texas at Austin, 105 E 24th Street, Austin, TX, USA., Zhao M; McKetta Department of Chemical Engineering, The University of Texas at Austin, 200 E Dean Keeton St. Stop C0400, Austin, TX, USA., Placido K; McKetta Department of Chemical Engineering, The University of Texas at Austin, 200 E Dean Keeton St. Stop C0400, Austin, TX, USA., d'Oelsnitz S; Institute for Cellular and Molecular Biology, The University of Texas at Austin, 2500 Speedway Avenue, Austin, TX, USA., Trivedi A; McKetta Department of Chemical Engineering, The University of Texas at Austin, 200 E Dean Keeton St. Stop C0400, Austin, TX, USA., Gadhiyar S; McKetta Department of Chemical Engineering, The University of Texas at Austin, 200 E Dean Keeton St. Stop C0400, Austin, TX, USA., Alper HS; McKetta Department of Chemical Engineering, The University of Texas at Austin, 200 E Dean Keeton St. Stop C0400, Austin, TX, USA. halper@che.utexas.edu.; Institute for Cellular and Molecular Biology, The University of Texas at Austin, 2500 Speedway Avenue, Austin, TX, USA. halper@che.utexas.edu.

Publikováno v: Nature communications [Nat Commun] 2024 Jun 07; Vol. 15 (1), pp. 4868. Date of Electronic Publication: 2024 Jun 07.

Leveraging a Y. lipolytica naringenin chassis for biosynthesis of apigenin and associated glucoside.

Autor: Marsan CB; McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, 78712, USA., Lee SG; McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, 78712, USA., Nguyen A; McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, 78712, USA., Gordillo Sierra AR; McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, 78712, USA., Coleman SM; McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, 78712, USA., Brooks SM; McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, 78712, USA., Alper HS; McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, 78712, USA; Interdisciplinary Life Sciences Program, The University of Texas at Austin, Austin, TX, 78712, USA. Electronic address: halper@che.utexas.edu.

Publikováno v: Metabolic engineering [Metab Eng] 2024 May; Vol. 83, pp. 1-11. Date of Electronic Publication: 2024 Mar 04.

Fluorescence-Based Screens for Engineering Enzymes Linked to Halogenated Tryptophan.

Autor: Reed KB; McKetta Department of Chemical Engineering, The University of Texas at Austin, 200 E. Dean Keeton Street Stop C0400, Austin, Texas 78712, United States., d'Oelsnitz S; Institute for Cellular and Molecular Biology, The University of Texas at Austin, 2500 Speedway Avenue, Austin, Texas 78712, United States., Brooks SM, Wells J; McKetta Department of Chemical Engineering, The University of Texas at Austin, 200 E. Dean Keeton Street Stop C0400, Austin, Texas 78712, United States., Zhao M; McKetta Department of Chemical Engineering, The University of Texas at Austin, 200 E. Dean Keeton Street Stop C0400, Austin, Texas 78712, United States., Trivedi A; McKetta Department of Chemical Engineering, The University of Texas at Austin, 200 E. Dean Keeton Street Stop C0400, Austin, Texas 78712, United States., Eshraghi S; McKetta Department of Chemical Engineering, The University of Texas at Austin, 200 E. Dean Keeton Street Stop C0400, Austin, Texas 78712, United States., Alper HS; McKetta Department of Chemical Engineering, The University of Texas at Austin, 200 E. Dean Keeton Street Stop C0400, Austin, Texas 78712, United States.; Institute for Cellular and Molecular Biology, The University of Texas at Austin, 2500 Speedway Avenue, Austin, Texas 78712, United States.

Publikováno v: ACS synthetic biology [ACS Synth Biol] 2024 Apr 19; Vol. 13 (4), pp. 1373-1381. Date of Electronic Publication: 2024 Mar 27.

Design and synthesis of synthetic promoters for consistency of gene expression across growth phases and scale in S. cerevisiae .

Autor: Presnell KV; McKetta Department of Chemical Engineering, The University of Texas at Austin, 200E Dean Keeton St. Stop C0400, Austin, TX, 78712, USA., Melhem O; McKetta Department of Chemical Engineering, The University of Texas at Austin, 200E Dean Keeton St. Stop C0400, Austin, TX, 78712, USA., Coleman SM; McKetta Department of Chemical Engineering, The University of Texas at Austin, 200E Dean Keeton St. Stop C0400, Austin, TX, 78712, USA., Morse NJ; McKetta Department of Chemical Engineering, The University of Texas at Austin, 200E Dean Keeton St. Stop C0400, Austin, TX, 78712, USA., Alper HS; McKetta Department of Chemical Engineering, The University of Texas at Austin, 200E Dean Keeton St. Stop C0400, Austin, TX, 78712, USA.; Institute for Cellular and Molecular Biology, The University of Texas at Austin, 2500 Speedway Avenue, Austin, TX, 78712, USA.

Publikováno v: Synthetic and systems biotechnology [Synth Syst Biotechnol] 2024 Mar 13; Vol. 9 (2), pp. 330-339. Date of Electronic Publication: 2024 Mar 13 (Print Publication: 2024).