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Autor:
Schubert, Claudia1 (AUTHOR), Allhoff, Manuel2 (AUTHOR), Tillmann, Stefan1 (AUTHOR), Maié, Tiago2 (AUTHOR), Costa, Ivan G.2 (AUTHOR), Lipka, Daniel B.3 (AUTHOR), Schemionek, Mirle1 (AUTHOR), Feldberg, Kristina1 (AUTHOR), Baumeister, Julian1 (AUTHOR), Brümmendorf, Tim H.1 (AUTHOR), Chatain, Nicolas1 (AUTHOR), Koschmieder, Steffen1 (AUTHOR) skoschmieder@ukaachen.de
Publikováno v:
Journal of Hematology & Oncology. 4/2/2019, Vol. 12 Issue 1, p1-16. 16p. 6 Graphs.
Autor:
Schubert, Claudia, Allhoff, Manuel, Tillmann, Stefan, Maié, Tiago, Costa, Ivan, Lipka, Daniel, Mirle Schemionek, Feldberg, Kristina, Baumeister, Julian, Brümmendorf, Tim, Chatain, Nicolas, Koschmieder, Steffen
Figure S6. Confirmation of successful STAT1 or STAT2 knockout. Western blotting of several 32D-BCR-ABL or 32D-JAK2V617F STAT1 or STAT2 knockout clones. STAT2 antibody was used to confirm the knockout, and GAPDH served as the loading control. 32D cell
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6458cde7bc383d4cde0d455df4f0805f
Autor:
Schubert, Claudia, Allhoff, Manuel, Tillmann, Stefan, MaiĂŠ, Tiago, Costa, Ivan, Lipka, Daniel, Mirle Schemionek, Feldberg, Kristina, Baumeister, Julian, BrĂźmmendorf, Tim, Chatain, Nicolas, Koschmieder, Steffen
Supplementary Material & Methods. Table S1. Primer for cloning STAT1 and STAT2 with included restriction sites. Table S2. STAT1 and STAT2 primers for mutagenesis. Table S3. RT-qPCR primer for gene expression analysis. Table S4. Western blot antibodie
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0b5d229914c26eb522ce0c6fb1c18e92
Autor:
Schubert, Claudia, Allhoff, Manuel, Tillmann, Stefan, Maié, Tiago, Costa, Ivan, Lipka, Daniel, Mirle Schemionek, Feldberg, Kristina, Baumeister, Julian, Brümmendorf, Tim, Chatain, Nicolas, Koschmieder, Steffen
Figure S9. Full RT-qPCR panels of tested ISGs. Illustration of the RT-qPCR results of 32D-BCR-ABL- and 32D-JAK2V617F-WT or -STATko or -STAT1(Y/F) and STAT2(Y/F) reconstituted cell clones treated with IFNa (100 U/ml) or left untreated (triplicate), co
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8465a91fb449957f4dbc50ef43b55223
Autor:
Schubert, Claudia, Allhoff, Manuel, Tillmann, Stefan, Maié, Tiago, Costa, Ivan, Lipka, Daniel, Mirle Schemionek, Feldberg, Kristina, Baumeister, Julian, Brümmendorf, Tim, Chatain, Nicolas, Koschmieder, Steffen
Figure S7. Comparison of CRISPR/Cas9 manipulated 32D cell lines treated with 100 U IFNa in survival and titration of lower IFNa dosages. Indicated (A) 32D-BCR-ABL and (B) 32D-JAK2V617F cell lines were analyzed in an MTT assay and treated with 100 U I
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2696d3342c6d6ef738199dd41ff5213b
Autor:
Schubert, Claudia, Allhoff, Manuel, Tillmann, Stefan, Maié, Tiago, Costa, Ivan, Lipka, Daniel, Mirle Schemionek, Feldberg, Kristina, Baumeister, Julian, Brümmendorf, Tim, Chatain, Nicolas, Koschmieder, Steffen
Figure S2. MTT assay of 32D-BCR-ABL and 32D-JAK2V617F cells treated with IFNa. 32D-BCR-ABL-(blue) and 32D-JAK2V617F-(red) positive cells were treated with IFNa (0–104 U/ml) alone (continuous lines) or in combination with 0.1 μM imatinib (IM) or ru
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::af5f59c448b9ba5b97741dbf89ba19b5
Autor:
Schubert, Claudia, Allhoff, Manuel, Tillmann, Stefan, MaiĂŠ, Tiago, Costa, Ivan, Lipka, Daniel, Mirle Schemionek, Feldberg, Kristina, Baumeister, Julian, BrĂźmmendorf, Tim, Chatain, Nicolas, Koschmieder, Steffen
Figure S1. Knockout of STAT1 or STAT2 with CRISPR/Cas9n technology. Four guide RNAs have been generated for STAT1 or STAT2 knockout in 32D-BCR-ABL and 32D-JAK2V617F cells, respectively. Excised exons are given. (PDF 19 kb)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::188d3f1a876855ebacddf9a5ea2c0cd0
Autor:
Schubert, Claudia, Allhoff, Manuel, Tillmann, Stefan, Maié, Tiago, Costa, Ivan, Lipka, Daniel, Mirle Schemionek, Feldberg, Kristina, Baumeister, Julian, Brümmendorf, Tim, Chatain, Nicolas, Koschmieder, Steffen
Figure S4. Effect of extrinsic soluble factors on gene expression in 32D-EV- or 32D-JAK2V617F-positive cells. Supernatant of WEHI-starved 32D-EV- or 32D-JAK2V617F-positive cells was generated overnight, and after removal of the cells, fresh EV (green
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2fc217e2652574cb4c812b54f2779b32
Autor:
Schubert, Claudia, Allhoff, Manuel, Tillmann, Stefan, MaiĂŠ, Tiago, Costa, Ivan, Lipka, Daniel, Mirle Schemionek, Feldberg, Kristina, Baumeister, Julian, BrĂźmmendorf, Tim, Chatain, Nicolas, Koschmieder, Steffen
Figure S3. BCR-ABL reduces ISG expression in 32D cells. Gene expression microarray analysis of 32D-EV, 32D-BCR-ABL, or 32D-JAK2V617F cells. Fold change of gene expression is shown, depicting downregulation of the analyzed gene in blue and upregulatio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b99520ed323e4b1419b99e4c884fcaba