Výsledky vyhledávání - "Allyson Masci"

Akademický článek

Macrophage-specific chemokines induced via innate immunity by amino acid copolymers and their role in EAE.

Autor: Joseph Kovalchin, Jeffrey Krieger, Michelle Genova, Norio Kawamoto, Michael Augustyniak, Kathryn Collins, Troy Bloom, Allyson Masci, Tara Hittinger, Ingrid Dufour, Jack L Strominger, Eric Zanelli

Publikováno v: PLoS ONE, Vol 6, Iss 12, p e26274 (2011)

The random amino acid copolymer poly(Y,E,A,K)(n) (Copaxone®) is widely used in multiple sclerosis treatment and a second generation copolymer poly(Y,F,A,K)(n) with enhanced efficacy in experimental autoimmune encephalomyelitis in mice has been descr

Externí odkaz: https://doaj.org/article/f4cfbffef4684307ab08efd695f6a2ba

Zobrazit plný text záznamu

Integration of Fluorescence Detection and Image-Based Automated Counting Increases Speed, Sensitivity, and Robustness of Plaque Assays

Autor: Wei-Chiang Chen, Carl Co, Xiaohui Lu, Emily Menesale, Svetlana Bergelson, Allyson Masci

Publikováno v: Molecular Therapy. Methods & Clinical Development

Plaque assays are used to measure the infectious titer of viral samples. These assays are multi-day and low-throughput and may be subject to analyst variability from biased or subjective manual plaque counting. Typically, on day 1, cells are adhered

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c3f46cc5b38fea0cbe8368470bdb8c36
https://doi.org/10.1016/j.omtm.2019.07.007

Zobrazit plný text záznamu

Biolayer Interferometry-based FcγRIIa binding assay for a therapeutic antibody with strong effector function

Autor: Adriana Bajardi-Taccioli, Tim Brantley, Marina Feschenko, Christine Bond, Allyson Masci, Chongfeng Xu, Svetlana Bergelson, Carl Co

Publikováno v: Analytical biochemistry. 611

FcγRIIa receptor binding is part of the mechanism of action for many therapeutic antibodies. AlphaScreen® technology and Biolayer Interferometry (BLI) are often used to assess protein-protein interactions. Recently we demonstrated that the presence

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::95eed7a6ffabf7ce4cd4e9c467710fd7
https://pubmed.ncbi.nlm.nih.gov/32755599

Zobrazit plný text záznamu

Preclinical Development of EBI-005: An IL-1 Receptor-1 Inhibitor for the Topical Ocular Treatment of Ocular Surface Inflammatory Diseases

Autor: Steve Weber, Kelly Tenneson, Eric Furfine, Gary Wolfe, Elizabeth Hopkins, Jay Hou, Joseph Kovalchin, Allyson Masci, Christian Li, Kathy Collins, Bracken M. King, Jeremy Fry

Publikováno v: Eyecontact lens. 44(3)

Objective Topical interleukin (IL)-1 receptor (R)1 blockade is therapeutically active in reducing signs and symptoms of dry eye disease. Herein, we describe in vitro and in vivo nonclinical Investigational New Drug (IND)-enabling studies of EBI-005,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c7bfb22275cbdbb4aaa6ebe898b11224
https://pubmed.ncbi.nlm.nih.gov/28727604

Zobrazit plný text záznamu

Design of a superior cytokine antagonist for topical ophthalmic use

Autor: Gregory Zarbis-Papastoitsis, Allyson Masci, Christoph Thomas, Sharon A. Townson, Olga Kiner, Kathryn Golden, Jinzhao Hou, Joseph Kovalchin, Yanqun Shu, Emily Belcher Schirmer, Eric Furfine, K. Christopher Garcia, Thomas M. Barnes, Bracken M. King

Publikováno v: Proceedings of the National Academy of Sciences. 110:3913-3918

IL-1 is a key inflammatory and immune mediator in many diseases, including dry-eye disease, and its inhibition is clinically efficacious in rheumatoid arthritis and cryopyrin-associated periodic syndromes. To treat ocular surface disease with a topic

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a402ff48f7d311efbbe60a2d03ab1268
https://doi.org/10.1073/pnas.1217996110

Zobrazit plný text záznamu

Safety, Pharmacokinetic, and Pharmacodynamic Evaluations of PI-2301, a Potent Immunomodulator, in a First-in-Human, Single-Ascending-Dose Study in Healthy Volunteers

Autor: Edward Mascioli, Nicolas Fauchoux, Jeffrey Krieger, Michelle Genova, E. Lacoste, Joseph Kovalchin, Michael Augustyniak, Kathy Collins, Tony Avril, Claire Toutin, Uday Patel, Eric Zanelli, Alain Patat, Gwenola Gandon, Allyson Masci

Publikováno v: The Journal of Clinical Pharmacology. 51:649-660

PI-2301 is an amino acid copolymer acting as an immunomodulator for the treatment of autoimmune diseases. The present study evaluated the safety, pharmacokinetics (PK), and pharmacodynamics of PI-2301 in a single ascending dose, first-in-human study

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6203d2e89a9eb5af40e65ec24b172f29
https://doi.org/10.1177/0091270010373930

Zobrazit plný text záznamu

A simple enzyme-substrate localized conjugation method to generate immobilized, functional glutathione S-transferase fusion protein columns for affinity enrichment

Autor: Svetlana Bergelson, Carl Co, Robert S. Gronke, John Coughlin, Allyson Masci

Publikováno v: Analytical biochemistry. 505

Immobilized protein receptors and enzymes are tools for isolating or enriching ligands and substrates based on affinity. For example, glutathione S-transferase (GST) is fused to proteins as a tag for binding to its substrate glutathione (GSH) linked

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7009a7b17e74f315e530d199f35b3633
https://pubmed.ncbi.nlm.nih.gov/27063248

Zobrazit plný text záznamu

Crystal structure of an HSA/FcRn complex reveals recycling by competitive mimicry of HSA ligands at a pH-dependent hydrophobic interface

Autor: Alison Tisdale, Joseph Kovalchin, Allyson Masci, Alec J. Murillo, David V. Erbe, Patricia Lowden, Michael M. Schmidt, Sharon A. Townson, K. Dane Wittrup, Emily Belcher Schirmer, Eric Furfine, Christian Dombrowski, Thomas M. Barnes, Bracken M. King, Amy Jada Andreucci

Publikováno v: Structure (London, England : 1993). 21(11)

SummaryThe long circulating half-life of serum albumin, the most abundant protein in mammalian plasma, derives from pH-dependent endosomal salvage from degradation, mediated by the neonatal Fc receptor (FcRn). Using yeast display, we identified human

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8785f7b23faee980ba2a545f5c3432b3
https://pubmed.ncbi.nlm.nih.gov/24210225

Zobrazit plný text záznamu

Reduction of product-related species during the fermentation and purification of a recombinant IL-1 receptor antagonist at the laboratory and pilot scale

Autor: Gregory Zarbis-Papastoitsis, Emily Belcher Schirmer, Alec J. Murillo, Jinzhao Hou, Allyson Masci, Kathryn Collins, Kathryn Golden, Jin Xu, Joseph Kovalchin, Sri Hari Raju Mulagapati, Patricia Lowden, Jesse Milling

Publikováno v: Biotechnology journal. 8(8)

Through a parallel approach of tracking product quality through fermentation and purification development, a robust process was designed to reduce the levels of product-related species. Three biochemically similar product-related species were identif

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::03c4a4aff7adfade469a25a4ce58f924
https://pubmed.ncbi.nlm.nih.gov/23913906

Zobrazit plný text záznamu

Macrophage-Specific Chemokines Induced via Innate Immunity by Amino Acid Copolymers and Their Role in EAE

Autor: Jeffrey Krieger, Ingrid Dufour, Tara Hittinger, Norio Kawamoto, Jack L. Strominger, Eric Zanelli, Michelle Genova, Allyson Masci, Kathryn Collins, Michael Augustyniak, Troy Bloom, Joseph Kovalchin

Publikováno v: PLoS ONE, Vol 6, Iss 12, p e26274 (2011)
PLoS ONE

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a9242a2d894b0c4517d2d893db43cd3
https://doi.org/10.1371/journal.pone.0026274

Zobrazit plný text záznamu

Vyhledávací nástroje:

Upřesnit hledání