Zobrazeno 1 - 10
of 88
pro vyhledávání: '"Alon Greenbaum"'
Autor:
Amanda B. T. Hill, Yanet M. Murphy, Kathryn M. Polkoff, Laura Edwards, Derek M. Walker, Adele Moatti, Alon Greenbaum, Jorge A. Piedrahita
Publikováno v:
Frontiers in Genome Editing, Vol 6 (2024)
Recent advancements in genome editing techniques, notably CRISPR-Cas9 and TALENs, have marked a transformative era in biomedical research, significantly enhancing our understanding of disease mechanisms and helping develop novel therapies. These tech
Externí odkaz:
https://doaj.org/article/5275e6c26dd74e15a4a927b31165176a
Publikováno v:
Intelligent Computing, Vol 3 (2024)
Light-sheet fluorescence microscopy (LSFM) provides the benefit of optical sectioning coupled with rapid acquisition times, enabling high-resolution 3-dimensional imaging of large tissue-cleared samples. Inherent to LSFM, the quality of the imaging h
Externí odkaz:
https://doaj.org/article/e48583b368c54e84bd1cda167ecb4b4e
Autor:
Adele Moatti, Shannon Connard, Novietta De Britto, William A. Dunn, Srishti Rastogi, Mani Rai, Lauren V. Schnabel, Frances S. Ligler, Kendall A. Hutson, Douglas C. Fitzpatrick, Alec Salt, Carlton J. Zdanski, Alon Greenbaum
Publikováno v:
Frontiers in Pharmacology, Vol 15 (2024)
Introduction: One major obstacle in validating drugs for the treatment or prevention of hearing loss is the limited data available on the distribution and concentration of drugs in the human inner ear. Although small animal models offer some insights
Externí odkaz:
https://doaj.org/article/fcd4db90412f4a2181ab6dd7284bc67e
Autor:
Adele Moatti, Dylan Silkstone, Taylor Martin, Keith Abbey, Kendall A Hutson, Douglas C Fitzpatrick, Carlton J Zdanski, Alan G Cheng, Frances S Ligler, Alon Greenbaum
Publikováno v:
iScience, Vol 26, Iss 6, Pp 106789- (2023)
Summary: Delivery of pharmaceutical therapeutics to the inner ear to treat and prevent hearing loss is challenging. Systemic delivery is not effective as only a small fraction of the therapeutic agent reaches the inner ear. Invasive surgeries to inje
Externí odkaz:
https://doaj.org/article/9b43e426dc0e496bb366311746e7fbe3
Autor:
Adele Moatti, Yuheng Cai, Chen Li, Kristen D. Popowski, Ke Cheng, Frances S. Ligler, Alon Greenbaum
Publikováno v:
STAR Protocols, Vol 4, Iss 2, Pp 102220- (2023)
Summary: The inner ear of humans and large animals is embedded in a thick and dense bone that makes dissection challenging. Here, we present a protocol that enables three-dimensional (3D) characterization of intact inner ears from large-animal models
Externí odkaz:
https://doaj.org/article/613d1cb6b9d9451aa2dfe8560a1658a7
Autor:
Xuying Zhang, Guanxi Xiao, Caroline Johnson, Yuheng Cai, Zachary K. Horowitz, Christine Mennicke, Robert Coffey, Mansoor Haider, David Threadgill, Rebecca Eliscu, Michael C. Oldham, Alon Greenbaum, H. Troy Ghashghaei
Publikováno v:
iScience, Vol 26, Iss 3, Pp 106242- (2023)
Summary: The epidermal growth factor receptor (EGFR) plays a role in cell proliferation and differentiation during healthy development and tumor growth; however, its requirement for brain development remains unclear. Here we used a conditional mouse
Externí odkaz:
https://doaj.org/article/009e161e25ab4ba485fffac48cf84c13
Autor:
Adele Moatti, Chen Li, Sasank Sivadanam, Yuheng Cai, James Ranta, Jorge A. Piedrahita, Alan G. Cheng, Frances S. Ligler, Alon Greenbaum
Publikováno v:
iScience, Vol 25, Iss 8, Pp 104695- (2022)
Summary: Over 11% of the world’s population experience hearing loss. Although there are promising studies to restore hearing in rodent models, the size, ontogeny, genetics, and frequency range of hearing of most rodents’ cochlea do not match that
Externí odkaz:
https://doaj.org/article/3f08679200f540b29ba454ca1c49df1f
Autor:
Anat Kahan, Alon Greenbaum, Min J. Jang, J. Elliott Robinson, Jounhong Ryan Cho, Xinhong Chen, Pegah Kassraian, Daniel A. Wagenaar, Viviana Gradinaru
Publikováno v:
Cell Reports, Vol 36, Iss 13, Pp 109744- (2021)
Summary: Optical implants to control and monitor neuronal activity in vivo have become foundational tools of neuroscience. Standard two-dimensional histology of the implant location, however, often suffers from distortion and loss during tissue proce
Externí odkaz:
https://doaj.org/article/f3eba9be561744f1af07ea6e1dad34ec
Autor:
Pradeep S. Rajendran, Rosemary C. Challis, Charless C. Fowlkes, Peter Hanna, John D. Tompkins, Maria C. Jordan, Sarah Hiyari, Beth A. Gabris-Weber, Alon Greenbaum, Ken Y. Chan, Benjamin E. Deverman, Heike Münzberg, Jeffrey L. Ardell, Guy Salama, Viviana Gradinaru, Kalyanam Shivkumar
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
The wiring of peripheral neural circuits that regulate heart rate is poorly understood. In this study, authors used tissue clearing for high-resolution characterization of nerves in the heart in 3D and transgenic and novel viral vector approaches to
Externí odkaz:
https://doaj.org/article/e62e7c209ae04211ac168bacbbdd12fb
Publikováno v:
PLoS ONE, Vol 16, Iss 9, p e0257426 (2021)
The ability to automatically detect and classify populations of cells in tissue sections is paramount in a wide variety of applications ranging from developmental biology to pathology. Although deep learning algorithms are widely applied to microscop
Externí odkaz:
https://doaj.org/article/8d8d4ca159c041cfaf050e1cf75338e6